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Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6

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Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6. / Elfick, Alistair; Rischitor, Grigore; Mouras, Rabah; Azfer, Asim; Lungaro, Lisa; Uhlarz, Marc; Herrmannsdörfer, Thomas; Lucocq, John Milton; Gamal, Wesam; Bagnaninchi, Pierre; Semple, Scott; Salter, Donald M.

In: Scientific Reports, Vol. 7, 39755, 04.01.2017.

Research output: Contribution to journalArticlepeer-review

Harvard

Elfick, A, Rischitor, G, Mouras, R, Azfer, A, Lungaro, L, Uhlarz, M, Herrmannsdörfer, T, Lucocq, JM, Gamal, W, Bagnaninchi, P, Semple, S & Salter, DM 2017, 'Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6', Scientific Reports, vol. 7, 39755. https://doi.org/10.1038/srep39755

APA

Elfick, A., Rischitor, G., Mouras, R., Azfer, A., Lungaro, L., Uhlarz, M., Herrmannsdörfer, T., Lucocq, J. M., Gamal, W., Bagnaninchi, P., Semple, S., & Salter, D. M. (2017). Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6. Scientific Reports, 7, [39755]. https://doi.org/10.1038/srep39755

Vancouver

Elfick A, Rischitor G, Mouras R, Azfer A, Lungaro L, Uhlarz M et al. Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6. Scientific Reports. 2017 Jan 4;7. 39755. https://doi.org/10.1038/srep39755

Author

Elfick, Alistair ; Rischitor, Grigore ; Mouras, Rabah ; Azfer, Asim ; Lungaro, Lisa ; Uhlarz, Marc ; Herrmannsdörfer, Thomas ; Lucocq, John Milton ; Gamal, Wesam ; Bagnaninchi, Pierre ; Semple, Scott ; Salter, Donald M. / Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6. In: Scientific Reports. 2017 ; Vol. 7.

Bibtex - Download

@article{377c65d8168e4c26baf85427b5822c3e,
title = "Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6",
abstract = "The use of stem cells to support tissue repair is facilitated by loading of thetherapeutic cells with magnetic nanoparticles (MNPs) enabling magnetic tracking and targeting. Current methods for magnetizing cells use artificial MNPs and have disadvantages of variable uptake, cellular cytotoxicity and loss of nanoparticles on cell division. Here we demonstrate a transgenic approach to magnetize human mesenchymal stem cells (MSCs). MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesises single-magnetic domain crystals which are incorporated into magnetosomes. Following transfection of MSCs with the mms6 gene there is bioassimilatedsynthesis of intracytoplasmic magnetic nanoparticles which can beimaged by MR and which have no deleterious effects on cell proliferation, migration or differentiation. The assimilation of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompatible, alternative to exogenous administration of MNPs.",
author = "Alistair Elfick and Grigore Rischitor and Rabah Mouras and Asim Azfer and Lisa Lungaro and Marc Uhlarz and Thomas Herrmannsd{\"o}rfer and Lucocq, {John Milton} and Wesam Gamal and Pierre Bagnaninchi and Scott Semple and Salter, {Donald M.}",
note = "This work was funded under the FP7 EU project “MAGISTER”. Grant Number: NMP3-LA-2008-214685 and additionally supported through a MRC Confidence in Concept scheme award to the University of Edinburgh. ",
year = "2017",
month = jan,
day = "4",
doi = "10.1038/srep39755",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature publishing group",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6

AU - Elfick, Alistair

AU - Rischitor, Grigore

AU - Mouras, Rabah

AU - Azfer, Asim

AU - Lungaro, Lisa

AU - Uhlarz, Marc

AU - Herrmannsdörfer, Thomas

AU - Lucocq, John Milton

AU - Gamal, Wesam

AU - Bagnaninchi, Pierre

AU - Semple, Scott

AU - Salter, Donald M.

N1 - This work was funded under the FP7 EU project “MAGISTER”. Grant Number: NMP3-LA-2008-214685 and additionally supported through a MRC Confidence in Concept scheme award to the University of Edinburgh.

PY - 2017/1/4

Y1 - 2017/1/4

N2 - The use of stem cells to support tissue repair is facilitated by loading of thetherapeutic cells with magnetic nanoparticles (MNPs) enabling magnetic tracking and targeting. Current methods for magnetizing cells use artificial MNPs and have disadvantages of variable uptake, cellular cytotoxicity and loss of nanoparticles on cell division. Here we demonstrate a transgenic approach to magnetize human mesenchymal stem cells (MSCs). MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesises single-magnetic domain crystals which are incorporated into magnetosomes. Following transfection of MSCs with the mms6 gene there is bioassimilatedsynthesis of intracytoplasmic magnetic nanoparticles which can beimaged by MR and which have no deleterious effects on cell proliferation, migration or differentiation. The assimilation of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompatible, alternative to exogenous administration of MNPs.

AB - The use of stem cells to support tissue repair is facilitated by loading of thetherapeutic cells with magnetic nanoparticles (MNPs) enabling magnetic tracking and targeting. Current methods for magnetizing cells use artificial MNPs and have disadvantages of variable uptake, cellular cytotoxicity and loss of nanoparticles on cell division. Here we demonstrate a transgenic approach to magnetize human mesenchymal stem cells (MSCs). MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesises single-magnetic domain crystals which are incorporated into magnetosomes. Following transfection of MSCs with the mms6 gene there is bioassimilatedsynthesis of intracytoplasmic magnetic nanoparticles which can beimaged by MR and which have no deleterious effects on cell proliferation, migration or differentiation. The assimilation of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompatible, alternative to exogenous administration of MNPs.

U2 - 10.1038/srep39755

DO - 10.1038/srep39755

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 39755

ER -

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