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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing. / Bossé, Janine T; Li, Yanwen; Fernandez Crespo, Roberto; Lacouture, Sonia; Gottschalk, Marcelo; Sárközi, Rita; Fodor, László; Casas Amoribieta, Maria; Angen, Øystein; Nedbalcova, Katerina; Holden, Matthew T. G.; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; Rycroft, Andrew N; Langford, Paul R; BRaDP1T Consortium.

In: Veterinary Microbiology, Vol. 220, 07.2018, p. 83-89.

Research output: Contribution to journalArticle

Harvard

Bossé, JT, Li, Y, Fernandez Crespo, R, Lacouture, S, Gottschalk, M, Sárközi, R, Fodor, L, Casas Amoribieta, M, Angen, Ø, Nedbalcova, K, Holden, MTG, Maskell, DJ, Tucker, AW, Wren, BW, Rycroft, AN, Langford, PR & BRaDP1T Consortium 2018, 'Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing', Veterinary Microbiology, vol. 220, pp. 83-89. https://doi.org/10.1016/j.vetmic.2018.05.011

APA

Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., ... BRaDP1T Consortium (2018). Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing. Veterinary Microbiology, 220, 83-89. https://doi.org/10.1016/j.vetmic.2018.05.011

Vancouver

Bossé JT, Li Y, Fernandez Crespo R, Lacouture S, Gottschalk M, Sárközi R et al. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing. Veterinary Microbiology. 2018 Jul;220:83-89. https://doi.org/10.1016/j.vetmic.2018.05.011

Author

Bossé, Janine T ; Li, Yanwen ; Fernandez Crespo, Roberto ; Lacouture, Sonia ; Gottschalk, Marcelo ; Sárközi, Rita ; Fodor, László ; Casas Amoribieta, Maria ; Angen, Øystein ; Nedbalcova, Katerina ; Holden, Matthew T. G. ; Maskell, Duncan J ; Tucker, Alexander W ; Wren, Brendan W ; Rycroft, Andrew N ; Langford, Paul R ; BRaDP1T Consortium. / Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing. In: Veterinary Microbiology. 2018 ; Vol. 220. pp. 83-89.

Bibtex - Download

@article{f32264b24a4344e88fd0a7deba86a6dd,
title = "Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing",
abstract = "Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.",
keywords = "Diagnostic, mPCR, Capsule typing, A. pleuropneumoniae, Serovars",
author = "Boss{\'e}, {Janine T} and Yanwen Li and {Fernandez Crespo}, Roberto and Sonia Lacouture and Marcelo Gottschalk and Rita S{\'a}rk{\"o}zi and L{\'a}szl{\'o} Fodor and {Casas Amoribieta}, Maria and {\O}ystein Angen and Katerina Nedbalcova and Holden, {Matthew T. G.} and Maskell, {Duncan J} and Tucker, {Alexander W} and Wren, {Brendan W} and Rycroft, {Andrew N} and Langford, {Paul R} and {BRaDP1T Consortium}",
note = "This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1 and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MTGH was supported by the Wellcome Trust (grant number 098051). RS and LF were supported by the Hungarian Scientific Research Fund (OTKA 112826). Genome sequencing provided by MicrobesNG (www.microbesng.uk) was supported by the BBSRC (grant number BB/L024209/1).",
year = "2018",
month = "7",
doi = "10.1016/j.vetmic.2018.05.011",
language = "English",
volume = "220",
pages = "83--89",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

AU - Bossé, Janine T

AU - Li, Yanwen

AU - Fernandez Crespo, Roberto

AU - Lacouture, Sonia

AU - Gottschalk, Marcelo

AU - Sárközi, Rita

AU - Fodor, László

AU - Casas Amoribieta, Maria

AU - Angen, Øystein

AU - Nedbalcova, Katerina

AU - Holden, Matthew T. G.

AU - Maskell, Duncan J

AU - Tucker, Alexander W

AU - Wren, Brendan W

AU - Rycroft, Andrew N

AU - Langford, Paul R

AU - BRaDP1T Consortium

N1 - This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1 and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MTGH was supported by the Wellcome Trust (grant number 098051). RS and LF were supported by the Hungarian Scientific Research Fund (OTKA 112826). Genome sequencing provided by MicrobesNG (www.microbesng.uk) was supported by the BBSRC (grant number BB/L024209/1).

PY - 2018/7

Y1 - 2018/7

N2 - Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

AB - Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

KW - Diagnostic

KW - mPCR

KW - Capsule typing

KW - A. pleuropneumoniae

KW - Serovars

U2 - 10.1016/j.vetmic.2018.05.011

DO - 10.1016/j.vetmic.2018.05.011

M3 - Article

VL - 220

SP - 83

EP - 89

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -

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