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A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy

Research output: Contribution to journalArticlepeer-review

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A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy. / Valentine, Ronan M.; Ibbotson, Sally H.; Brown, C. Tom A.; Moseley, Harry; Wood, Kenneth.

In: Photochemistry and Photobiology, Vol. 87, No. 1, 01.2011, p. 242-249.

Research output: Contribution to journalArticlepeer-review

Harvard

Valentine, RM, Ibbotson, SH, Brown, CTA, Moseley, H & Wood, K 2011, 'A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy', Photochemistry and Photobiology, vol. 87, no. 1, pp. 242-249. https://doi.org/10.1111/j.1751-1097.2010.00829.x

APA

Valentine, R. M., Ibbotson, S. H., Brown, C. T. A., Moseley, H., & Wood, K. (2011). A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy. Photochemistry and Photobiology, 87(1), 242-249. https://doi.org/10.1111/j.1751-1097.2010.00829.x

Vancouver

Valentine RM, Ibbotson SH, Brown CTA, Moseley H, Wood K. A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy. Photochemistry and Photobiology. 2011 Jan;87(1):242-249. https://doi.org/10.1111/j.1751-1097.2010.00829.x

Author

Valentine, Ronan M. ; Ibbotson, Sally H. ; Brown, C. Tom A. ; Moseley, Harry ; Wood, Kenneth. / A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy. In: Photochemistry and Photobiology. 2011 ; Vol. 87, No. 1. pp. 242-249.

Bibtex - Download

@article{d13506f770f94343afacb4792604358e,
title = "A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy",
abstract = "The characteristics of protoporphyrin IX (PPIX) fluorescence in superficial basal cell carcinoma (sBCC) and carcinoma in situ (Bowen's Disease, BD) following application of 5-aminolaevulinic acid (5-ALA) and its methyl ester (methyl aminolevulinate [MAL]) before, during and after photodynamic therapy (PDT) were investigated in 40 patients. Photosensitizer prodrug penetration can limit PDT efficacy and understanding the characteristics of PPIX fluorescence through fluorescence spectroscopy, may improve knowledge of photosensitizer delivery. Fluorescence intensity was assessed quantitatively, and the rate of photobleaching was determined by fitting an exponential decay. As a secondary end-point, PDT-induced pain was also measured continuously during treatment using a novel hand-held device, known as a pain logger. In vivo PPIX fluorescence was shown to decrease during irradiation, allowing the in vivo photobleaching of PPIX to be monitored. No significant difference was found between ALA- or MAL-induced PPIX fluorescence in lesions of sBCC and BD (P > 0.05), indicating no detectable difference in PPIX kinetics for the two prodrugs as assessed by these measures. Pain, as assessed by the logger device, showed high interindividual variability and pain levels tended to be higher initially, decreasing during treatment. No difference was seen in pain experienced during ALA-or MAL-PDT (P > 0.05).",
author = "Valentine, {Ronan M.} and Ibbotson, {Sally H.} and Brown, {C. Tom A.} and Harry Moseley and Kenneth Wood",
year = "2011",
month = jan,
doi = "10.1111/j.1751-1097.2010.00829.x",
language = "English",
volume = "87",
pages = "242--249",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A Quantitative Comparison of 5-Aminolaevulinic Acid- and Methyl Aminolevulinate-Induced Fluorescence, Photobleaching and Pain During Photodynamic Therapy

AU - Valentine, Ronan M.

AU - Ibbotson, Sally H.

AU - Brown, C. Tom A.

AU - Moseley, Harry

AU - Wood, Kenneth

PY - 2011/1

Y1 - 2011/1

N2 - The characteristics of protoporphyrin IX (PPIX) fluorescence in superficial basal cell carcinoma (sBCC) and carcinoma in situ (Bowen's Disease, BD) following application of 5-aminolaevulinic acid (5-ALA) and its methyl ester (methyl aminolevulinate [MAL]) before, during and after photodynamic therapy (PDT) were investigated in 40 patients. Photosensitizer prodrug penetration can limit PDT efficacy and understanding the characteristics of PPIX fluorescence through fluorescence spectroscopy, may improve knowledge of photosensitizer delivery. Fluorescence intensity was assessed quantitatively, and the rate of photobleaching was determined by fitting an exponential decay. As a secondary end-point, PDT-induced pain was also measured continuously during treatment using a novel hand-held device, known as a pain logger. In vivo PPIX fluorescence was shown to decrease during irradiation, allowing the in vivo photobleaching of PPIX to be monitored. No significant difference was found between ALA- or MAL-induced PPIX fluorescence in lesions of sBCC and BD (P > 0.05), indicating no detectable difference in PPIX kinetics for the two prodrugs as assessed by these measures. Pain, as assessed by the logger device, showed high interindividual variability and pain levels tended to be higher initially, decreasing during treatment. No difference was seen in pain experienced during ALA-or MAL-PDT (P > 0.05).

AB - The characteristics of protoporphyrin IX (PPIX) fluorescence in superficial basal cell carcinoma (sBCC) and carcinoma in situ (Bowen's Disease, BD) following application of 5-aminolaevulinic acid (5-ALA) and its methyl ester (methyl aminolevulinate [MAL]) before, during and after photodynamic therapy (PDT) were investigated in 40 patients. Photosensitizer prodrug penetration can limit PDT efficacy and understanding the characteristics of PPIX fluorescence through fluorescence spectroscopy, may improve knowledge of photosensitizer delivery. Fluorescence intensity was assessed quantitatively, and the rate of photobleaching was determined by fitting an exponential decay. As a secondary end-point, PDT-induced pain was also measured continuously during treatment using a novel hand-held device, known as a pain logger. In vivo PPIX fluorescence was shown to decrease during irradiation, allowing the in vivo photobleaching of PPIX to be monitored. No significant difference was found between ALA- or MAL-induced PPIX fluorescence in lesions of sBCC and BD (P > 0.05), indicating no detectable difference in PPIX kinetics for the two prodrugs as assessed by these measures. Pain, as assessed by the logger device, showed high interindividual variability and pain levels tended to be higher initially, decreasing during treatment. No difference was seen in pain experienced during ALA-or MAL-PDT (P > 0.05).

U2 - 10.1111/j.1751-1097.2010.00829.x

DO - 10.1111/j.1751-1097.2010.00829.x

M3 - Article

VL - 87

SP - 242

EP - 249

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 1

ER -

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