Skip to content

Research at St Andrews

Displacement of the canonical single-stranded DNA-binding protein in the Thermoproteales

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

Sonia Paytubi, Stephen McMahon, Shirley Graham, Huanting Liu, Catherine Helen Botting, Kira S. Makarova, Eugene V. Kroonin, Jim Naismith, Malcolm F White

School/Research organisations

Abstract

Single-stranded DNA binding proteins (SSBs) based on the OB-fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, recombination and repair. We demonstrate that the thermoproteales, a clade of hyperthermophilic crenarchaea, lack a canonical SSB. Instead, they encode a distinct ssDNA-binding protein that we term "ThermoDBP", exemplified by protein Ttx1576 from Thermoproteus tenax. ThermoDBP binds specifically to ssDNA with low sequence specificity. The crystal structure of Ttx1576 reveals a unique fold and mechanism for ssDNA binding, consisting of an extended cleft lined with hydrophobic phenylalanine residues and flanked by basic amino acids. Two ssDNA-binding domains are linked by a coiled-coil leucine zipper. ThermoDBP appears to have displaced the canonical SSB during the diversification of the thermoproteales – a highly unusual example where a “ubiquitous” protein has been lost in evolution.
Close

Details

Original languageEnglish
Pages (from-to)E398-E405
Number of pages8
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number7
Early online date21 Nov 2011
DOIs
StatePublished - 14 Feb 2012

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The Scottish Structural Proteomics Facility: targets, methods and outputs

    Oke, M. , Carter, L. G. , Johnson, K. A. , Liu, H. , McMahon, S. A. , Yan, X. , Kerou, M. , Weikart, N. D. , Kadi, N. , Sheikh, M. A. , Schmelz, S. , Dorward, M. , Zawadzki, M. , Cozens, C. , Falconer, H. , Powers, H. , Overton, I. M. , van Niekerk, C. A. , Peng, X. , Patel, P. & 11 others Garrett, R. A., Prangishvili, D., Botting, C. H., Coote, P. J., Dryden, D. T., Barton, G. J., Schwarz-Linek, U., Challis, G. L., Taylor, G. L., White, M. F. & Naismith, J. H. 1 Jun 2010 In : Journal of Structural and Functional Genomics. 11, 2, p. 167-180

    Research output: Contribution to journalArticle

  2. Pironetin reacts covalently with cysteine-316 of α-tubulin to destabilize microtubule

    Yang, J., Wang, Y., Wang, T., Jiang, J., Botting, C. H., Liu, H., Chen, Q., Yang, J., Naismith, J. H., Zhu, X. & Chen, L. 30 Jun 2016 In : Nature Communications. 7, 9 p., 12103

    Research output: Contribution to journalArticle

  3. Mechanism of DNA loading by the DNA repair helicase XPD

    Constantinescu-Aruxandei, D., Petrovic-Stojanovska, B., Penedo, C., White, M. F. & Naismith, J. H. 7 Apr 2016 In : Nucleic Acids Research. 44, 6, p. 2806-2815 10 p.

    Research output: Contribution to journalArticle

  4. Taking a molecular motor for a spin: helicase mechanism studied by spin labelling and PELDOR

    Constantinescu Aruxandei, D., Petrovic-Stojanovska, B., Schiemann, O., Naismith, J. & White, M. F. 10 Dec 2015 In : Nucleic Acids Research. Advance Access

    Research output: Contribution to journalArticle

  5. Structural analysis of leader peptide binding enables leader-free cyanobactin processing

    Koehnke, J. A. J. G., Mann, G., Bent, A. F., Ludewig, H., Shirran, S. L., Botting, C. H., Lebl, T., Houssen, W., Jaspers, M. & Naismith, J. Aug 2015 In : Nature Chemical Biology. 11, 8, p. 558-563

    Research output: Contribution to journalArticle

Related by journal

  1. Synchronous volcanic eruptions and abrupt climate change ~17.7k years ago plausibly linked by stratospheric ozone depletion

    McConnell, J. R. , Burke, A. , Dunbar, N. W. , Köhler, P. , Thomas, J. L. , Arienzo, M. M. , Chellman, N. J. , Maselli, O. J. , Sigl, M. , Adkins, J. F. , Baggenstos, D. , Burkhart, J. F. , Brook, E. J. , Buizert, C. , Cole-Dai, J. , Fudge, T. J. , Knorr, G. , Graf, H-F. , Grieman, M. M. , Iverson, N. & 9 others McGwire, K. C., Mulvaney, R., Paris, G., Rhodes, R. H., Saltzman, E. S., Severinghaus, J. P., Steffensen, J. P., Taylor, K. C. & Winckler, G. 5 Sep 2017 In : Proceedings of the National Academy of Sciences of the United States of America. Early Edition, 6 p.

    Research output: Contribution to journalArticle

  2. Coevolution of cultural intelligence, extended life history, sociality, and brain size in primates

    Street, S. E., Navarrete, A. F., Reader, S. M. & Laland, K. N. 24 Jul 2017 In : Proceedings of the National Academy of Sciences of the United States of America. 114, 30, p. 7908-7914

    Research output: Contribution to journalArticle

  3. The extension of biology through culture

    Whiten, A., Ayala, F., Feldman, M. W. & Laland, K. N. 24 Jul 2017 In : Proceedings of the National Academy of Sciences of the United States of America. Early Edition, 7 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Proceedings of the National Academy of Sciences of the United States of America (Journal)

    Nevels, M. M. (Guest editor)
    Jul 2016 → …

    Activity: Publication peer-review and editorial workEditor of research journal

  2. Proceedings of the National Academy of Sciences of the United States of America (Journal)

    Smith, T. K. (Member of editorial board)
    2012 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

  3. Proceedings of the National Academy of Sciences of the United States of America (Journal)

    Smith, T. K. (Member of editorial board)
    2008 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

  4. Proceedings of the National Academy of Sciences of the United States of America (Journal)

    Miles, G. B. (Reviewer)
    2006 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

ID: 16016046