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Research at St Andrews

Christos Pliotas


Personal details

Profile photoChristos Pliotas
Royal Society of Edinburgh Personal Research Fellow
The Annex, BMS
Room B311
School of Chemistry
Phone: +44 (0)1334 463410

Academic/Professional Qualification

Royal Society of Edinburgh Personal Research Fellow (2016-2021), Biomedical Sciences Research Complex, School of Biology, University of St Andrews (group leader)

Postdoctoral Research Fellow (2011-2015), School of Chemistry, University of St Andrews (J. H. Naismith FRS and O. Schiemann labs) 

PhD in Biochemistry (2007-2010), Institute of Medical Sciences, University of Aberdeen (I. R. Booth lab)

Msc in Medical Physics (2006-2007), Biomedical Physics Department, University of Aberdeen

BSc (Hons) in Physics, University of Athens

Honours and Awards

Peer Review

Research output

Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels

Ackermann, K., Pliotas, C., Valera, S., Naismith, J. & Bode, B. E. 7 Nov 2017 In : Biophysical Journal. 113, 9, p. 1968–1978

Adenosine monophosphate binding stabilizes the KTN domain of the Shewanella denitrificans Kef potassium efflux system

Pliotas, C., Grayer, S. C., Ekkerman, S., Chan, A. K. N., Healy, J., Marius, P., Bartlett, W., Khan, A., Cortopassi, W. A., Chandler, S. A., Rasmussen, T., Benesch, J. L. P., Paton, R. S., Claridge, T. D. W., Miller, S., Booth, I. R., Naismith, J. H. & Conway, S. J. 15 Aug 2017 In : Biochemistry. 56, 32, p. 4219-4234

Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR

Pliotas, C. 10 Aug 2017 A Structure-Function Toolbox for Membrane Transporter and Channels. Ziegler, C. (ed.). Elsevier, Vol. 594, p. 203-242 (Methods in Enzymology)

Enhanced imaging of lipid rich nanoparticles embedded in methylcellulose films for transmission electron microscopy using mixtures of heavy metals

Asadi, J., Ferguson, S., Raja, H., Hacker, C., Marius, P., Ward, R., Pliotas, C., Naismith, J. H. & Lucocq, J. Aug 2017 In : Micron. 99, p. 40-48 9 p.

Spectator no more, the role of the membrane in regulating ion channel function

Pliotas, C. & Naismith, J. H. Aug 2017 In : Current Opinion in Structural Biology. 45, p. 59-66 8 p.

Gating MscS: Structural basis of mechanosensation and the role of lipids in ion channel regulation

Pliotas, C., Dahl, A. C., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Rasmussen, A., Robinson, C. V., Bayley, H., Sansom, M. S., Booth, I. R. & Naismith, J. H. Sep 2016 In : Acta Crystallographica Section A: Foundations and Advances. A72, s35

Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy

Hacker, C., Asadi, J., Pliotas, C., Ferguson, S. G. A., Sherry, L., Marius, P., Tello, J., Jackson, D., Naismith, J. H. & Lucocq, J. M. 4 May 2016 In : Scientific Reports. 6, 13 p., 25275

Accurate extraction of nanometer distances in multimers by pulse EPR

Valera, S., Ackermann, K., Pliotas, C., Huang, H., Naismith, J. H. & Bode, B. E. 24 Mar 2016 In : Chemistry - A European Journal. 22, 14, p. 4700–4703

The role of lipids in mechanosensation

Pliotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R. & Naismith, J. 2015 In : Nature Structural and Molecular Biology. Advance online

Understanding the structural requirements for activators of the Kef bacterial potassium efflux system

Healy, J., Ekkerman, S., Pliotas, C., Richard, M., Bartlett, W., Grayer, S. C., Morris, G. M., Miller, S., Booth, I. R., Conway, S. J. & Rasmussen, T. 6 Mar 2014 In : Biochemistry. 53, 12, p. 1982-1992 11 p.

Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance

Ward, R., Pliotas, C., Branigan, E., Hacker, C., Rasmussen, A., Hagelueken, G., Booth, I. R., Miller, S., Lucocq, J., Naismith, J. & Schiemann, O. 2014 In : Biophysical Journal. 106, 4, p. 834-42 9 p.

Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding

Branigan, E., Pliotas, C., Hageluken, G. & Naismith, J. Nov 2013 In : Nature Protocols. 8, 11, p. 2090-2097 8 p.

Measuring Transmembrane Helix Separation on the MscS Mechanosensitive Channel using Pulsed Electron-Electron Double Resonance (PELDOR) Spectroscopy

Pliotas, C., Ward, R., Branigan, E., Rasmussen, A., Booth, I. R., Schiemann, O. & Naismith, J. H. 29 Jan 2013 In : Biophysical Journal. 104, 2 Supplement 1, p. 468a-468a 1 p.

Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy

Pliotas, C., Ward, R. J., Branigan, E., Rasmussen, A., Hageluken, G., Huang, H., Black, S. S., Booth, I. R., Schiemann, O. & Naismith, J. 2012 In : Proceedings of the National Academy of Sciences of the United States of America. 109, 40, p. E2675-E2682 8 p.

Activation and complex regulation of the Kef potassium efflux system during protection of bacteria against toxic electrophiles

Rasmussen, T., Pliotas, C., Lyngberg, L., Healy, J., Bartlett, W., Miller, S., Roosild, T. P., Castronovo, S., Conway, S. J. & Booth, I. R. 2 Feb 2011 In : Biophysical Journal. 100, 3, p. 102a

Mechanism of ligand-gated potassium efflux in bacterial pathogens

Roosild, T. P., Castronovo, S., Healy, J., Miller, S., Pliotas, C., Rasmussen, T., Bartlett, W., Conway, S. J. & Booth, I. R. 16 Nov 2010 In : Proceedings of the National Academy of Sciences of the United States of America. 107, 46, p. 19784-19789 6 p.

Invited Talks

30th European Crystallography Meeting, ECM

Pliotas, C. (Invited speaker)
1 Sep 2016

Activity: Talk or presentationInvited talk


Frontiers in Integral Membrane Protein Structural Biology

Pliotas, C. (Participant)
5 Oct 20157 Oct 2015

The 47th Annual International Meeting of the ESR Spectroscopy Group of the RSC

Pliotas, C. (Participant)
6 Apr 201410 Apr 2014

Biophysical Society, 57th Annual Meeting

Pliotas, C. (Participant)
2 Feb 20136 Feb 2013

12th Eurasia Conference on Chemical Sciences (EuAsC2S-12)

Pliotas, C. (Speaker)
16 Apr 201221 Apr 2012