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Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)

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Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata). / Garcia de la Serrana, Daniel; Mareco, Edson A.; Vieira, Vera L. A.; Power, Deborah M.; Johnston, Ian A.

In: Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, Vol. 199, 09.2016, p. 50-57.

Research output: Contribution to journalArticle

Harvard

Garcia de la Serrana, D, Mareco, EA, Vieira, VLA, Power, DM & Johnston, IA 2016, 'Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)' Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, vol. 199, pp. 50-57. https://doi.org/10.1016/j.cbpb.2016.02.003

APA

Garcia de la Serrana, D., Mareco, E. A., Vieira, V. L. A., Power, D. M., & Johnston, I. A. (2016). Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata). Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 199, 50-57. https://doi.org/10.1016/j.cbpb.2016.02.003

Vancouver

Garcia de la Serrana D, Mareco EA, Vieira VLA, Power DM, Johnston IA. Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata). Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology. 2016 Sep;199:50-57. https://doi.org/10.1016/j.cbpb.2016.02.003

Author

Garcia de la Serrana, Daniel ; Mareco, Edson A. ; Vieira, Vera L. A. ; Power, Deborah M. ; Johnston, Ian A. / Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata). In: Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology. 2016 ; Vol. 199. pp. 50-57.

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@article{65ec140592e240ff8d6e02e1d3c56837,
title = "Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)",
abstract = "Skeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. In the present study on the gilthead sea bream (Sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. To investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose of the branched chain amino acid leucine and compared the results with saline-injected controls. To increase the amount of available sequence information for gene expression analysis a de novo transcriptome was assembled using publicly available Next Generation Sequencing libraries from embryo, fast skeletal muscle, bone and cartilage. The resulting 5 million reads were assembled into 125,646 isotigs representing around 16,000 unique genes, including most components of the Pi3k/Akt/mTor signalling pathway. Principal Components Analysis was able to distinguish the transcriptional responses between leucine and saline injected controls in skeletal muscle, but not in bone. General Linear Modelling revealed significant temporal changes in gene expression following leucine injection including the tissue-specific markers sparc, bglap (bone), mlc2 and myod2 (muscle) and gene transcripts associated with Pi3k/Akt/mTor signalling, p70sk6, akt2, ampka and mtor. Skeletal muscle showed more pronounced and rapid changes in transcript abundance than bone to the same pro-growth signal. The observed differences in transcriptional response are consistent with the idea that fast growth results in a miss-match between muscle and bone development and may contribute to a higher incidence of skeletal deformities.",
keywords = "NGS, Muscle, Bone, Expression, Leucine, mTOR, Fast growth, Aquaculture, Skeletal deformities",
author = "{Garcia de la Serrana}, Daniel and Mareco, {Edson A.} and Vieira, {Vera L. A.} and Power, {Deborah M.} and Johnston, {Ian A.}",
note = "This research was funded by the European Community's Seventh Framework program (FP7/2007–2013) under grant agreement No 222,719- LIFECYCLE and by an EU Research Infrastructure Action under the FP7 “Capacities” Programme, ASSEMBLE (no. 227,799, CCMAR Ref 00,215/2011) and by the Marine Science & Technology pooling initiative and Scottish Funding Council grant HR09011 and contributing institutions.",
year = "2016",
month = "9",
doi = "10.1016/j.cbpb.2016.02.003",
language = "English",
volume = "199",
pages = "50--57",
journal = "Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology",
issn = "1096-4959",
publisher = "Elsevier Inc.",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (Sparus aurata)

AU - Garcia de la Serrana, Daniel

AU - Mareco, Edson A.

AU - Vieira, Vera L. A.

AU - Power, Deborah M.

AU - Johnston, Ian A.

N1 - This research was funded by the European Community's Seventh Framework program (FP7/2007–2013) under grant agreement No 222,719- LIFECYCLE and by an EU Research Infrastructure Action under the FP7 “Capacities” Programme, ASSEMBLE (no. 227,799, CCMAR Ref 00,215/2011) and by the Marine Science & Technology pooling initiative and Scottish Funding Council grant HR09011 and contributing institutions.

PY - 2016/9

Y1 - 2016/9

N2 - Skeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. In the present study on the gilthead sea bream (Sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. To investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose of the branched chain amino acid leucine and compared the results with saline-injected controls. To increase the amount of available sequence information for gene expression analysis a de novo transcriptome was assembled using publicly available Next Generation Sequencing libraries from embryo, fast skeletal muscle, bone and cartilage. The resulting 5 million reads were assembled into 125,646 isotigs representing around 16,000 unique genes, including most components of the Pi3k/Akt/mTor signalling pathway. Principal Components Analysis was able to distinguish the transcriptional responses between leucine and saline injected controls in skeletal muscle, but not in bone. General Linear Modelling revealed significant temporal changes in gene expression following leucine injection including the tissue-specific markers sparc, bglap (bone), mlc2 and myod2 (muscle) and gene transcripts associated with Pi3k/Akt/mTor signalling, p70sk6, akt2, ampka and mtor. Skeletal muscle showed more pronounced and rapid changes in transcript abundance than bone to the same pro-growth signal. The observed differences in transcriptional response are consistent with the idea that fast growth results in a miss-match between muscle and bone development and may contribute to a higher incidence of skeletal deformities.

AB - Skeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. In the present study on the gilthead sea bream (Sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. To investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose of the branched chain amino acid leucine and compared the results with saline-injected controls. To increase the amount of available sequence information for gene expression analysis a de novo transcriptome was assembled using publicly available Next Generation Sequencing libraries from embryo, fast skeletal muscle, bone and cartilage. The resulting 5 million reads were assembled into 125,646 isotigs representing around 16,000 unique genes, including most components of the Pi3k/Akt/mTor signalling pathway. Principal Components Analysis was able to distinguish the transcriptional responses between leucine and saline injected controls in skeletal muscle, but not in bone. General Linear Modelling revealed significant temporal changes in gene expression following leucine injection including the tissue-specific markers sparc, bglap (bone), mlc2 and myod2 (muscle) and gene transcripts associated with Pi3k/Akt/mTor signalling, p70sk6, akt2, ampka and mtor. Skeletal muscle showed more pronounced and rapid changes in transcript abundance than bone to the same pro-growth signal. The observed differences in transcriptional response are consistent with the idea that fast growth results in a miss-match between muscle and bone development and may contribute to a higher incidence of skeletal deformities.

KW - NGS

KW - Muscle

KW - Bone

KW - Expression

KW - Leucine

KW - mTOR

KW - Fast growth

KW - Aquaculture

KW - Skeletal deformities

U2 - 10.1016/j.cbpb.2016.02.003

DO - 10.1016/j.cbpb.2016.02.003

M3 - Article

VL - 199

SP - 50

EP - 57

JO - Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology

T2 - Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology

SN - 1096-4959

ER -

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