Skip to content

Research at St Andrews

Absence of protein A expression is associated with higher capsule production in staphylococcal isolates

Research output: Contribution to journalArticlepeer-review

Author(s)

Tarcisio Brignoli, Andrea G. O. Manetti, Roberto Rosini, Andreas F. Haag, Vincenzo Scarlato, Fabio Bagnoli, Isabel Delany

School/Research organisations

Abstract

Staphylococcus aureus is a major human pathogen, and a leading cause of soft tissue and blood stream infections. One of the causes of its success as a pathogen is the peculiar array of immune evasion factors through which the bacterium avoids host defenses, where the staphylococcal protein A (SpA) plays a major role thanks to its IgG binding activities. Moreover, SpA has recently been proposed as a promising vaccine antigen. In this study, we evaluated the expression of SpA in a collection of staphylococcal strains, about 7% of which did not express SpA (SpA- strains), despite the presence of the gene. By a comparative genomic analysis, we identified that a mutation in the spa 5′ UTR sequence affecting the RBS is responsible for the loss of SpA in a subset of SpA- strains. Using a high-throughput qRT-PCR approach on a selected panel of virulence-related genes, we identified that the SpA- phenotype is associated with lower spa transcript levels and increased expression and production of capsule as well as other changes in the transcription of several key virulence factors. Our data suggest that the SpA- phenotype has occurred in geographically distinct strains through different molecular mechanisms including both mutation, leading likely to translation alterations, and transcriptional deregulation. Furthermore, we provide evidence that SpA- strains are highly susceptible to phagocytic uptake mediated by anti-capsule antibodies. These data suggest that S. aureus may alter its virulence factor expression pattern as an adaptation to the host or environment. Vaccination strategies targeting both SpA and capsule could therefore result in broader coverage against staphylococcal isolates than SpA alone.
Close

Details

Original languageEnglish
Article number863
Number of pages15
JournalFrontiers in Microbiology
Volume10
DOIs
Publication statusPublished - 10 May 2019

    Research areas

  • Staphylococcus aureus, Protein A, Capsule, Transcription profiling, Vaccines

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Bacterial chromosomal mobility via lateral transduction exceeds that of classical mobile genetic elements

    Humphrey, S., Fillol-Salom, A., Quiles-Puchalt, N., Ibarra-Chávez, R., Haag, A. F., Chen, J. & Penadés, J. R., 8 Nov 2021, In: Nature Communications. 12, 13 p., 6509.

    Research output: Contribution to journalArticlepeer-review

  2. A regulatory cascade controls Staphylococcus aureus pathogenicity island activation

    Haag, A. F., Podkowik, M., Ibarra-Chávez, R., Gallego del Sol, F., Ram, G., Chen, J., Marina, A., Novick, R. P. & Penadés, J. R., 13 Sep 2021, In: Nature Microbiology. 6, 10, p. 1300-1308 9 p.

    Research output: Contribution to journalArticlepeer-review

  3. Radical genome remodelling accompanied the emergence of a novel host-restricted bacterial pathogen

    Yebra, G., Haag, A. F., Neamah, M. M., Wee, B. A., Richardson, E. J., Horcajo, P., Granneman, S., Tormo-Más, M. Á., de la Fuente, R., Fitzgerald, J. R. & Penadés, J. R., 20 May 2021, In: PLoS Pathogens. 17, 5, 23 p., e1009606.

    Research output: Contribution to journalArticlepeer-review

  4. Systematic reconstruction of the complete two-component sensorial network in Staphylococcus aureus

    Rapun-Araiz, B., Haag, A. F., Cesare, V. D., Gil, C., Dorado-Morales, P., Penades, J. R., Lasa, I. & Methe, B. (ed.), 25 Aug 2020, In: mSystems. 5, 4, 16 p., e00511-20.

    Research output: Contribution to journalArticlepeer-review

  5. The impact of two-component sensorial network in staphylococcal speciation

    Rapun-Araiz, B., Haag, A. F., Solano, C. & Lasa, I., Jun 2020, In: Current Opinion in Microbiology. 55, p. 40-47 8 p.

    Research output: Contribution to journalReview articlepeer-review

Related by journal

  1. Frontiers in Microbiology (Journal)

    David John Hughes (Reviewer)

    Jul 2015 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. Frontiers in Microbiology (Journal)

    Michael Martin Nevels (Member of editorial board)

    May 2010 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

Related by journal

  1. Genomic analysis of global Staphylococcus argenteus strains reveals distinct lineages with differing virulence and antibiotic resistance gene content

    Goswami, C., Fox, S., Holden, M., Leanord, A. & Evans, T. J., 2 Dec 2021, In: Frontiers in Microbiology. 12, 18 p., 795173.

    Research output: Contribution to journalArticlepeer-review

  2. Bacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africa

    Osei-Wusu, S., Morgan, P., Asare, P., Adams, G., Musah, A. B., Siam, I. M., Gillespie, S. H., Sabiiti, W. & Yeboah-Manu, D., 27 Oct 2021, (E-pub ahead of print) In: Frontiers in Microbiology. 12, 719531.

    Research output: Contribution to journalArticlepeer-review

  3. Chemical dispersant enhances microbial exopolymer (EPS) production and formation of marine oil/dispersant snow in surface waters of the subarctic northeast Atlantic

    Suja, L. D., Chen, X., Summers, S., Paterson, D. M. & Gutierrez, T., 20 Mar 2019, In: Frontiers in Microbiology. 10, 13 p., 553.

    Research output: Contribution to journalArticlepeer-review

  4. An ionic limit to life in the deep subsurface

    Payler, S. J., Biddle, J. F., Sherwood Lollar, B., Fox-Powell, M. G., Edwards, T., Ngwenya, B. T., Paling, S. M. & Cockell, C. S., 12 Mar 2019, In: Frontiers in Microbiology. 10, 15 p., 00426.

    Research output: Contribution to journalArticlepeer-review

ID: 276905823

Top