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Cell patterning on photolithographically defined parylene-C: SiO2 substrates

Research output: Contribution to journalArticlepeer-review

DOI

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Cell patterning on photolithographically defined parylene-C : SiO2 substrates. / Hughes, Mark A.; Brennan, Paul M.; Bunting, Andrew S.; Shipston, Mike J.; Murray, Alan F.

In: Journal of Visualized Experiments, No. 85, e50929, 07.03.2014.

Research output: Contribution to journalArticlepeer-review

Harvard

Hughes, MA, Brennan, PM, Bunting, AS, Shipston, MJ & Murray, AF 2014, 'Cell patterning on photolithographically defined parylene-C: SiO2 substrates', Journal of Visualized Experiments, no. 85, e50929. https://doi.org/10.3791/50929

APA

Hughes, M. A., Brennan, P. M., Bunting, A. S., Shipston, M. J., & Murray, A. F. (2014). Cell patterning on photolithographically defined parylene-C: SiO2 substrates. Journal of Visualized Experiments, (85), [e50929]. https://doi.org/10.3791/50929

Vancouver

Hughes MA, Brennan PM, Bunting AS, Shipston MJ, Murray AF. Cell patterning on photolithographically defined parylene-C: SiO2 substrates. Journal of Visualized Experiments. 2014 Mar 7;(85). e50929. https://doi.org/10.3791/50929

Author

Hughes, Mark A. ; Brennan, Paul M. ; Bunting, Andrew S. ; Shipston, Mike J. ; Murray, Alan F. / Cell patterning on photolithographically defined parylene-C : SiO2 substrates. In: Journal of Visualized Experiments. 2014 ; No. 85.

Bibtex - Download

@article{41fe72a50d1645f191940d8e41893cd5,
title = "Cell patterning on photolithographically defined parylene-C: SiO2 substrates",
abstract = "Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain central aspects of cellular physiology. To easily combine cell patterning with Multi-Electrode Arrays (MEAs) and silicon-based 'lab on a chip' technologies, a microfabrication-compatible protocol is required. We describe a method that utilizes deposition of the polymer parylene-C on SiO2 wafers. Photolithography enables accurate and reliable patterning of parylene-C at micron-level resolution. Subsequent activation by immersion in fetal bovine serum (or another specific activation solution) results in a substrate in which cultured cells adhere to, or are repulsed by, parylene or SiO2 regions respectively. This technique has allowed patterning of a broad range of cell types (including primary murine hippocampal cells, HEK 293 cell line, human neuron-like teratocarcinoma cell line, primary murine cerebellar granule cells, and primary human glioma-derived stem-like cells). Interestingly, however, the platform is not universal; reflecting the importance of cell-specific adhesion molecules. This cell patterning process is cost effective, reliable, and importantly can be incorporated into standard microfabrication (chip manufacturing) protocols, paving the way for integration of microelectronic technology.",
keywords = "Bioengineering, Biomedical and Dental Materials, Cell adhesion, Cell Adhesion, Cell Patterning, Cell Surface, Issue 85, Parylene-C, Photolithography, Polymers, Receptors, Silicon dioxide",
author = "Hughes, {Mark A.} and Brennan, {Paul M.} and Bunting, {Andrew S.} and Shipston, {Mike J.} and Murray, {Alan F.}",
year = "2014",
month = mar,
day = "7",
doi = "10.3791/50929",
language = "English",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "85",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cell patterning on photolithographically defined parylene-C

T2 - SiO2 substrates

AU - Hughes, Mark A.

AU - Brennan, Paul M.

AU - Bunting, Andrew S.

AU - Shipston, Mike J.

AU - Murray, Alan F.

PY - 2014/3/7

Y1 - 2014/3/7

N2 - Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain central aspects of cellular physiology. To easily combine cell patterning with Multi-Electrode Arrays (MEAs) and silicon-based 'lab on a chip' technologies, a microfabrication-compatible protocol is required. We describe a method that utilizes deposition of the polymer parylene-C on SiO2 wafers. Photolithography enables accurate and reliable patterning of parylene-C at micron-level resolution. Subsequent activation by immersion in fetal bovine serum (or another specific activation solution) results in a substrate in which cultured cells adhere to, or are repulsed by, parylene or SiO2 regions respectively. This technique has allowed patterning of a broad range of cell types (including primary murine hippocampal cells, HEK 293 cell line, human neuron-like teratocarcinoma cell line, primary murine cerebellar granule cells, and primary human glioma-derived stem-like cells). Interestingly, however, the platform is not universal; reflecting the importance of cell-specific adhesion molecules. This cell patterning process is cost effective, reliable, and importantly can be incorporated into standard microfabrication (chip manufacturing) protocols, paving the way for integration of microelectronic technology.

AB - Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain central aspects of cellular physiology. To easily combine cell patterning with Multi-Electrode Arrays (MEAs) and silicon-based 'lab on a chip' technologies, a microfabrication-compatible protocol is required. We describe a method that utilizes deposition of the polymer parylene-C on SiO2 wafers. Photolithography enables accurate and reliable patterning of parylene-C at micron-level resolution. Subsequent activation by immersion in fetal bovine serum (or another specific activation solution) results in a substrate in which cultured cells adhere to, or are repulsed by, parylene or SiO2 regions respectively. This technique has allowed patterning of a broad range of cell types (including primary murine hippocampal cells, HEK 293 cell line, human neuron-like teratocarcinoma cell line, primary murine cerebellar granule cells, and primary human glioma-derived stem-like cells). Interestingly, however, the platform is not universal; reflecting the importance of cell-specific adhesion molecules. This cell patterning process is cost effective, reliable, and importantly can be incorporated into standard microfabrication (chip manufacturing) protocols, paving the way for integration of microelectronic technology.

KW - Bioengineering

KW - Biomedical and Dental Materials

KW - Cell adhesion

KW - Cell Adhesion

KW - Cell Patterning

KW - Cell Surface

KW - Issue 85

KW - Parylene-C

KW - Photolithography

KW - Polymers

KW - Receptors

KW - Silicon dioxide

UR - http://www.scopus.com/inward/record.url?scp=84940215510&partnerID=8YFLogxK

U2 - 10.3791/50929

DO - 10.3791/50929

M3 - Article

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 85

M1 - e50929

ER -

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