Skip to content

Research at St Andrews

Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)

Research output: Contribution to journalArticle

Author(s)

Daniel Garcia de la Serrana, Edson A. Mareco, Vera L. A. Vieira, Deborah M. Power, Ian A. Johnston

School/Research organisations

Abstract

Skeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. In the present study on the gilthead sea bream (Sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. To investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose of the branched chain amino acid leucine and compared the results with saline-injected controls. To increase the amount of available sequence information for gene expression analysis a de novo transcriptome was assembled using publicly available Next Generation Sequencing libraries from embryo, fast skeletal muscle, bone and cartilage. The resulting 5 million reads were assembled into 125,646 isotigs representing around 16,000 unique genes, including most components of the Pi3k/Akt/mTor signalling pathway. Principal Components Analysis was able to distinguish the transcriptional responses between leucine and saline injected controls in skeletal muscle, but not in bone. General Linear Modelling revealed significant temporal changes in gene expression following leucine injection including the tissue-specific markers sparc, bglap (bone), mlc2 and myod2 (muscle) and gene transcripts associated with Pi3k/Akt/mTor signalling, p70sk6, akt2, ampka and mtor. Skeletal muscle showed more pronounced and rapid changes in transcript abundance than bone to the same pro-growth signal. The observed differences in transcriptional response are consistent with the idea that fast growth results in a miss-match between muscle and bone development and may contribute to a higher incidence of skeletal deformities.
Close

Details

Original languageEnglish
Pages (from-to)50-57
Number of pages7
JournalComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
Volume199
Early online date26 Feb 2016
DOIs
Publication statusPublished - Sep 2016

    Research areas

  • NGS, Muscle, Bone, Expression, Leucine, mTOR, Fast growth, Aquaculture, Skeletal deformities

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Influence of feed ration size on somatic and muscle growth in landlocked dwarf and farmed Atlantic salmon Salmo salar

    Andersen, Ø., Vieira, V., Dessen, J-E. & Johnston, I. A., Apr 2019, In : Journal of Fish Biology. 94, 4, p. 614-620 7 p.

    Research output: Contribution to journalArticle

  2. Genomic tools and selective breeding in molluscs

    Hollenbeck, C. M. & Johnston, I. A., 18 Jul 2018, In : Frontiers in Genetics. 9, 15 p., 253.

    Research output: Contribution to journalReview article

  3. A collaborative European approach to accelerating translational marine science

    Brennecke, P., Ferrante, M., Johnston, I. A. & Smith, D., 5 Jul 2018, In : Journal of Marine Science and Engineering. 6, 3, 12 p., 81.

    Research output: Contribution to journalArticle

  4. A workflow used to design low density SNP panels for parentage assignment and traceability in aquaculture species and its validation in Atlantic salmon

    Holman, L. E., Garcia de la Serrana, D., Onoufriou, A., Hillestad, B. & Johnston, I. A., 1 Jul 2017, In : Aquaculture. 476, p. 59-64 6 p.

    Research output: Contribution to journalArticle

Related by journal

ID: 241247502