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A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species

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A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species. / Boonsilp, Siriphan; Thaipadungpanit, Janjira; Amornchai, Premjit; Wuthiekanun, Vanaporn; Bailey, Mark S.; Holden, Matthew; Zhang, Cuicai; Jiang, Xiugao; Koizumi, Nobuo; Taylor, Kyle; Galloway, Renee; Hoffmaster, Alex R.; Craig, Scott; Smythe, Lee D.; Hartskeerl, Rudy A.; Day, Nicholas P.; Chantratita, Narisara; Feil, Edward J.; Aanensen, David M.; Spratt, Brian G.; Peacock, Sharon J.

In: PLoS Neglected Tropical Diseases, Vol. 7, No. 1, e1954, 01.2013.

Research output: Contribution to journalArticle

Harvard

Boonsilp, S, Thaipadungpanit, J, Amornchai, P, Wuthiekanun, V, Bailey, MS, Holden, M, Zhang, C, Jiang, X, Koizumi, N, Taylor, K, Galloway, R, Hoffmaster, AR, Craig, S, Smythe, LD, Hartskeerl, RA, Day, NP, Chantratita, N, Feil, EJ, Aanensen, DM, Spratt, BG & Peacock, SJ 2013, 'A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species', PLoS Neglected Tropical Diseases, vol. 7, no. 1, e1954. https://doi.org/10.1371/journal.pntd.0001954

APA

Boonsilp, S., Thaipadungpanit, J., Amornchai, P., Wuthiekanun, V., Bailey, M. S., Holden, M., ... Peacock, S. J. (2013). A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species. PLoS Neglected Tropical Diseases, 7(1), [e1954]. https://doi.org/10.1371/journal.pntd.0001954

Vancouver

Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Bailey MS, Holden M et al. A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species. PLoS Neglected Tropical Diseases. 2013 Jan;7(1). e1954. https://doi.org/10.1371/journal.pntd.0001954

Author

Boonsilp, Siriphan ; Thaipadungpanit, Janjira ; Amornchai, Premjit ; Wuthiekanun, Vanaporn ; Bailey, Mark S. ; Holden, Matthew ; Zhang, Cuicai ; Jiang, Xiugao ; Koizumi, Nobuo ; Taylor, Kyle ; Galloway, Renee ; Hoffmaster, Alex R. ; Craig, Scott ; Smythe, Lee D. ; Hartskeerl, Rudy A. ; Day, Nicholas P. ; Chantratita, Narisara ; Feil, Edward J. ; Aanensen, David M. ; Spratt, Brian G. ; Peacock, Sharon J. / A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species. In: PLoS Neglected Tropical Diseases. 2013 ; Vol. 7, No. 1.

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@article{9084975e11164deaa7a7d18272217334,
title = "A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species",
abstract = "Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.",
keywords = "Genetic diversity, Interrogans, Epidemiology, Relatedness",
author = "Siriphan Boonsilp and Janjira Thaipadungpanit and Premjit Amornchai and Vanaporn Wuthiekanun and Bailey, {Mark S.} and Matthew Holden and Cuicai Zhang and Xiugao Jiang and Nobuo Koizumi and Kyle Taylor and Renee Galloway and Hoffmaster, {Alex R.} and Scott Craig and Smythe, {Lee D.} and Hartskeerl, {Rudy A.} and Day, {Nicholas P.} and Narisara Chantratita and Feil, {Edward J.} and Aanensen, {David M.} and Spratt, {Brian G.} and Peacock, {Sharon J.}",
note = "This study was funded by the Wellcome Trust. BGS and DMA were funded by Wellcome Trust grant 089472. SJP receives support from the NIHR Cambridge Biomedical Research Centre",
year = "2013",
month = "1",
doi = "10.1371/journal.pntd.0001954",
language = "English",
volume = "7",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2735",
publisher = "Public Library of Science",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species

AU - Boonsilp, Siriphan

AU - Thaipadungpanit, Janjira

AU - Amornchai, Premjit

AU - Wuthiekanun, Vanaporn

AU - Bailey, Mark S.

AU - Holden, Matthew

AU - Zhang, Cuicai

AU - Jiang, Xiugao

AU - Koizumi, Nobuo

AU - Taylor, Kyle

AU - Galloway, Renee

AU - Hoffmaster, Alex R.

AU - Craig, Scott

AU - Smythe, Lee D.

AU - Hartskeerl, Rudy A.

AU - Day, Nicholas P.

AU - Chantratita, Narisara

AU - Feil, Edward J.

AU - Aanensen, David M.

AU - Spratt, Brian G.

AU - Peacock, Sharon J.

N1 - This study was funded by the Wellcome Trust. BGS and DMA were funded by Wellcome Trust grant 089472. SJP receives support from the NIHR Cambridge Biomedical Research Centre

PY - 2013/1

Y1 - 2013/1

N2 - Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.

AB - Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.

KW - Genetic diversity

KW - Interrogans

KW - Epidemiology

KW - Relatedness

U2 - 10.1371/journal.pntd.0001954

DO - 10.1371/journal.pntd.0001954

M3 - Article

VL - 7

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2735

IS - 1

M1 - e1954

ER -

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