Skip to content

Research at St Andrews

Displacement of the canonical single-stranded DNA-binding protein in the Thermoproteales

Research output: Contribution to journalArticle

Author(s)

Sonia Paytubi ; Stephen McMahon ; Shirley Graham ; Huanting Liu ; Catherine Helen Botting ; Kira S. Makarova ; Eugene V. Kroonin ; Jim Naismith ; Malcolm F White

School/Research organisations

Abstract

Single-stranded DNA binding proteins (SSBs) based on the OB-fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, recombination and repair. We demonstrate that the thermoproteales, a clade of hyperthermophilic crenarchaea, lack a canonical SSB. Instead, they encode a distinct ssDNA-binding protein that we term "ThermoDBP", exemplified by protein Ttx1576 from Thermoproteus tenax. ThermoDBP binds specifically to ssDNA with low sequence specificity. The crystal structure of Ttx1576 reveals a unique fold and mechanism for ssDNA binding, consisting of an extended cleft lined with hydrophobic phenylalanine residues and flanked by basic amino acids. Two ssDNA-binding domains are linked by a coiled-coil leucine zipper. ThermoDBP appears to have displaced the canonical SSB during the diversification of the thermoproteales – a highly unusual example where a “ubiquitous” protein has been lost in evolution.
Close

Details

Original languageEnglish
Pages (from-to)E398-E405
Number of pages8
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number7
Early online date21 Nov 2011
DOIs
StatePublished - 14 Feb 2012

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The Scottish Structural Proteomics Facility: targets, methods and outputs

    Botting, C. H. , Coote, P. J. , Schwarz-Linek, U. , Taylor, G. L. , White, M. F. , Naismith, J. H. & 25 others Oke, M., Carter, L. G., Johnson, K. A., Liu, H., McMahon, S. A., Yan, X., Kerou, M., Weikart, N. D., Kadi, N., Sheikh, M. A., Schmelz, S., Dorward, M., Zawadzki, M., Cozens, C., Falconer, H., Powers, H., Overton, I. M., van Niekerk, C. A., Peng, X., Patel, P., Garrett, R. A., Prangishvili, D., Dryden, D. T., Barton, G. J. & Challis, G. L. 1 Jun 2010 In : Journal of Structural and Functional Genomics. 11, 2, p. 167-180

    Research output: Contribution to journalArticle

  2. AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis

    Schmelz, S., Kadi, N., McMahon, S., Song, L., Oves-Costales, D., Oke, M., Liu, H., Johnson, K. A., Carter, L., Botting, C. H., White, M. F., Challis, G. L. & Naismith, J. Mar 2009 In : Nature Chemical Biology. 5, 3, p. 174-182 9 p.

    Research output: Contribution to journalArticle

  3. Unusual chromophore and cross-links in Ranasmurfin: a blue protein from the foam nests of a tropical frog

    Oke, M., Ching, R. T., Carter, L., Johnson, K. A., Liu, H., McMahon, S., White, M. F., Bloch, C. J., Botting, C. H., Walsh, M. A., Latiff, A. A., Kennedy, M. W., Cooper, A. & Naismith, J. H. 2008 In : Angewandte Chemie International Edition. 47, p. 7853-7856 4 p.

    Research output: Contribution to journalArticle

  4. Taking a molecular motor for a spin: helicase mechanism studied by spin labelling and PELDOR

    Constantinescu Aruxandei, D., Petrovic-Stojanovska, B., Schiemann, O., Naismith, J. & White, M. F. 10 Dec 2015 In : Nucleic Acids Research. Advance Access

    Research output: Contribution to journalArticle

  5. Structural analysis of leader peptide binding enables leader-free cyanobactin processing

    Koehnke, J. A. J. G., Mann, G., Bent, A. F., Ludewig, H., Shirran, S. L., Botting, C. H., Lebl, T., Houssen, W., Jaspers, M. & Naismith, J. Aug 2015 In : Nature Chemical Biology. 11, 8, p. 558-563

    Research output: Contribution to journalArticle

Related by journal

Related by journal

  1. Core disgust is attenuated by ingroup relations

    Reicher, S. D., Templeton, A., Neville, F. G., Ferrari, L. & Drury, J. 26 Jan 2016 In : Proceedings of the National Academy of Sciences of the United States of America.

    Research output: Contribution to journalArticle

  2. Climate challenges, vulnerabilities, and food security

    Nelson, M. C., Ingram, S. E., Dugmore, A. J., Streeter, R. T., Peeples, M. A., McGovern, T. H., Hegmon, M., Arneborg, J., Kintigh, K. W., Brewington, S., Spielmann, K. A., Simpson, I. A., Strawhacker, C., Comeau, L. E. L., Torvinen, A., Madsen, C. K., Hambrecht, G. & Smiarowski, K. 28 Dec 2015 In : Proceedings of the National Academy of Sciences of the United States of America.

    Research output: Contribution to journalArticle

  3. Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting

    Fiddyment, S., Holsinger, B., Ruzzier, C., Devine, A., Binois, A., Albarella, U., Fischer, R., Nichols, E., Curtis, A., Cheese, E., Teasdale, M. D., Checkley-Scott, C., Milner, S. J., Rudy, K. M., Johnson, E. J., Vnouček, J., Garrison, M., McGrory, S., Bradley, D. G. & Collins, M. J. 8 Dec 2015 In : Proceedings of the National Academy of Sciences of the United States of America. 112, 49, p. 15066-15071 6 p.

    Research output: Contribution to journalArticle

  4. Key bioactive reaction products of the NO/H2S interaction are S/N-hybrid species, polysulfides, and nitroxyl

    Cortese-Krott, M. M., Kuhnle, G. G. C., Dyson, A., Fernandez, B. O., Grman, M., DuMond, J. F., Barrow, M. P., McLeod, G., Nakagawa, H., Ondrias, K., Nagy, P., King, S. B., Saavedra, J. E., Keefer, L. K., Singer, M., Kelm, M., Butler, A. R. & Feelisch, M. 25 Aug 2015 In : Proceedings of the National Academy of Sciences of the United States of America. 112, 34, p. 4651-4660 10 p.

    Research output: Contribution to journalArticle

  5. The major evolutionary transitions in individuality

    West, S., Fisher, R., Gardner, A. & Kiers, E. Aug 2015 In : Proceedings of the National Academy of Sciences of the United States of America. 112, 33, p. 10112-10119

    Research output: Contribution to journalArticle

ID: 16016046