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Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications

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Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications. / Wikraiphat, Chanthiwa; Saiprom, Natnaree; Tandhavanant, Sarunporn; Heiss, Christian; Azadi, Parastoo; Wongsuvan, Gumphol; Tuanyok, Apichai; Holden, Matthew; Burtnick, Mary N; Brett, Paul J; Peacock, Sharon J; Chantratita, Narisara.

In: Infection and Immunity, Vol. 83, No. 5, 05.2015, p. 2127-2138.

Research output: Contribution to journalArticle

Harvard

Wikraiphat, C, Saiprom, N, Tandhavanant, S, Heiss, C, Azadi, P, Wongsuvan, G, Tuanyok, A, Holden, M, Burtnick, MN, Brett, PJ, Peacock, SJ & Chantratita, N 2015, 'Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications', Infection and Immunity, vol. 83, no. 5, pp. 2127-2138. https://doi.org/10.1128/IAI.02785-14

APA

Wikraiphat, C., Saiprom, N., Tandhavanant, S., Heiss, C., Azadi, P., Wongsuvan, G., ... Chantratita, N. (2015). Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications. Infection and Immunity, 83(5), 2127-2138. https://doi.org/10.1128/IAI.02785-14

Vancouver

Wikraiphat C, Saiprom N, Tandhavanant S, Heiss C, Azadi P, Wongsuvan G et al. Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications. Infection and Immunity. 2015 May;83(5):2127-2138. https://doi.org/10.1128/IAI.02785-14

Author

Wikraiphat, Chanthiwa ; Saiprom, Natnaree ; Tandhavanant, Sarunporn ; Heiss, Christian ; Azadi, Parastoo ; Wongsuvan, Gumphol ; Tuanyok, Apichai ; Holden, Matthew ; Burtnick, Mary N ; Brett, Paul J ; Peacock, Sharon J ; Chantratita, Narisara. / Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications. In: Infection and Immunity. 2015 ; Vol. 83, No. 5. pp. 2127-2138.

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@article{42aa20dbb830428ca09ffedd4f747655,
title = "Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications",
abstract = "Burkholderia pseudomallei is a CDC Tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS types. The predominant O-polysaccharide (OPS) is an unbranched heteropolymer with repeating d-glucose and 6-deoxy-l-talose residues in which the 6-deoxy-l-talose residues are variably substituted with O-acetyl and O-methyl modifications. We observed that primary clinical B. pseudomallei isolates with mucoid and non-mucoid colony morphologies from the same sample expressed different antigenic types distinguishable using a LPS-specific monoclonal antibody (Mab). Mab reactive (non-mucoid) and non-reactive (mucoid) strains from the same patient exhibited identical LPS banding patterns by silver staining and indistinguishable genotypes. We hypothesized that LPS antigenic variation reflected modification of the OPS moieties. Mutagenesis of three genes involved in LPS synthesis was performed in B. pseudomallei K96243. Loss of Mab reactivity was observed in both wbiA (encoding a 2-O-acetyltransferase) and wbiD (putative methyl transferase) mutants. The structural characteristics of the OPS moieties from isogenic non-mucoid strain 4095a and mucoid strain 4095c were further investigated. Utilizing NMR spectroscopy, we found that B. pseudomallei 4095a and 4095c OPS antigens exhibited substitution patterns that differed from the prototypic OPS structure. Specifically, 4095a lacked 4-O-acetylation while 4095c lacked both 4-O-acetylation and 2-O-methylation. Our studies indicate that B. pseudomallei OPS undergoes antigenic variation and suggest that the 9D5 Mab recognizes a conformational epitope that is influenced by both O-acetyl and O-methyl substitution patterns.",
keywords = "Melioidosis, Burkholderia pseudomallei, Lipopolysaccharide, O-polysaccharide",
author = "Chanthiwa Wikraiphat and Natnaree Saiprom and Sarunporn Tandhavanant and Christian Heiss and Parastoo Azadi and Gumphol Wongsuvan and Apichai Tuanyok and Matthew Holden and Burtnick, {Mary N} and Brett, {Paul J} and Peacock, {Sharon J} and Narisara Chantratita",
note = "This work was supported by the Thailand Research Fund (grantTRG5580004), the Wellcome Trust, United Kingdom, and the Department of Energy-funded Center for Plant and Microbial Complex Carbohydrates (grant DE-FG09-93ER-20097). N.C. was supported by the Wellcome Trust (grant 087769/Z/08/Z).",
year = "2015",
month = "5",
doi = "10.1128/IAI.02785-14",
language = "English",
volume = "83",
pages = "2127--2138",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "AMER SOC MICROBIOLOGY",
number = "5",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications

