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A Dimeric Rep Protein Initiates Replication of a Linear Archaeal Virus Genome: Implications for the Rep Mechanism and Viral Replication

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Author(s)

Muse Oke, Melina Kerou, Huanting Liu, Xu Peng, Roger A. Garrett, David Prangishvili, James H. Naismith, Malcolm F. White

School/Research organisations

Abstract

The Rudiviridae are a family of rod-shaped archaeal viruses with covalently closed, linear double-stranded DNA (dsDNA) genomes. Their replication mechanisms remain obscure, although parallels have been drawn to the Poxviridae and other large cytoplasmic eukaryotic viruses. Here we report that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between an active-site tyrosine and the 5' end of the DNA, releasing a 3' DNA end as a primer for DNA synthesis. The enzyme can also catalyze the joining reaction that is necessary to reseal the DNA hairpin and terminate replication. The dimeric structure points to a simple mechanism through which two closely positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral replication are discussed.

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Original languageEnglish
Pages (from-to)925-931
Number of pages7
JournalJournal of Virology
Volume85
Issue number2
DOIs
Publication statusPublished - Jan 2011

    Research areas

  • ARCHAEA, DNA REPLICATION, VIRUS, ENDONUCLEASE DOMAIN, EUKARYAL VIRUSES, ACTIVE-SITE, SIRV1, CLEAVAGE, REP, ORIGIN

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