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An unusual flavin-dependent halogenase from the metagenome of the marine sponge Theonella swinhoei WA

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Author(s)

Duncan R. M. Smith, Agustinus R. Uria, Eric J. N. Helfrich, Daniela Milbredt, Karl-Heinz van Pée, Jörn Piel, Rebecca J. M. Goss

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Abstract

Uncultured bacteria from sponges have been demonstrated to be responsible for the generation of many potent, bioactive natural products including halogenated metabolites.1 The identification of gene clusters from the metagenomes of such bacterial communities enables the discovery of enzymes that mediate new and useful chemistries and allows insight to be gained into the biogenesis of potentially pharmacologically important natural products. Here we report a new pathway to the keramamides (krm); the first functional evidence for the existence of a distinct producer in the Theonella swinhoei WA chemotype is revealed, and a key enzyme on the pathway, a unique flavin dependent halogenase with a broad substrate specificity, and with potential as a useful new biocatalytic tool is described.
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Original languageEnglish
Pages (from-to)1281-1287
Number of pages7
JournalACS Chemical Biology
Volume12
Issue number5
Early online date15 Feb 2017
DOIs
StatePublished - 19 May 2017

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