Skip to content

Research at St Andrews

A gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurements

Research output: Contribution to journalArticle

Open Access Status

  • Embargoed (until 18/02/20)

Author(s)

Anokhi Shah, Amandine Roux, Matthieu Starck, Jackie A. Mosely, Michael Stevens, David G. Norman, Robert I. Hunter, Hassane El Mkami, Graham Smith, David Parker, Janet E. Lovett

School/Research organisations

Abstract

The design, synthesis, and application of a nine-coordinate gadolinium(III)-containing spin label, [Gd.sTPATCN]-SL, for use in nanometer-distance measurement experiments by EPR spectroscopy is presented. The spin label links to cysteines via a short thioether tether and has a narrow central transition indicative of small zero-field splitting (ZFS). A protein homodimer, TRIM25cc, was selectively labeled with [Gd.sTPATCN]-SL (70%) and a nitroxide (30%) under mild conditions and measured using the double electron electron resonance (DEER) technique with both commercial Q-band and home-built W-band spectrometers. The label shows great promise for increasing the sensitivity of DEER measurements through both its favorable relaxation parameters and the large DEER modulation depth at both Q- and W-band for the inter-Gd(III) DEER measurement which, at 9%, is the largest recorded under these conditions.
Close

Details

Original languageEnglish
JournalInorganic Chemistry
VolumeArticle ASAP
Early online date18 Feb 2019
DOIs
Publication statusE-pub ahead of print - 18 Feb 2019

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The use of composite pulses for improving DEER signal at 94 GHz

    Motion, C. L., Cassidy, S. L., Cruickshank, P. A. S., Hunter, R. I., Bolton, D. R., El Mkami, H., Van Doorslaer, S., Lovett, J. E. & Smith, G. M., May 2017, In : Journal of Magnetic Resonance. 278, p. 122-133 12 p.

    Research output: Contribution to journalArticle

  2. DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR

    Motion, C. L., Lovett, J. E., Bell, S., Cassidy, S. L., Cruickshank, P. A. S., Bolton, D. R., Hunter, R. I., El Mkami, H., Van Doorslaer, S. & Smith, G. M., Apr 2016, In : Journal of Physical Chemistry Letters. 7, 8, p. 1411-1415

    Research output: Contribution to journalArticle

  3. Instrumentation for high sensitivity, high power, millimetre wave, electron paramagnetic resonance

    Smith, G. M., Robertson, D. A., Bolton, D. R., Cruickshank, P. A. S., Motion, C., McKay, J., El Mkami, H., Wylde, R. & Hunter, R. I., 31 Mar 2016, IET Colloquium on Millimetre-Wave and Terahertz Engineering & Technology 2016. 1 ed. London: Institution of Engineering and Technology, Vol. 2016. 5 p.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  4. Distance measurement of a noncovalently bound Y@C82 Pair with double electron electron resonance spectroscopy

    Gil-Ramírez, G., Shah, A., El Mkami, H., Porfyrakis, K., Briggs, A. D., Morton, J. J. L., Anderson, H. L. & Lovett, J. E., 20 Jun 2018, In : Journal of the American Chemical Society. 140, 24, p. 7420-7424

    Research output: Contribution to journalArticle

  5. Smoothly profiled quasi-optical output launcher for a W-band gyro-TWA

    Zhang, L., He, W., Donaldson, C. R., Cross, A. W., Smith, G. M., Robertson, D. A. & Hunter, R. I., 16 Oct 2017, 2017 10th UK-Europe-China Workshop on Millimetre Waves and Terahertz Technologies, UCMMT 2017. Institute of Electrical and Electronics Engineers Inc., 8068473

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

Related by journal

  1. Crystallographic insights into the behavior of highly acidic metal cations in ionic liquids from ractions of titanium tetrachloride with [1-butyl-3-methylimidazolium][X] ionic liquids (X = chloride, bromide, tetrafluoroborate)

    Mishra, M. K., Kelley, S. P., Dilip, M., Vaid, T. P., Cordes, D. B., Griffin, S. T. & Rogers, R. D., 4 Feb 2019, In : Inorganic Chemistry. 58, 3, p. 1764-1773

    Research output: Contribution to journalArticle

  2. A study of through-space and through-bond JPP coupling in a rigid nonsymmetrical bis(phosphine) and its metal complexes

    Chalmers, B. A., Nejman, P. L., Llewellyn, A. V., Felaar, A. M., Griffiths, B. L., Portman, E. I., Gordon, E-J. L., Fan, K. J. H., Woollins, J. D., Buehl, M., Malkina, O. L., Cordes, D. B., Slawin, A. M. Z. & Kilian, P., 19 Mar 2018, In : Inorganic Chemistry. 57, 6, p. 3387-3398 12 p.

    Research output: Contribution to journalArticle

  3. Alkaline-earth rhodium hydroxides: synthesis, structures and thermal decomposition to complex oxides

    Cook, D. S., Clarkson, G. J., Dawson, D. M., Ashbrook, S. E., Fisher, J. M., Thompsett, D., Pickup, D. M., Chadwick, A. V. & Walton, R. I., 14 Aug 2018, In : Inorganic Chemistry. In press

    Research output: Contribution to journalArticle

  4. Chitosan immobilization on Bio-MOF nanostructures: a biocompatible pH-responsive nanocarrier for doxorubicin release on MCF-7 cell lines of human breast cancer

    Abazari, R., Mahjoub, A. R., Ataei, F., Morsali, A., Carpenter-Warren, C. L., Mehdizadeh, K. & Slawin, A. M. Z., 5 Nov 2018, In : Inorganic Chemistry. 57, 21, p. 13364-13379 16 p.

    Research output: Contribution to journalArticle

ID: 257572157