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17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

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17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. / Zafirovic, Sonja; Obradovic, Milan; Sudar-Milovanovic, Emina; Jovanovic, Aleksandra; Stanimirovic, Julijana; Stewart, Alan J.; Pitt, Samantha J.; Isenovic, Esma R.

In: Molecular and Cellular Endocrinology, Vol. 446, 05.05.2017, p. 12-20.

Research output: Contribution to journalArticle

Harvard

Zafirovic, S, Obradovic, M, Sudar-Milovanovic, E, Jovanovic, A, Stanimirovic, J, Stewart, AJ, Pitt, SJ & Isenovic, ER 2017, '17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats' Molecular and Cellular Endocrinology, vol. 446, pp. 12-20. https://doi.org/10.1016/j.mce.2017.02.001

APA

Zafirovic, S., Obradovic, M., Sudar-Milovanovic, E., Jovanovic, A., Stanimirovic, J., Stewart, A. J., ... Isenovic, E. R. (2017). 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. Molecular and Cellular Endocrinology, 446, 12-20. https://doi.org/10.1016/j.mce.2017.02.001

Vancouver

Zafirovic S, Obradovic M, Sudar-Milovanovic E, Jovanovic A, Stanimirovic J, Stewart AJ et al. 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. Molecular and Cellular Endocrinology. 2017 May 5;446:12-20. https://doi.org/10.1016/j.mce.2017.02.001

Author

Zafirovic, Sonja ; Obradovic, Milan ; Sudar-Milovanovic, Emina ; Jovanovic, Aleksandra ; Stanimirovic, Julijana ; Stewart, Alan J. ; Pitt, Samantha J. ; Isenovic, Esma R. / 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. In: Molecular and Cellular Endocrinology. 2017 ; Vol. 446. pp. 12-20.

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@article{39cf3c6ce256400baf562299711c774f,
title = "17{\ss}-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats",
abstract = "The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42{\%} fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.",
keywords = "Estradiol, Obesity, Myocardial metabolism, Cardiovascular diseases, Inducible nitric oxide synthase",
author = "Sonja Zafirovic and Milan Obradovic and Emina Sudar-Milovanovic and Aleksandra Jovanovic and Julijana Stanimirovic and Stewart, {Alan J.} and Pitt, {Samantha J.} and Isenovic, {Esma R.}",
note = "This work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia.",
year = "2017",
month = "5",
day = "5",
doi = "10.1016/j.mce.2017.02.001",
language = "English",
volume = "446",
pages = "12--20",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

AU - Zafirovic, Sonja

AU - Obradovic, Milan

AU - Sudar-Milovanovic, Emina

AU - Jovanovic, Aleksandra

AU - Stanimirovic, Julijana

AU - Stewart, Alan J.

AU - Pitt, Samantha J.

AU - Isenovic, Esma R.

N1 - This work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia.

PY - 2017/5/5

Y1 - 2017/5/5

N2 - The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.

AB - The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.

KW - Estradiol

KW - Obesity

KW - Myocardial metabolism

KW - Cardiovascular diseases

KW - Inducible nitric oxide synthase

UR - http://www.sciencedirect.com/science/article/pii/S0303720717300771

U2 - 10.1016/j.mce.2017.02.001

DO - 10.1016/j.mce.2017.02.001

M3 - Article

VL - 446

SP - 12

EP - 20

JO - Molecular and Cellular Endocrinology

T2 - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

ER -

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ID: 249051761