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ABIN-3: a molecular basis for species divergence in interleukin-10-induced anti-inflammatory actions

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Author(s)

Brian K. Weaver, Erwin Bohn, Barbi A. Judd, M. Pilar Gil, Robert D. Schreiber

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Abstract

Whereas interleukin-10 (IL-10) is an anti-inflammatory cytokine known to regulate macrophage activation, its full mechanism of action remains incompletely defined. In a screen to identify novel IL-10-induced genes, we cloned the mouse ortholog of human ABIN-3 (also termed LIND). ABIN-3 expression was induced selectively by IL-10 in both mouse and human mononuclear phagocytes coordinately undergoing proinflammatory responses. In contrast to the previously characterized ABINs, mouse ABIN-3 was incapable of inhibiting NF-kappa B activation by proinflammatory stimuli. Generation and analysis of ABIN-3-null mice demonstrated that ABIN-3 is unnecessary for the anti-inflammatory effects of IL-10 as well as for proper negative regulation of NF-kappa B. Conversely, human ABIN-3 was capable of inhibiting NF-kappa B activation in response to signaling from Toll-like receptor, IL-1, and tumor necrosis factor. Enforced expression of human ABIN-3 in human monocytic cells suppressed the cytoplasmic degradation of I kappa B alpha, the activation of NF-kappa B, and the induction of proinflammatory genes. Comparative sequence analyses revealed that mouse ABIN-3 lacks a complete ABIN homology domain, which was required for the functional activity of human ABIN-3. ABIN-3 is, thus, an IL-10-induced gene product capable of attenuating NF-kappa B in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions.

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Details

Original languageEnglish
Pages (from-to)4603-4616
Number of pages14
JournalMolecular and Cellular Biology
Volume27
Issue number13
DOIs
Publication statusPublished - Jul 2007

    Research areas

  • NF-KAPPA-B, TNF-ALPHA PRODUCTION, SIGNAL-TRANSDUCTION PATHWAY, MESSENGER-RNA EXPRESSION, NITRIC-OXIDE PRODUCTION, HUMAN MONOCYTES, HUMAN MACROPHAGES, GENE-EXPRESSION, INTERLEUKIN-10-DEFICIENT MICE, A20-BINDING INHIBITOR

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