Skip to content

Research at St Andrews

A transgenic line that reports CSF1R protein expression provides a definitive marker for the mouse mononuclear phagocyte system

Research output: Contribution to journalArticlepeer-review

DOI

Author(s)

Kathleen Grabert, Anuj Sehgal, Katharine M. Irvine, Evi Wollscheid-Lengeling, Derya D. Ozdemir, Jennifer Stables, Garry A. Luke, Martin D. Ryan, Antony Adamson, Neil E. Humphreys, Cheyenne J. Sandrock, Rocio Rojo, Veera A. Verkasalo, Werner Mueller, Peter Hohenstein, Allison R. Pettit, Clare Pridans, David A. Hume

School/Research organisations

Abstract

The proliferation, differentiation, and survival of cells of the mononuclear phagocyte system (MPS; progenitors, monocytes, macrophages, and classical dendritic cells) are controlled by signals from the M-CSF receptor (CSF1R). Cells of the MPS lineage have been identified using numerous surface markers and transgenic reporters, but none is both universal and lineage restricted. In this article, we report the development and characterization of a CSF1R reporter mouse. A FusionRed (FRed) cassette was inserted in-frame with the C terminus of CSF1R, separated by a T2A-cleavable linker. The insertion had no effect of CSF1R expression or function. CSF1R-FRed was expressed in monocytes and macrophages and absent from granulocytes and lymphocytes. In bone marrow, CSF1R-FRed was absent in lineage-negative hematopoietic stem cells, arguing against a direct role for CSF1R in myeloid lineage commitment. It was highly expressed in marrow monocytes and common myeloid progenitors but significantly lower in granulocyte-macrophage progenitors. In sections of bone marrow, CSF1R-FRed was also detected in osteoclasts, CD169+ resident macrophages, and, consistent with previous mRNA analysis, in megakaryocytes. In lymphoid tissues, CSF1R-FRed highlighted diverse MPS populations, including classical dendritic cells. Whole mount imaging of nonlymphoid tissues in mice with combined CSF1R-FRed/Csf1r-EGFP confirmed the restriction of CSF1R expression to MPS cells. The two markers highlight the remarkable abundance and regular distribution of tissue MPS cells, including novel macrophage populations within tendon and skeletal muscle and underlying the mesothelial/serosal/capsular surfaces of every major organ. The CSF1R-FRed mouse provides a novel reporter with exquisite specificity for cells of the MPS.
Close

Details

Original languageEnglish
JournalThe Journal of Immunology
Volume205
Issue number11
Early online date23 Nov 2020
DOIs
Publication statusPublished - 1 Dec 2020

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Expression and immunogenicity of secreted forms of bovine ephemeral fever virus glycoproteins applied to subunit vaccine development

    Lo, Y-T., Tulloch, F., Wu, H-C., Luke, G. A., Ryan, M. D. & Chu, C-Y., 3 Mar 2021, In: Journal of Applied Microbiology. Early View

    Research output: Contribution to journalArticlepeer-review

  2. Translation of Viral Proteins

    Ryan, M. D. & Luke, G. A., 2021, Encyclopedia of Virology: The Virus as a Concept - Fundamentals of Virology. Bamford, D. & Zuckerman, M. (eds.). 4 ed. New York: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Vol. 1. p. 444-459 15 p.

    Research output: Chapter in Book/Report/Conference proceedingChapter

  3. Therapeutic applications of the 'NPGP' family of viral 2As

    Luke, G. A. & Ryan, M. D., Nov 2018, In: Reviews in Medical Virology. 28, 6, 12 p., e2001.

    Research output: Contribution to journalReview articlepeer-review

  4. Problems in FMD eradication: a way forward?

    Tulloch, F., Luke, G. A. & Ryan, M. D., 5 Jul 2018, In: Journal of Veterinary Medicine and Research. 5, 5, 6 p., 1140.

    Research output: Contribution to journalReview articlepeer-review

  5. Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

    Luke, G. A. & Ryan, M. D., 2018, Reporter Gene Assays: Methods and Protocols. Damoiseaux, R. & Hasson, S. (eds.). New York: Humana Press/Springer, Vol. 1755. p. 31-48

    Research output: Chapter in Book/Report/Conference proceedingChapter

ID: 271681583

Top