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Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells

Research output: Contribution to journalArticlepeer-review

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Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. / Tucker, John Barry; Mogensen, MM; Mackie, JB; Doxsey, SJ; 1, other.

In: Cell Motility and the Cytoskeleton, Vol. 36, 1997, p. 276-290.

Research output: Contribution to journalArticlepeer-review

Harvard

Tucker, JB, Mogensen, MM, Mackie, JB, Doxsey, SJ & 1, O 1997, 'Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells', Cell Motility and the Cytoskeleton, vol. 36, pp. 276-290.

APA

Tucker, J. B., Mogensen, MM., Mackie, JB., Doxsey, SJ., & 1, O. (1997). Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. Cell Motility and the Cytoskeleton, 36, 276-290.

Vancouver

Tucker JB, Mogensen MM, Mackie JB, Doxsey SJ, 1 O. Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. Cell Motility and the Cytoskeleton. 1997;36:276-290.

Author

Tucker, John Barry ; Mogensen, MM ; Mackie, JB ; Doxsey, SJ ; 1, other. / Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. In: Cell Motility and the Cytoskeleton. 1997 ; Vol. 36. pp. 276-290.

Bibtex - Download

@article{3aba4053a0b84ba48252cc2ffe3a9c16,
title = "Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells",
abstract = "This report provides evidence for two functionally and spatially distinct centrosomal domains in certain mouse cochlear epithelial cells. The vast majority of microtubules elongate from sites associated with the apical cell surface in these cells rather than from pericentriolar material surrounding the immediate environs of their apically situated centrioles. The distribution of gamma-tubulin and pericentrin at cell apices has been examined while microtubule nucleation is progressing because these centrosomal proteins are believed to be essential for microtubule nucleation.Antibodies to both proteins bind to pericentriolar regions but no binding has been detected at the apical cell surface-associated sites where the ends of thousands of recently nucleated microtubules are concentrated. Sparse transient microtubule populations can be detected between pericentriolar regions and surface sites while microtubule assembly advances. A procedure apparently operates in which the pericentriolar region functions as a microtubule-nucleating domain and the cell surface-associated sites operate as docking domains which capture the minus ends of microtubules that migrate to them shortly after nucleation.Docking domains may include some components of the pericentriolar material that have been relocated at the cell apex. A docking element hypothesis for centrosomal control of minus end positioning and dynamics in animal cells generally is proposed.This investigation has also shown that the concentration of gamma-tubulin and pericentrin around centrioles differs spatially and quantitatively in ways that are characteristic for the four cell types studied. Some of these characteristics can be related to differences in control of microtubule number and positioning. (C) 1997 Wiley-Liss, Inc.",
keywords = "centrosomal organization, microtubule-nucleation, mouse cochlea, ORGANIZING CENTERS, MAMMALIAN-CELLS, SERTOLI CELLS, ORGANIZATION, DROSOPHILA, DYNAMICS, POLARITY, MITOSIS, REORGANIZATION, RECONSTITUTION",
author = "Tucker, {John Barry} and MM Mogensen and JB Mackie and SJ Doxsey and other 1",
year = "1997",
language = "English",
volume = "36",
pages = "276--290",
journal = "Cell Motility and the Cytoskeleton",
issn = "0886-1544",
publisher = "John Wiley and Sons",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells

AU - Tucker, John Barry

AU - Mogensen, MM

AU - Mackie, JB

AU - Doxsey, SJ

AU - 1, other

PY - 1997

Y1 - 1997

N2 - This report provides evidence for two functionally and spatially distinct centrosomal domains in certain mouse cochlear epithelial cells. The vast majority of microtubules elongate from sites associated with the apical cell surface in these cells rather than from pericentriolar material surrounding the immediate environs of their apically situated centrioles. The distribution of gamma-tubulin and pericentrin at cell apices has been examined while microtubule nucleation is progressing because these centrosomal proteins are believed to be essential for microtubule nucleation.Antibodies to both proteins bind to pericentriolar regions but no binding has been detected at the apical cell surface-associated sites where the ends of thousands of recently nucleated microtubules are concentrated. Sparse transient microtubule populations can be detected between pericentriolar regions and surface sites while microtubule assembly advances. A procedure apparently operates in which the pericentriolar region functions as a microtubule-nucleating domain and the cell surface-associated sites operate as docking domains which capture the minus ends of microtubules that migrate to them shortly after nucleation.Docking domains may include some components of the pericentriolar material that have been relocated at the cell apex. A docking element hypothesis for centrosomal control of minus end positioning and dynamics in animal cells generally is proposed.This investigation has also shown that the concentration of gamma-tubulin and pericentrin around centrioles differs spatially and quantitatively in ways that are characteristic for the four cell types studied. Some of these characteristics can be related to differences in control of microtubule number and positioning. (C) 1997 Wiley-Liss, Inc.

AB - This report provides evidence for two functionally and spatially distinct centrosomal domains in certain mouse cochlear epithelial cells. The vast majority of microtubules elongate from sites associated with the apical cell surface in these cells rather than from pericentriolar material surrounding the immediate environs of their apically situated centrioles. The distribution of gamma-tubulin and pericentrin at cell apices has been examined while microtubule nucleation is progressing because these centrosomal proteins are believed to be essential for microtubule nucleation.Antibodies to both proteins bind to pericentriolar regions but no binding has been detected at the apical cell surface-associated sites where the ends of thousands of recently nucleated microtubules are concentrated. Sparse transient microtubule populations can be detected between pericentriolar regions and surface sites while microtubule assembly advances. A procedure apparently operates in which the pericentriolar region functions as a microtubule-nucleating domain and the cell surface-associated sites operate as docking domains which capture the minus ends of microtubules that migrate to them shortly after nucleation.Docking domains may include some components of the pericentriolar material that have been relocated at the cell apex. A docking element hypothesis for centrosomal control of minus end positioning and dynamics in animal cells generally is proposed.This investigation has also shown that the concentration of gamma-tubulin and pericentrin around centrioles differs spatially and quantitatively in ways that are characteristic for the four cell types studied. Some of these characteristics can be related to differences in control of microtubule number and positioning. (C) 1997 Wiley-Liss, Inc.

KW - centrosomal organization

KW - microtubule-nucleation

KW - mouse cochlea

KW - ORGANIZING CENTERS

KW - MAMMALIAN-CELLS

KW - SERTOLI CELLS

KW - ORGANIZATION

KW - DROSOPHILA

KW - DYNAMICS

KW - POLARITY

KW - MITOSIS

KW - REORGANIZATION

KW - RECONSTITUTION

UR - http://www.interscience.wiley.com

M3 - Article

VL - 36

SP - 276

EP - 290

JO - Cell Motility and the Cytoskeleton

JF - Cell Motility and the Cytoskeleton

SN - 0886-1544

ER -

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