AU - Wikraiphat, Chanthiwa

AU - Saiprom, Natnaree

AU - Tandhavanant, Sarunporn

AU - Heiss, Christian

AU - Azadi, Parastoo

AU - Wongsuvan, Gumphol

AU - Tuanyok, Apichai

AU - Holden, Matthew

AU - Burtnick, Mary N

AU - Brett, Paul J

AU - Peacock, Sharon J

AU - Chantratita, Narisara

N1 - This work was supported by the Thailand Research Fund (grantTRG5580004), the Wellcome Trust, United Kingdom, and the Department of Energy-funded Center for Plant and Microbial Complex Carbohydrates (grant DE-FG09-93ER-20097). N.C. was supported by the Wellcome Trust (grant 087769/Z/08/Z).

PY - 2015/5

Y1 - 2015/5

N2 - Burkholderia pseudomallei is a CDC Tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS types. The predominant O-polysaccharide (OPS) is an unbranched heteropolymer with repeating d-glucose and 6-deoxy-l-talose residues in which the 6-deoxy-l-talose residues are variably substituted with O-acetyl and O-methyl modifications. We observed that primary clinical B. pseudomallei isolates with mucoid and non-mucoid colony morphologies from the same sample expressed different antigenic types distinguishable using a LPS-specific monoclonal antibody (Mab). Mab reactive (non-mucoid) and non-reactive (mucoid) strains from the same patient exhibited identical LPS banding patterns by silver staining and indistinguishable genotypes. We hypothesized that LPS antigenic variation reflected modification of the OPS moieties. Mutagenesis of three genes involved in LPS synthesis was performed in B. pseudomallei K96243. Loss of Mab reactivity was observed in both wbiA (encoding a 2-O-acetyltransferase) and wbiD (putative methyl transferase) mutants. The structural characteristics of the OPS moieties from isogenic non-mucoid strain 4095a and mucoid strain 4095c were further investigated. Utilizing NMR spectroscopy, we found that B. pseudomallei 4095a and 4095c OPS antigens exhibited substitution patterns that differed from the prototypic OPS structure. Specifically, 4095a lacked 4-O-acetylation while 4095c lacked both 4-O-acetylation and 2-O-methylation. Our studies indicate that B. pseudomallei OPS undergoes antigenic variation and suggest that the 9D5 Mab recognizes a conformational epitope that is influenced by both O-acetyl and O-methyl substitution patterns.

AB - Burkholderia pseudomallei is a CDC Tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS types. The predominant O-polysaccharide (OPS) is an unbranched heteropolymer with repeating d-glucose and 6-deoxy-l-talose residues in which the 6-deoxy-l-talose residues are variably substituted with O-acetyl and O-methyl modifications. We observed that primary clinical B. pseudomallei isolates with mucoid and non-mucoid colony morphologies from the same sample expressed different antigenic types distinguishable using a LPS-specific monoclonal antibody (Mab). Mab reactive (non-mucoid) and non-reactive (mucoid) strains from the same patient exhibited identical LPS banding patterns by silver staining and indistinguishable genotypes. We hypothesized that LPS antigenic variation reflected modification of the OPS moieties. Mutagenesis of three genes involved in LPS synthesis was performed in B. pseudomallei K96243. Loss of Mab reactivity was observed in both wbiA (encoding a 2-O-acetyltransferase) and wbiD (putative methyl transferase) mutants. The structural characteristics of the OPS moieties from isogenic non-mucoid strain 4095a and mucoid strain 4095c were further investigated. Utilizing NMR spectroscopy, we found that B. pseudomallei 4095a and 4095c OPS antigens exhibited substitution patterns that differed from the prototypic OPS structure. Specifically, 4095a lacked 4-O-acetylation while 4095c lacked both 4-O-acetylation and 2-O-methylation. Our studies indicate that B. pseudomallei OPS undergoes antigenic variation and suggest that the 9D5 Mab recognizes a conformational epitope that is influenced by both O-acetyl and O-methyl substitution patterns.

KW - Melioidosis

KW - Burkholderia pseudomallei

KW - Lipopolysaccharide

KW - O-polysaccharide

U2 - 10.1128/IAI.02785-14

DO - 10.1128/IAI.02785-14

M3 - Article

VL - 83

SP - 2127

EP - 2138

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -

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