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Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity

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Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity. / Hammond, JA; Nakao, M; Smith, Valerie Jane.

In: Molecular Immunology, Vol. 42, No. 6, 04.2005, p. 683-694.

Research output: Contribution to journalArticle

Harvard

Hammond, JA, Nakao, M & Smith, VJ 2005, 'Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity' Molecular Immunology, vol. 42, no. 6, pp. 683-694. https://doi.org/10.1016/j.molimm.2004.09.017

APA

Hammond, JA., Nakao, M., & Smith, V. J. (2005). Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity. Molecular Immunology, 42(6), 683-694. https://doi.org/10.1016/j.molimm.2004.09.017

Vancouver

Hammond JA, Nakao M, Smith VJ. Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity. Molecular Immunology. 2005 Apr;42(6):683-694. https://doi.org/10.1016/j.molimm.2004.09.017

Author

Hammond, JA ; Nakao, M ; Smith, Valerie Jane. / Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity. In: Molecular Immunology. 2005 ; Vol. 42, No. 6. pp. 683-694.

Bibtex - Download

@article{f250d81c064d4897b24978213afaa1c3,
title = "Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity",
abstract = "Molecular approaches were used to study thiolester-containing genes in the ascidian, Ciona intestinalis. RT-PCR, RACE and genome mining revealed that this animal expresses not only conventional alpha-2-macroglobulin (alpha 2m) and two forms of C3 but also a gene encoding a glycosylphosphatidylinositol (GPI)-anchored alpha 2m. Previously, GPI-anchored alpha 2ms have been reported only for humans and mice. We propose that GPI-anchored a2ms constitute a third subgroup of the a2m superfamily and may represent an important evolutionary stage in the phylogeny of the thiolester containing proteins. Its occurrence in an ascidian shows its origin pre-dates the evolution of the vertebrates. In C. intestinalis this GPI-anchored a2m, designated Ciona alpha 2m-GPI, is expressed in the hepatopancreas, circulating coelomic blood cells and the ut of adults. It is also expressed in 3-5 days old larvae. Its tissue distribution coupled with its sequence characteristics and unusual domain structure indicate that the encoded protein probably assists in host defence by entrapping and inhibiting proteases from micro-organisms. (c) 2004 Elsevier Ltd. All rights reserved.",
keywords = "alpha-2-macroglobulin, glycosylphosphatidylinositol (GPI)-anchorage, thiolester-containing proteins, Ascidian, Ciona, innate immunity, ALPHA-MACROGLOBULIN RECEPTOR, HUMAN T-CELLS, COMPLEMENT-SYSTEM, CONTAINING PROTEINS, ALPHA(2)-MACROGLOBULIN, EVOLUTION, IDENTIFICATION, THIOESTER, PLATELETS, BINDING",
author = "JA Hammond and M Nakao and Smith, {Valerie Jane}",
year = "2005",
month = "4",
doi = "10.1016/j.molimm.2004.09.017",
language = "English",
volume = "42",
pages = "683--694",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cloning of a glycosylphosphatidylinositol-anchored alpha-2-macroglobulin cDNA from the ascidian, Ciona intestinalis, and its possible role in immunity

AU - Hammond, JA

AU - Nakao, M

AU - Smith, Valerie Jane

PY - 2005/4

Y1 - 2005/4

N2 - Molecular approaches were used to study thiolester-containing genes in the ascidian, Ciona intestinalis. RT-PCR, RACE and genome mining revealed that this animal expresses not only conventional alpha-2-macroglobulin (alpha 2m) and two forms of C3 but also a gene encoding a glycosylphosphatidylinositol (GPI)-anchored alpha 2m. Previously, GPI-anchored alpha 2ms have been reported only for humans and mice. We propose that GPI-anchored a2ms constitute a third subgroup of the a2m superfamily and may represent an important evolutionary stage in the phylogeny of the thiolester containing proteins. Its occurrence in an ascidian shows its origin pre-dates the evolution of the vertebrates. In C. intestinalis this GPI-anchored a2m, designated Ciona alpha 2m-GPI, is expressed in the hepatopancreas, circulating coelomic blood cells and the ut of adults. It is also expressed in 3-5 days old larvae. Its tissue distribution coupled with its sequence characteristics and unusual domain structure indicate that the encoded protein probably assists in host defence by entrapping and inhibiting proteases from micro-organisms. (c) 2004 Elsevier Ltd. All rights reserved.

AB - Molecular approaches were used to study thiolester-containing genes in the ascidian, Ciona intestinalis. RT-PCR, RACE and genome mining revealed that this animal expresses not only conventional alpha-2-macroglobulin (alpha 2m) and two forms of C3 but also a gene encoding a glycosylphosphatidylinositol (GPI)-anchored alpha 2m. Previously, GPI-anchored alpha 2ms have been reported only for humans and mice. We propose that GPI-anchored a2ms constitute a third subgroup of the a2m superfamily and may represent an important evolutionary stage in the phylogeny of the thiolester containing proteins. Its occurrence in an ascidian shows its origin pre-dates the evolution of the vertebrates. In C. intestinalis this GPI-anchored a2m, designated Ciona alpha 2m-GPI, is expressed in the hepatopancreas, circulating coelomic blood cells and the ut of adults. It is also expressed in 3-5 days old larvae. Its tissue distribution coupled with its sequence characteristics and unusual domain structure indicate that the encoded protein probably assists in host defence by entrapping and inhibiting proteases from micro-organisms. (c) 2004 Elsevier Ltd. All rights reserved.

KW - alpha-2-macroglobulin

KW - glycosylphosphatidylinositol (GPI)-anchorage

KW - thiolester-containing proteins

KW - Ascidian

KW - Ciona

KW - innate immunity

KW - ALPHA-MACROGLOBULIN RECEPTOR

KW - HUMAN T-CELLS

KW - COMPLEMENT-SYSTEM

KW - CONTAINING PROTEINS

KW - ALPHA(2)-MACROGLOBULIN

KW - EVOLUTION

KW - IDENTIFICATION

KW - THIOESTER

KW - PLATELETS

KW - BINDING

U2 - 10.1016/j.molimm.2004.09.017

DO - 10.1016/j.molimm.2004.09.017

M3 - Article

VL - 42

SP - 683

EP - 694

JO - Molecular Immunology

T2 - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 6

ER -

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ID: 300028