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Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation

Research output: Other contribution

Standard

Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation. / Bruch, J; Zitzelsberger, H; Smida, J; Hieber, L; Peddie, Clare; Bryant, Peter Edward; Riches, Andrew Clive; Fung, J; Weier, H-UG; Bauchinger, M.

2001.

Research output: Other contribution

Harvard

Bruch, J, Zitzelsberger, H, Smida, J, Hieber, L, Peddie, C, Bryant, PE, Riches, AC, Fung, J, Weier, H-UG & Bauchinger, M 2001, Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation..

APA

Bruch, J., Zitzelsberger, H., Smida, J., Hieber, L., Peddie, C., Bryant, P. E., Riches, A. C., Fung, J., Weier, H-UG., & Bauchinger, M. (2001, Jun 20). Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation.

Vancouver

Bruch J, Zitzelsberger H, Smida J, Hieber L, Peddie C, Bryant PE et al. Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation. 2001.

Author

Bruch, J ; Zitzelsberger, H ; Smida, J ; Hieber, L ; Peddie, Clare ; Bryant, Peter Edward ; Riches, Andrew Clive ; Fung, J ; Weier, H-UG ; Bauchinger, M. / Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation. 2001.

Bibtex - Download

@misc{6beee54b5c3e4dbca1bacb6c228a80a0,
title = "Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation",
abstract = "In vitro model cell systems are important tools for studying mechanisms of radiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma -irradiation and subsequent tumor formation in at athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as cs ell as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation-induced malignant transformation in human thyroid cells. (C) 2001 Wiley-Liss, Inc.",
keywords = "in vitro model cell system, radiation-induced tumorigenesis, chromosomal aberration, comparative genomic hybridization, spectral karyotyping, COMPARATIVE GENOMIC HYBRIDIZATION, RADIATION-INDUCED TRANSFORMATION, COPY NUMBER CHANGES, EPITHELIAL-CELLS, CELLULAR SENESCENCE, NEOPLASTIC TRANSFORMATION, INDEFINITE DIVISION, IONIZING-RADIATION, ALPHA-PARTICLES, SOLID TUMORS",
author = "J Bruch and H Zitzelsberger and J Smida and L Hieber and Clare Peddie and Bryant, {Peter Edward} and Riches, {Andrew Clive} and J Fung and H-UG Weier and M Bauchinger",
note = "Int J Cancer (Radiat Oncol Inves )",
year = "2001",
month = jun,
day = "20",
language = "English",
volume = "96",
type = "Other",

}

RIS (suitable for import to EndNote) - Download

TY - GEN

T1 - Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation

AU - Bruch, J

AU - Zitzelsberger, H

AU - Smida, J

AU - Hieber, L

AU - Peddie, Clare

AU - Bryant, Peter Edward

AU - Riches, Andrew Clive

AU - Fung, J

AU - Weier, H-UG

AU - Bauchinger, M

N1 - Int J Cancer (Radiat Oncol Inves )

PY - 2001/6/20

Y1 - 2001/6/20

N2 - In vitro model cell systems are important tools for studying mechanisms of radiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma -irradiation and subsequent tumor formation in at athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as cs ell as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation-induced malignant transformation in human thyroid cells. (C) 2001 Wiley-Liss, Inc.

AB - In vitro model cell systems are important tools for studying mechanisms of radiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma -irradiation and subsequent tumor formation in at athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as cs ell as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation-induced malignant transformation in human thyroid cells. (C) 2001 Wiley-Liss, Inc.

KW - in vitro model cell system

KW - radiation-induced tumorigenesis

KW - chromosomal aberration

KW - comparative genomic hybridization

KW - spectral karyotyping

KW - COMPARATIVE GENOMIC HYBRIDIZATION

KW - RADIATION-INDUCED TRANSFORMATION

KW - COPY NUMBER CHANGES

KW - EPITHELIAL-CELLS

KW - CELLULAR SENESCENCE

KW - NEOPLASTIC TRANSFORMATION

KW - INDEFINITE DIVISION

KW - IONIZING-RADIATION

KW - ALPHA-PARTICLES

KW - SOLID TUMORS

M3 - Other contribution

VL - 96

ER -

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ID: 172636

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