Skip to content

Research at St Andrews

A virulent strain of Deformed Wing Virus (DWV) of Honeybees (Apis mellifera) prevails after Varroa destructor-mediated, or in vitro, transmission

Research output: Contribution to journalArticle

Author(s)

Eugene V. Ryabov, Graham R. Wood, Jessica M. Fannon, Jonathan D. Moore, James C. Bull, Dave Chandler, Andrew Mead, Nigel Burroughs, David J. Evans

School/Research organisations

Abstract

The globally distributed ectoparasite Varroa destructor is a vector for viral pathogens of the Western honeybee (Apis mellifera), in particular the Iflavirus Deformed Wing Virus (DWV). In the absence of Varroa low levels DWV occur, generally causing asymptomatic infections. Conversely, Varroa-infested colonies show markedly elevated virus levels, increased overwintering colony losses, with impairment of pupal development and symptomatic workers. To determine whether changes in the virus population were due Varroa amplifying and introducing virulent virus strains and/or suppressing the host immune responses, we exposed Varroa-naive larvae to oral and Varroa-transmitted DWV. We monitored virus levels and diversity in developing pupae and associated Varroa, the resulting RNAi response and transcriptome changes in the host. Exposed pupae were stratified by Varroa association (presence/absence) and virus levels (low/high) into three groups. Varroa-free pupae all exhibited low levels of a highly diverse DWV population, with those exposed per os (group NV) exhibiting changes in the population composition. Varroa-associated pupae exhibited either low levels of a diverse DWV population (group VL) or high levels of a near-clonal virulent variant of DWV (group VH). These groups and unexposed controls (C) could be also discriminated by principal component analysis of the transcriptome changes observed, which included several genes involved in development and the immune response. All Varroa tested contained a diverse replicating DWV population implying the virulent variant present in group VH, and predominating in RNA-seq analysis of temporally and geographically separate Varroa-infested colonies, was selected upon transmission from Varroa, a conclusion supported by direct injection of pupae in vitro with mixed virus populations. Identification of a virulent variant of DWV, the role of Varroa in its transmission and the resulting host transcriptome changes furthers our understanding of this important viral pathogen of honeybees.

Close

Details

Original languageEnglish
Article numbere1004230
Number of pages21
JournalPLoS Pathogens
Volume10
Issue number6
DOIs
StatePublished - 26 Jun 2014

    Research areas

  • Colony collapse disorder, Picorna-like virus, Drosophila hematopoiesis, Antiviral defense, Microarray data

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Mechanisms and consequences of positive-strand RNA virus recombination

    Bentley, K. & Evans, D. J. 1 Oct 2018 In : Journal of General Virology. 99, 10, p. 1345-1356

    Research output: Contribution to journalReview article

  2. Energy limitation of cyanophage development: implications for marine carbon cycling

    Puxty, R. J., Evans, D. J., Millard, A. D. & Scanlan, D. J. May 2018 In : ISME Journal. 12, 5, p. 1273-1286 14 p.

    Research output: Contribution to journalArticle

  3. CpG and UpA dinucleotides in both coding and non-coding regions of echovirus 7 inhibit replication initiation post-entry

    Fros, J. J., Dietrich, I., Alshaikhahmed, K., Passchier, T. C., Evans, D. J. & Simmonds, P. 29 Sep 2017 In : eLife. 6, 29 p., e29112

    Research output: Contribution to journalArticle

  4. Biochemical and genetic analysis of the role of the viral polymerase in enterovirus recombination

    Woodman, A., Arnold, J., Cameron, C. & Evans, D. J. 19 Aug 2016 In : Nucleic Acids Research. 44, 14, p. 6883-6895

    Research output: Contribution to journalArticle

Related by journal

  1. Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei

    Tonini, M. L., Peña-Diaz, P., Haindrich, A. C., Basu, S., Kriegová, E., Pierik, A. J., Lill, R., MacNeill, S. A., Smith, T. K. & Lukeš, J. 22 Oct 2018 In : PLoS Pathogens. 14, 10, 31 p., e1007326

    Research output: Contribution to journalArticle

  2. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase

    Liu, X-J., Yang, B., Huang, S-N., Wu, C-C., Li, X-J., Cheng, S., Jiang, X., Hu, F., Ming, Y-Z., Nevels, M. M., Britt, W. J., Rayner, S., Tang, Q., Zeng, W-B., Zhao, F. & Luo, M-H. 27 Jul 2017 In : PLoS Pathogens. 13, 7, 28 p., e1006542

    Research output: Contribution to journalArticle

  3. Human cytomegalovirus immediate-early 1 protein rewires upstream STAT3 to downstream STAT1 signaling switching an IL6-type to an IFNγ-like response

    Harwardt, T., Lukas, S., Zenger, M., Reitberger, T., Danzer, D., Übner, T., Munday, D. C., Nevels, M. & Paulus, C. 7 Jul 2016 In : PLoS Pathogens. 12, 7, 39 p., e1005748

    Research output: Contribution to journalArticle

  4. Vaccinia virus immunomodulator A46: a lipid and protein-binding scaffold for sequestering host TIR-domain proteins

    Fedosyuk, S., Bezerra, G. A., Radakovics, K., Smith, T. K., Sammito, M., Bobik, N., Round, A., Ten Eyck, L. F., Djinović-Carugo, K., Usón, I. & Skern, T. 14 Dec 2016 In : PLoS Pathogens. 12, 12, 24 p., e1006079

    Research output: Contribution to journalArticle

  5. NEDDylation is essential for Kaposi’s Sarcoma-associated herpesvirus latency and lytic reactivation and represents a novel anti-KSHV target

    Hughes, D. J., Wood, J. J., Jackson, B. R., Baquero-Pérez, B. & Whitehouse, A. 20 Mar 2015 In : PLoS Pathogens. 11, 3, 26 p., e1004771

    Research output: Contribution to journalArticle

Related by journal

  1. PLoS Pathogens (Journal)

    Smith, T. K. (Member of editorial board)
    2015 → …

    Activity: Publication peer-review and editorial workEditor of research journal

ID: 211241515