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All-optical assay to study biological neural networks

Research output: Contribution to journalArticle

Author(s)

Wardiya Afshar Saber, Federico M. Gasparoli, Marjet Gabrielle Dirks, Frank J. Gunn-Moore, Maciej Antkowiak

School/Research organisations

Abstract

We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.
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Details

Original languageEnglish
Article number451
Number of pages12
JournalFrontiers in Neuroscience
Volume12
DOIs
Publication statusPublished - 5 Jul 2018

    Research areas

  • All-optical assay, Optogenetics, Calcium imaging, Neural networks, Connectivity, Neurons, Lentiviral transduction

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