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At the transition from invertebrates to vertebrates a novel GnRH-like peptide emerges in amphioxus

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Author(s)

Graeme Roch, Javier Tello, Nancy Sherwood

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Abstract

Gonadotropin-releasing hormone (GnRH) is a critical reproductive regulator in vertebrates. Homologous peptides are also found in invertebrates, with a variety of characterized functions. In the amphioxus, an invertebrate that provides the best model for the transition to vertebrates, four GnRH receptors were previously described but their native ligands were not identified. Using a more sensitive search methodology with hidden Markov models (HMMs), we identified the first GnRH-like peptide confirmed in the amphioxus Branchistoma floridae. This peptide specifically activated one of the four GnRH receptors. Although the primary structure of this peptide was divergent from any previously isolated GnRH peptide, the minimal conserved residues found in all other GnRH superfamily members were retained. The peptide was immunolocalized in proximity of the central canal of the anterior nerve cord, a region where other neuropeptides and receptors have been found. Additionally, the amphioxus GnRH-like gene was positioned in a locus surrounded by syntenic homologs of the human GnRH paralogon. The amphioxus GnRH-like peptide, with its distinct primary structure, activated a receptor with equal potency to multiple ligands that span the GnRH superfamily.
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Original languageEnglish
Pages (from-to)765-778
JournalMolecular Biology and Evolution
Volume31
Issue number4
Early online date20 Dec 2013
DOIs
Publication statusPublished - 20 Dec 2013

    Research areas

  • Hormone evolution , Adipokinetic hormone, Corazonin, Amphioxus genome, GnRH superfamily, Receptor evolution

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