Skip to content

Research at St Andrews

A transcriptomic approach to the study of osmoregulation in European eel Anguilla anguilla

Research output: Contribution to journalArticle

Author(s)

S Kalujnaia, IS McWilliam, VA Zaguinaiko, AL Feilen, J Nicholson, Neil Hazon, Christopher Paul Cutler, Gordon Cramb

School/Research organisations

Abstract

In euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (FW) and sea water (SW) environments. The European eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. The life cycle of the European eel includes two migratory periods, the second being the migration of FW eels back to the Sargasso Sea for reproduction. Various anatomical and physiological changes allow the successful transition to SW. The aim of this study was to use a microarray approach to screen the osmoregulatory tissues of the eel for changes in gene expression following acclimation to SW. Tissues were sampled from fish at selected intervals over a 5-mo period following FW/SW transfer, and RNA was isolated. Suppressive subtractive hybridization was used for enrichment of differentially expressed genes. Microarrays comprising 6,144 cDNAs from brain, gill, intestine, and kidney libraries were hybridized with appropriate targets and analyzed; 229 differentially expressed clones with unique sequences were identified. These clones represented the sequences for 95 known genes, with the remaining sequences (59%) being unknown. The results of the microarray analysis were validated by quantification of 28 differentially expressed genes by Northern blotting. A number of the differentially expressed genes were already known to be involved in osmoregulation, but the functional roles of many others, not normally associated with ion or water transport, remain to be characterized.

Close

Details

Original languageEnglish
Pages (from-to)385-401
Number of pages17
JournalPhysiological Genomics
Volume31
Issue number3
DOIs
Publication statusPublished - 14 Nov 2007

    Research areas

  • microarray, genes, differential expression, ORNITHINE DECARBOXYLASE-ANTIZYME, APOLIPOPROTEIN MULTIGENE FAMILY, ZEBRAFISH DANIO-RERIO, GENE-EXPRESSION, GROWTH-HORMONE, FRESH-WATER, FISH GILLS, OREOCHROMIS-MOSSAMBICUS, COMPARATIVE PHYSIOLOGY, AMINOPEPTIDASE N/CD13

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Salinity adaptation and gene profiling analysis in the European eel (Anguilla anguilla) using microarray technology

    Kalujnaia, S., McWilliam, I. S., Zaguinaiko, V. A., Feilen, A. L., Nicholson, J., Hazon, N., Cutler, C. P., Balment, R. J., Cossins, A. R., Hughes, M. & Cramb, G., Jun 2007, In : General and Comparative Endocrinology. 152, 2-3, p. 274-280 7 p.

    Research output: Contribution to journalArticle

  2. Cortisol regulates eel (Anguilla anguilla) aquaporin 3 (AQP3) mRNA expression levels in gill

    Cutler, C. P., Phillips, C., Hazon, N. & Cramb, G., Jun 2007, In : General and Comparative Endocrinology. 152, p. 310-313 4 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon

    Bower, N. I. & Johnston, I. A., 29 Sep 2010, In : Physiological Genomics. 42A, 2, p. 114-130 17 p.

    Research output: Contribution to journalArticle

  2. Salmonid genomes have a remarkably expanded akirin family, coexpressed with genes from conserved pathways governing skeletal muscle growth and catabolism

    Macqueen, D. J., Kristjansson, B. K. & Johnston, I. A., Jun 2010, In : Physiological Genomics. 42, 1, p. 134-148 15 p.

    Research output: Contribution to journalArticle

  3. A genomic approach to reveal novel genes associated with myotube formation in the model teleost, Takifugu rubripes

    Fernandes, J. M. O., Mackenzie, M. G., Elgar, G., Suzuki, Y., Watabe, S., Kinghorn, J. R. & Johnston, I. A., 11 Aug 2005, In : Physiological Genomics. 22, 3, p. 327-338 12 p.

    Research output: Contribution to journalArticle

ID: 391985