Skip to content

Research at St Andrews

A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy

Research output: Contribution to journalArticlepeer-review

Author(s)

Ronan M. Valentine, Sally H. Ibbotson, C. Tom A. Brown, Harry Moseley, Kenneth Wood

School/Research organisations

Abstract

The characteristics of protoporphyrin IX (PPIX) fluorescence in superficial basal cell carcinoma (sBCC) and carcinoma in situ (Bowen's Disease, BD) following application of 5-aminolaevulinic acid (5-ALA) and its methyl ester (methyl aminolevulinate [MAL]) before, during and after photodynamic therapy (PDT) were investigated in 40 patients. Photosensitizer prodrug penetration can limit PDT efficacy and understanding the characteristics of PPIX fluorescence through fluorescence spectroscopy, may improve knowledge of photosensitizer delivery. Fluorescence intensity was assessed quantitatively, and the rate of photobleaching was determined by fitting an exponential decay. As a secondary end-point, PDT-induced pain was also measured continuously during treatment using a novel hand-held device, known as a pain logger. In vivo PPIX fluorescence was shown to decrease during irradiation, allowing the in vivo photobleaching of PPIX to be monitored. No significant difference was found between ALA- or MAL-induced PPIX fluorescence in lesions of sBCC and BD (P > 0.05), indicating no detectable difference in PPIX kinetics for the two prodrugs as assessed by these measures. Pain, as assessed by the logger device, showed high interindividual variability and pain levels tended to be higher initially, decreasing during treatment. No difference was seen in pain experienced during ALA-or MAL-PDT (P > 0.05).

Close

Details

Original languageEnglish
Pages (from-to)242-249
Number of pages8
JournalPhotochemistry and Photobiology
Volume87
Issue number1
DOIs
Publication statusPublished - Jan 2011

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Development of a predictive Monte Carlo radiative transfer model for ablative fractional skin lasers

    McMillan, L., O'Mahoney, P., Feng, K., Zhou, K., Barnard, I. R. M., Li, C., Ibbotson, S., Eadie, E., Brown, C. T. A. & Wood, K., Jul 2021, In: Lasers in Surgery and Medicine. 53, 5, p. 731-740 10 p.

    Research output: Contribution to journalArticlepeer-review

  2. Could psoralen plus ultraviolet A1 (“PUVA1”) work? Depth penetration achieved by phototherapy lamps

    Barnard, I. R. M., Eadie, E., McMillan, L., Moseley, H., Brown, T., Wood, K. & Dawe, R., Mar 2020, In: British Journal of Dermatology. 182, 3, p. 813-814 2 p.

    Research output: Contribution to journalLetterpeer-review

  3. Monte Carlo tissue ablation model

    McMillan, L., Brown, C. T. A., Wood, K. & Barnard, I. R. M., 2019

    Research output: Non-textual formSoftware

  4. A novel light source with tuneable uniformity of light distribution for artificial daylight photodynamic therapy

    O’Mahoney, P., Haigh, N., Wood, K., Brown, C. T. A., Ibbotson, S. & Eadie, E., Sep 2018, In: Photodiagnosis and Photodynamic Therapy. 23, p. 144-150 7 p.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Air disinfection with germicidal ultraviolet: for this pandemic and the next

    Bergman, R., Brenner, D., Buonanno, M., Eadie, E., Forbes, P. D., Jensen, P., Nardell, E. A., Sliney, D., Vincent, R., Welch, D. & Wood, K., 18 May 2021, In: Photochemistry and Photobiology. 97, 3, p. 464-465

    Research output: Contribution to journalEditorialpeer-review

  2. Extreme exposure to filtered far-UVC: a case study

    Eadie, E., Barnard, I. M. R., Ibbotson, S. H. & Wood, K., 3 Feb 2021, In: Photochemistry and Photobiology. Early view

    Research output: Contribution to journalSpecial issuepeer-review

  3. Quantifying direct DNA damage in the basal layer of skin exposed to UV radiation from sunbeds

    Barnard, I. R. M., Tierney, P., Campbell, C. L., McMillan, L., Moseley, H., Eadie, E., Brown, C. T. A. & Wood, K., Sep 2018, In: Photochemistry and Photobiology. 94, 5, p. 1017-1025 9 p.

    Research output: Contribution to journalArticlepeer-review

  4. The application of a compact multispectral imaging system with integrated excitation source to in vivo monitoring of fluorescence during topical photodynamic therapy of superficial skin cancers

    Hewett, J., Nadeau, V., Ferguson, J., Moseley, H., Ibbotson, S., Allen, J. W., Sibbett, W. & Padgett, M., Mar 2001, In: Photochemistry and Photobiology. 73, 3, p. 278-282 5 p.

    Research output: Contribution to journalArticlepeer-review

ID: 24986604

Top