Skip to content

Research at St Andrews

Albumin as a zinc carrier: properties of its high-affinity zinc-binding site

Research output: Contribution to journalArticle

DOI

Author(s)

J Lu, Alan James Stewart, PJ Sadler, TJT Pinheiro, CA Blindauer

School/Research organisations

Abstract

Although details of the molecular mechanisms for the uptake of the essential nutrient zinc into the bloodstream and its subsequent delivery to zinc-requiring organs and cells are poorly understood, it is clear that in vertebrates the majority of plasma zinc (9-14 microM; approx. 75-85%) is bound to serum albumin, constituting part of the so-called exchangeable pool. The binding of metal ions to serum albumins has been the subject of decades of studies, employing a multitude of techniques, but only recently has the identity and putative structure of the major zinc site on albumin been reported. Intriguingly, this site is located at the interface between two domains, and involves two residues from each of domains I and II. Comparisons of X-ray crystal structures of free and fatty-acid bound human serum albumin suggest that zinc binding to this site and fatty acid binding to one of the five major sites may be interdependent. Interactive binding of zinc and long-chain fatty acids to albumin may therefore have physiological implications.
Close

Details

Original languageEnglish
Pages (from-to)1317-1321
Number of pages5
JournalBiochemical Society Transactions
Volume36
Issue number6
DOIs
Publication statusPublished - Dec 2008

    Research areas

  • Albumin, Fatty acid, Metal specificity, Serum, Zinc trafficking

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Reduced plasma magnesium levels in type-1 diabetes associate with prothrombotic changes in fibrin clotting and fibrinolysis

    Sobczak, A. I. S., Phoenix, F. A., Pitt, S. J., Ajjan, R. A. & Stewart, A. J., 11 Nov 2019, (Accepted/In press) In : Thrombosis and Haemostasis.

    Research output: Contribution to journalArticle

  2. A metalloproteomic analysis of interactions between plasma proteins and zinc: elevated fatty acid levels affect zinc distribution

    Coverdale, J. P. C., Barnett, J. P., Adamu, A. H., Griffiths, E. J., Stewart, A. J. & Blindauer, C. A., 1 Nov 2019, In : Metallomics. 11, 11, p. 1805-1819 15 p.

    Research output: Contribution to journalArticle

  3. Plasma non-esterified fatty acids contribute to increased coagulability in type-2 diabetes through altered plasma zinc speciation

    Sobczak, A. I. S., Katundu, K. G. H., Phoenix, F. A., Khazaipoul, S., Yu, R., Lampiao, F., Stefanowicz, F., Blindauer, C. A., Pitt, S. J., Smith, T. K., Ajjan, R. A. & Stewart, A. J., 28 Aug 2019, In : biorxiv. 38 p.

    Research output: Contribution to journalArticle

  4. Changes in plasma free fatty acids associated with type-2 diabetes

    Sobczak, A. I. S., Blindauer, C. A. & Stewart, A. J., 28 Aug 2019, In : Nutrients. 11, 9, 42 p., 2022.

    Research output: Contribution to journalReview article

  5. Sub-micromolar pulse dipolar EPR spectroscopy reveals increasing CuII-labelling of double-histidine motifs with lower temperature

    Wort, J. L., Ackermann, K., Giannoulis, A., Stewart, A. J., Norman, D. G. & Bode, B. E., 19 Aug 2019, In : Angewandte Chemie - International Edition. 58, 34, p. 11681-11685 5 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Examining a new role for zinc in regulating calcium release in cardiac muscle

    Pitt, S. J. & Stewart, A. J., 1 Jun 2015, In : Biochemical Society Transactions. 43, 3, p. 359-363

    Research output: Contribution to journalArticle

  2. Novel flavonoids as anti-cancer agents: mechanisms of action and promise for their potential application in breast cancer.

    Martinez-Perez, C., Ward, C., Cook, G., Mullen, P., D, M., Harrison, D. & Langdon, S., 1 Aug 2014, In : Biochemical Society Transactions. 42, 4, p. 1017-1023 7 p.

    Research output: Contribution to journalArticle

  3. Hot and crispy: CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus

    Zhang, J. & White, M. F., Dec 2013, In : Biochemical Society Transactions. 41, 6, p. 1422-1426 5 p.

    Research output: Contribution to journalArticle

  4. Regulation of the cardiac Na+ pump by palmitoylation of its catalytic and regulatory subunits

    Howie, J., Tulloch, L. B., Shattock, M. J. & Fuller, W., Feb 2013, In : Biochemical Society Transactions. 41, 1, p. 95-100 6 p.

    Research output: Contribution to journalArticle

  5. Small nuclear RNAs and mRNAs: linking RNA processing and transport to spinal muscular atrophy

    Sleeman, J., Aug 2013, In : Biochemical Society Transactions. 41, 4, p. 871-875 5 p.

    Research output: Contribution to journalArticle

ID: 434209

Top