Skip to content

Research at St Andrews

A marine viral halogenase that iodinates diverse substrates

Research output: Contribution to journalArticlepeer-review

Author(s)

Danai S. Gkotsi, Hannes Ludewig, Sunil V. Sharma, Jack A. Connolly, Jagwinder Dhaliwal, Yunpeng Wang, William P. Unsworth, Richard J. K. Taylor, Matthew M. W. McLachlan, Stephen Shanahan, James H. Naismith, Rebecca J. M. Goss

School/Research organisations

Abstract

Oceanic cyanobacteria are the most abundant oxygen-generating phototrophs on our planet and are therefore important to life. These organisms are infected by viruses called cyanophages, which have recently shown to encode metabolic genes that modulate host photosynthesis, phosphorus cycling and nucleotide metabolism. Herein we report the characterization of a wild-type flavin-dependent viral halogenase (VirX1) from a cyanophage. Notably, halogenases have been previously associated with secondary metabolism, tailoring natural products. Exploration of this viral halogenase reveals it capable of regioselective halogenation of a diverse range of substrates with a preference for forming aryl iodide species; this has potential implications for the metabolism of the infected host. Until recently, a flavin-dependent halogenase that is capable of iodination in vitro had not been reported. VirX1 is interesting from a biocatalytic perspective as it shows strikingly broad substrate flexibility and a clear preference for iodination, as illustrated by kinetic analysis. These factors together render it an attractive tool for synthesis.
Close

Details

Original languageEnglish
Pages (from-to)1091–1097
Number of pages7
JournalNature Chemistry
Volume11
Issue number12
DOIs
Publication statusPublished - 14 Oct 2019

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. SynBio-SynChem approaches to diversifying the pacidamycins through the exploitation of an observed Pictet-Spengler reaction

    Cartmell, C., Abou Fayad, A., Lynch, R., Sharma, S., Hauck, N., Gust, B. & Goss, R. J. M., 15 Feb 2021, In: ChemBioChem. 22, 4, p. 712-716 6 p.

    Research output: Contribution to journalArticlepeer-review

  2. Synthesis and conformational analysis of fluorinated uridine analogues provide insight into a neighbouring-group participation mechanism

    Michailidou, F., Lebl, T., Slawin, A. M. Z., Sharma, S. V., Brown, M. & Goss, R., 25 Nov 2020, In: Molecules. 25, 23, 13 p., 5513.

    Research output: Contribution to journalArticlepeer-review

  3. Natural products incorporating pyrimidine nucleosides

    Michailidou, F., Burnett, D. A., Sharma, S. V., Van Lanen, S. & Goss, R., 20 Feb 2020, Reference module in chemistry, molecular sciences and chemical engineering. Elsevier Inc.

    Research output: Chapter in Book/Report/Conference proceedingEntry for encyclopedia/dictionary

  4. Buchwald Hartwig diversification of unprotected halotryptophans, halotryptophan containing tripeptides and the natural product barettin in aqueous conditions

    Renault, Y. J. G., Lynch, R., Marelli, E., Sharma, S. V., Pubill Ulldemolins, C., Sharp, J., Cartmell, C., Cárdenas, P. & Goss, R. J. M., 8 Oct 2019, In: Chemical Communications. Advance Article, 4 p.

    Research output: Contribution to journalReview articlepeer-review

  5. Heck diversification of indole‐based substrates under aqueous conditions: from indoles to unprotected halo‐tryptophans and halo‐tryptophans in natural product derivatives

    Pubill-Ulldemolins, C., Sharma, S. V., Cartmell, C., Zhao, J., Cárdenas, P. & Goss, R. J. M., 14 Aug 2019, In: Chemistry - A European Journal. 25, 46, p. 10866-10875 11 p.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. A tale of two tables

    Gil, M. P. & Zysman-Colman, E., Sep 2019, In: Nature Chemistry. 11, 9, p. 757-759 3 p.

    Research output: Contribution to journalComment/debatepeer-review

  2. Safe, sustainable and scalable

    Johnston, C. P., 24 Apr 2019, In: Nature Chemistry. 11, 5, p. 400-401

    Research output: Contribution to journalBook/Film/Article review

  3. Hydrolytic stability in hemilabile metal–organic frameworks

    McHugh, L. N., McPherson, M. J., McCormick, L. J., Morris, S. A., Wheatley, P. S., Teat, S. J., McKay, D., Dawson, D. M., Sansome, C. E. F., Ashbrook, S. E., Stone, C. A., Smith, M. W. & Morris, R. E., Nov 2018, In: Nature Chemistry. 10, 11, p. 1096–1102 7 p.

    Research output: Contribution to journalArticlepeer-review

  4. In situ solid-state NMR and XRD studies of the ADOR process and the unusual structure of zeolite IPC-6

    Morris, S. A., Bignami, G. P. M., Tian, Y., Navarro-Rojas, M., Firth, D. S., Cejka, J., Wheatley, P. S., Dawson, D. M., Slawinski, W. A., Wragg, D. S., Morris, R. E. & Ashbrook, S. E., Oct 2017, In: Nature Chemistry. 9, 10, p. 1012-1018 7 p.

    Research output: Contribution to journalArticlepeer-review

ID: 261904941

Top