Skip to content

Research at St Andrews

Combination treatment with anti-Nogo-A and chondroitinase ABC is more effective than single treatments at enhancing functional recovery after spinal cord injury

Research output: Contribution to journalArticlepeer-review

DOI

Author(s)

Rongrong Zhao, Melissa Renee Andrews, Difei Wang, Philippa Warren, Miriam Gullo, Lisa Schnell, Martin E Schwab, James W Fawcett

School/Research organisations

Abstract

Anti-Nogo-A antibody and chondroitinase ABC (ChABC) enzyme are two promising treatments that promote functional recovery after spinal cord injury (SCI). Treatment with them has encouraged axon regeneration, sprouting and functional recovery in a variety of spinal cord and central nervous system injury models. The two compounds work, in part, through different mechanisms, so it is possible that their effects will be additive. In this study, we used a rat cervical partial SCI model to explore the effectiveness of a combination of anti-Nogo-A, ChABC, and rehabilitation. We found that spontaneous recovery of forelimb functions reflects the extent of the lesion on the ipsilateral side. We applied a combination treatment with acutely applied anti-Nogo-A antibody followed by delayed ChABC treatment starting at 3 weeks after injury, and rehabilitation starting at 4 weeks, to accommodate the requirement that anti-Nogo-A be applied acutely, and that rehabilitation be given after the cessation of anti-Nogo-A treatment. We found that single treatment with either anti-Nogo-A or ChABC, combined with rehabilitation, produced functional recovery of similar magnitude. The combination treatment, however, was more effective. Both single treatments produced increases in sprouting and axon regeneration, but the combination treatment produced greater increases. Anti-Nogo-A stimulated growth of a greater number of axons with a diameter of > 3 μm, whereas ChABC treatment stimulated increased growth of finer axons with varicosities. These results point to different functions of Nogo-A and chondroitin sulfate proteoglycans in axonal regeneration. The combination of anti-Nogo-A, ChABC and rehabilitation shows promise for enhancing functional recovery after SCI.
Close

Details

Original languageEnglish
Pages (from-to)2946-2961
JournalEuropean Journal of Neuroscience
Volume38
Issue number6
Early online date24 Jun 2013
DOIs
Publication statusPublished - Sep 2013

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by journal

  1. Mobile EEG reveals functionally dissociable dynamic processes supporting real-world ambulatory obstacle avoidance: evidence for early proactive control

    Mustile, M., Kourtis, D., Ladouce, S., Learmonth, G., Donaldson, D. I., Edwards, M. G. & Ietswaart, M., 19 Jan 2021, (Accepted/In press) In: European Journal of Neuroscience. In press

    Research output: Contribution to journalArticlepeer-review

  2. A simple rule to describe interactions between visual categories

    Poncet, M., Fabre-Thorpe, M. & Chakravarthi, R., 31 Dec 2020, In: European Journal of Neuroscience. 52, 12, p. 4639-4666 28 p.

    Research output: Contribution to journalArticlepeer-review

  3. The neuronal migration hypothesis of dyslexia: a critical evaluation 30 years on

    Guidi, L. G., Velayos-Baeza, A., Martinez-Garay, I., Monaca, A. P., Paracchini, S., Bishop, D. V. M. & Molnár, Z., Nov 2018, In: European Journal of Neuroscience. 48, 10, p. 3212-3233 22 p.

    Research output: Contribution to journalReview articlepeer-review

  4. Visual system integrity and cognition in early Huntington's disease

    Wolf, R. C., Sambataro, F., Vasic, N., Baldas, E-M., Ratheiser, I., Landwehrmeyer, G. B., Depping, M. S., Thomann, P. A., Sprengelmeyer, R. H., Süssmuth, S. S. & Orth, M., Jul 2014, In: European Journal of Neuroscience. 40, 2, p. 2417–2426 10 p.

    Research output: Contribution to journalArticlepeer-review

ID: 56380635

Top