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Research at St Andrews

Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation

Research output: Other contribution

Author(s)

J Bruch, H Zitzelsberger, J Smida, L Hieber, Clare Peddie, Peter Edward Bryant, Andrew Clive Riches, J Fung, H-UG Weier, M Bauchinger

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Abstract

In vitro model cell systems are important tools for studying mechanisms of radiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma -irradiation and subsequent tumor formation in at athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as cs ell as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation-induced malignant transformation in human thyroid cells. (C) 2001 Wiley-Liss, Inc.

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Original languageEnglish
Volume96
Publication statusPublished - 20 Jun 2001

    Research areas

  • in vitro model cell system, radiation-induced tumorigenesis, chromosomal aberration, comparative genomic hybridization, spectral karyotyping, COMPARATIVE GENOMIC HYBRIDIZATION, RADIATION-INDUCED TRANSFORMATION, COPY NUMBER CHANGES, EPITHELIAL-CELLS, CELLULAR SENESCENCE, NEOPLASTIC TRANSFORMATION, INDEFINITE DIVISION, IONIZING-RADIATION, ALPHA-PARTICLES, SOLID TUMORS

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ID: 172636

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