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Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria

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Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria. / Hauton, Christopher; Brockton, V; Smith, Valerie Jane.

In: Molecular Immunology, Vol. 43, No. 9, 03.2006, p. 1490-1496.

Research output: Contribution to journalArticle

Harvard

Hauton, C, Brockton, V & Smith, VJ 2006, 'Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria' Molecular Immunology, vol. 43, no. 9, pp. 1490-1496. https://doi.org/10.1016/j.molimm.2005.07.029

APA

Hauton, C., Brockton, V., & Smith, V. J. (2006). Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria. Molecular Immunology, 43(9), 1490-1496. https://doi.org/10.1016/j.molimm.2005.07.029

Vancouver

Hauton C, Brockton V, Smith VJ. Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria. Molecular Immunology. 2006 Mar;43(9):1490-1496. https://doi.org/10.1016/j.molimm.2005.07.029

Author

Hauton, Christopher ; Brockton, V ; Smith, Valerie Jane. / Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria. In: Molecular Immunology. 2006 ; Vol. 43, No. 9. pp. 1490-1496.

Bibtex - Download

@article{23d695c8520f4edf89fd8fb93c66709c,
title = "Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria",
abstract = "Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The inferred amino acid sequence of this peptide was found to be similar to crustin-like peptides isolated for several species of shrimp as well as the shore crab, Carcinus maenas. The sequence also contains a single-whey-acidic protein (WAP) domain, similar to novel antibacterial single-whey-acidic domain (SWD) peptides that have been recently described in the tiger shrimp, Penaeus monodon, and the Pacific white shrimp, Litopenaeus vannamei. Real-time PCR was used to analyse the expression of the gene coding for this peptide. The gene is up regulated after inoculation with the Gram-positive lobster pathogen Aerococcus viridans var. homari but down regulated after inoculation with the Gram-negative bacteria Listonella anguillarum. Phylogenetic analysis of this new peptide shows that it is most related to other antimicrobial crustin peptides and that the crustins are only distantly related to the antibacterial SWD peptides recently described. (c) 2005 Elsevier Ltd. All rights reserved.",
keywords = "Homarus gammarus, antibacterial peptide, crustin, WAP domain, real-time PCR, ANTIMICROBIAL PEPTIDES, LITOPENAEUS-VANNAMEI, MOLECULAR-CLONING, RECOMBINANT EXPRESSION, GRANULAR HEMOCYTES, CARCINUS-MAENAS, PENAEID SHRIMP, SHORE CRAB, PROTEIN, INHIBITOR",
author = "Christopher Hauton and V Brockton and Smith, {Valerie Jane}",
year = "2006",
month = "3",
doi = "10.1016/j.molimm.2005.07.029",
language = "English",
volume = "43",
pages = "1490--1496",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier",
number = "9",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cloning of a crustin-like, single whey-acidic-domain antibacterial peptide from haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria

AU - Hauton, Christopher

AU - Brockton, V

AU - Smith, Valerie Jane

PY - 2006/3

Y1 - 2006/3

N2 - Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The inferred amino acid sequence of this peptide was found to be similar to crustin-like peptides isolated for several species of shrimp as well as the shore crab, Carcinus maenas. The sequence also contains a single-whey-acidic protein (WAP) domain, similar to novel antibacterial single-whey-acidic domain (SWD) peptides that have been recently described in the tiger shrimp, Penaeus monodon, and the Pacific white shrimp, Litopenaeus vannamei. Real-time PCR was used to analyse the expression of the gene coding for this peptide. The gene is up regulated after inoculation with the Gram-positive lobster pathogen Aerococcus viridans var. homari but down regulated after inoculation with the Gram-negative bacteria Listonella anguillarum. Phylogenetic analysis of this new peptide shows that it is most related to other antimicrobial crustin peptides and that the crustins are only distantly related to the antibacterial SWD peptides recently described. (c) 2005 Elsevier Ltd. All rights reserved.

AB - Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The inferred amino acid sequence of this peptide was found to be similar to crustin-like peptides isolated for several species of shrimp as well as the shore crab, Carcinus maenas. The sequence also contains a single-whey-acidic protein (WAP) domain, similar to novel antibacterial single-whey-acidic domain (SWD) peptides that have been recently described in the tiger shrimp, Penaeus monodon, and the Pacific white shrimp, Litopenaeus vannamei. Real-time PCR was used to analyse the expression of the gene coding for this peptide. The gene is up regulated after inoculation with the Gram-positive lobster pathogen Aerococcus viridans var. homari but down regulated after inoculation with the Gram-negative bacteria Listonella anguillarum. Phylogenetic analysis of this new peptide shows that it is most related to other antimicrobial crustin peptides and that the crustins are only distantly related to the antibacterial SWD peptides recently described. (c) 2005 Elsevier Ltd. All rights reserved.

KW - Homarus gammarus

KW - antibacterial peptide

KW - crustin

KW - WAP domain

KW - real-time PCR

KW - ANTIMICROBIAL PEPTIDES

KW - LITOPENAEUS-VANNAMEI

KW - MOLECULAR-CLONING

KW - RECOMBINANT EXPRESSION

KW - GRANULAR HEMOCYTES

KW - CARCINUS-MAENAS

KW - PENAEID SHRIMP

KW - SHORE CRAB

KW - PROTEIN

KW - INHIBITOR

UR - http://www.scopus.com/inward/record.url?scp=33644829821&partnerID=8YFLogxK

U2 - 10.1016/j.molimm.2005.07.029

DO - 10.1016/j.molimm.2005.07.029

M3 - Article

VL - 43

SP - 1490

EP - 1496

JO - Molecular Immunology

T2 - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 9

ER -

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ID: 384635