Skip to content

Research at St Andrews

16S rRNA gene metabarcoding and TEM reveals different ecological strategies within the genus Neogloboquadrina (planktonic foraminifer)

Research output: Contribution to journalArticle

Author(s)

Clare Bird, Kate F. Darling, Ann D. Russell, Jennifer S. Fehrenbacher, Catherine V. Davis, Andrew Free, Bryne T. Ngwenya

School/Research organisations

Abstract

Uncovering the complexities of trophic and metabolic interactions among microorganisms is essential for the understanding of marine biogeochemical cycling and modelling climate-driven ecosystem shifts. High-throughput DNA sequencing methods provide valuable tools for examining these complex interactions, although this remains challenging, as many microorganisms are difficult to isolate, identify and culture. We use two species of planktonic foraminifera from the climatically susceptible, palaeoceanographically important genus Neogloboquadrina, as ideal test microorganisms for the application of 16S rRNA gene metabarcoding. Neogloboquadrina dutertrei and Neogloboquadrina incompta were collected from the California Current and subjected to either 16S rRNA gene metabarcoding, fluorescence microscopy, or transmission electron microscopy (TEM) to investigate their species-specific trophic interactions and potential symbiotic associations. 53–99% of 16S rRNA gene sequences recovered from two specimens of N. dutertrei were assigned to a single operational taxonomic unit (OTU) from a chloroplast of the phylum Stramenopile. TEM observations confirmed the presence of numerous intact coccoid algae within the host cell, consistent with algal symbionts. Based on sequence data and observed ultrastructure, we taxonomically assign the putative algal symbionts to Pelagophyceae and not Chrysophyceae, as previously reported in this species. In addition, our data shows that N. dutertrei feeds on protists within particulate organic matter (POM), but not on bacteria as a major food source. In total contrast, of OTUs recovered from three N. incompta specimens, 83–95% were assigned to bacterial classes Alteromonadales and Vibrionales of the order Gammaproteobacteria. TEM demonstrates that these bacteria are a food source, not putative symbionts. Contrary to the current view that non-spinose foraminifera are predominantly herbivorous, neither N. dutertrei nor N. incompta contained significant numbers of phytoplankton OTUs. We present an alternative view of their trophic interactions and discuss these results within the context of modelling global planktonic foraminiferal abundances in response to high-latitude climate change.
Close

Details

Original languageEnglish
Article numbere0191653
Number of pages26
JournalPLoS One
Volume13
Issue number1
DOIs
Publication statusPublished - 29 Jan 2018

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The genetic diversity, morphology, biogeography, and taxonomic designations of Ammonia (Foraminifera) in the Northeast Atlantic

    Bird, C., Schweizer, M., Roberts, A., Austin, W. E. N., Knudsen, K. L., Evans, K. M., Filipsson, H. L., Sayer, M. D. J., Geslin, E. & Darling, K. F., 19 Feb 2019, In : Marine Micropaleontology.

    Research output: Contribution to journalArticle

  2. Assessing proxy signatures of temperature, salinity, and hypoxia in the Baltic Sea through foraminifera-based geochemistry and faunal assemblages

    Groeneveld, J., Filipsson, H. L., Austin, W. E. N., Darling, K., McCarthy, D., Krupinski, N. B. Q., Bird, C. & Schweizer, M., 7 Sep 2018, In : Journal of Micropalaeontology. 37, 2, p. 403-429 27 p.

    Research output: Contribution to journalArticle

  3. Distribution and ecology of planktic foraminifera in the North Pacific: implications for paleo-reconstructions

    Taylor, B. J., Rae, J. W. B., Gray, W. R., Darling, K., Burke, A., Gersonde, R., Abelmann, A., Maier, E., Esper, O. & Ziveri, P., 1 Jul 2018, In : Quaternary Science Reviews. 191, p. 256-274 19 p.

    Research output: Contribution to journalArticle

  4. Genetic diversity and ecology of the planktonic foraminifers Globigerina bulloides, Turborotalita quinqueloba and Neogloboquadrina pachyderma off the Oman margin during the late SW Monsoon

    Darling, K. F., Wade, C. M., Siccha, M., Trommer, G., Schulz, H., Abdolalipour, S. & Kurasawa, A., Dec 2017, In : Marine Micropaleontology. 137, p. 64-77

    Research output: Contribution to journalArticle

  5. Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology

    Morard, R., Lejzerowicz, F., Darling, K. F., Lecroq-Bennet, B., Winther Pedersen, M., Orlando, L., Pawlowski, J., Mulitza, S., de Vargas, C. & Kucera, M., 6 Jun 2017, In : Biogeosciences. 14, 11, p. 2741-2754 14 p.

    Research output: Contribution to journalArticle

Related by journal

  1. PLoS ONE (Journal)

    Joanna Louise Kershaw (Member of editorial board)
    18 Mar 2019

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. PLoS ONE (Journal)

    Catharine Penelope Cross (Member of editorial board)
    1 Sep 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  3. PLoS ONE (Journal)

    Will Cresswell (Reviewer)
    23 May 2018

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  4. PLoS ONE (Journal)

    Barbara Dritschel (Member of editorial board)
    May 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  5. PLoS ONE (Journal)

    Simon Young (Reviewer)
    7 Jun 201719 Jun 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Agreement between primary care and hospital diagnosis of schizophrenia and bipolar disorder: a cross-sectional, observational study using record linkage

    O’Neill, B., Kalia, S., Aliarzadeh, B., Moineddin, R., Fung, W. L. A., Sullivan, F., Maloul, A., Bernard, S. & Greiver, M., 7 Jan 2019, In : PLoS ONE. 14, 1, 15 p., e0210214.

    Research output: Contribution to journalArticle

  2. Animal behaviour in a human world: a crowdsourcing study on horses that open door and gate mechanisms

    Krueger, K., Esch, L. & Byrne, R., 26 Jun 2019, In : PLoS ONE. 14, 6, 20 p., e0218954.

    Research output: Contribution to journalArticle

  3. Correlates of social role and conflict severity in wild vervet monkey agonistic screams

    Mercier, S., Déaux, E. C., van de Waal, E., Bono, A. E. J. & Zuberbuhler, K., 1 May 2019, In : PLoS One. 14, 5, 20 p., e0214640.

    Research output: Contribution to journalArticle

  4. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

    Mohamed, N., Timofeyeva, Y., Jamrozy, D., Rojas, E., Hao, L., Silmon de Monerri, N. C., Hawkins, J., Singh, G., Cai, B., Liberator, P., Sebastian, S., Donald, R. G. K., Scully, I. L., Jones, C. H., Creech, C. B., Thomsen, I., Parkhill, J., Peacock, S. J., Jansen, K. U., Holden, M. T. G. & 1 othersAnderson, A. S., 14 Jan 2019, In : PLoS ONE. 14, 1, 33 p., e0208356.

    Research output: Contribution to journalArticle

  5. Orangutans (Pongo abelii) make flexible decisions relative to reward quality and tool functionality in a multi-dimensional tool-use task

    Laumer, I. B., Auersperg, A. M. I., Bugnyar, T. & Call, J., 13 Feb 2019, In : PLoS ONE. 14, 2, 13 p.

    Research output: Contribution to journalArticle

ID: 252191987