Skip to content

Research at St Andrews

Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

Research output: Contribution to journalArticlepeer-review

DOI

Open Access permissions

Open

Author(s)

Pau Pulido Companys, Anneliese Norris, Marcus Bischoff

School/Research organisations

Abstract

During morphogenesis, cells exhibit various behaviours, such as migration and constriction, which need to be coordinated. How this is achieved remains elusive. During morphogenesis of the Drosophila adult abdominal epidermis, larval epithelial cells (LECs) migrate directedly before constricting apically and undergoing apoptosis. Here, we study the mechanisms underlying the transition from migration to constriction. We show that LECs possess a pulsatile apical actomyosin network, and that a change in network polarity correlates with behavioural change. Exploring the properties of the contractile network, we find that cell contractility, as determined by myosin activity, has an impact on the behaviour of the network, as well as on cytoskeletal architecture and cell behaviour. Pulsed contractions occur only in cells with intermediate levels of contractility. Furthermore, increasing levels of the small Rho GTPase Rho1 disrupts pulsing, leading to cells that cycle between two states, characterised by a junctional cortical and an apicomedial actin network. Our results highlight that behavioural change relies on tightly controlled cellular contractility. Moreover, we show that constriction can occur without pulsing, raising questions why constricting cells pulse in some contexts but not in others.
Close

Details

Original languageEnglish
Article numberjcs235325
Number of pages18
JournalJournal of Cell Science
Volume133
Issue number6
DOIs
Publication statusPublished - 30 Mar 2020

    Research areas

  • Cell migration, Apical constriction, Pulsed contractions, Actomyosin contractility, Drosophila

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells

    Baena-Lopez, L. A., Arthurton, L., Bischoff, M., Vincent, J-P., Alexandre, C. & McGregor, R., 4 Dec 2018, In: Development. 145, 23, 11 p., dev170811.

    Research output: Contribution to journalArticlepeer-review

  2. In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia

    Seijo, I., Guerrero, I. & Bischoff, M., 2015, Methods in Molecular Biology: Hedgehog Signalling Protocols. Riobo, N. (ed.). Springer Science and Business Media, Vol. 1322.

    Research output: Chapter in Book/Report/Conference proceedingChapter

  3. Exosomes as Hedgehog carriers in cytoneme-mediated transport and secretion

    Gradilla, A-C., González, E., Seijo, I., Andrés, G., Bischoff, M., González-Mendez, L., Sánchez, V., Callejo, A., Ibáñez, C., Guerra, M., Ortigão-Farias, J. R., Sutherland, J. D., González, M., Barrio, R., Falcón-Pérez, J. M. & Guerrero, I., 4 Dec 2014, In: Nature Communications. 5, 13 p., 5649.

    Research output: Contribution to journalArticlepeer-review

  4. Epidermal growth factor signalling controls Myosin II planar polarity to orchestrate convergent extension movements during Drosophila tubulogenesis

    Saxena, A., Denholm, B., Bunt, S., Bischoff, M., VijayRaghavan, K. & Skaer, H., 2 Dec 2014, In: PLoS Biology. 12, 12, 17 p., e1002013.

    Research output: Contribution to journalArticlepeer-review

  5. The conserved transmembrane proteoglycan Perdido/Kon-tiki is essential for myofibrillogenesis and sarcomeric structure in Drosophila

    Pérez-Moreno, J. J., Bischoff, M., Martín-Bermudo, M. D. & Estrada, B., Jul 2014, In: Journal of Cell Science. 127, p. 3162-3173

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Neurochondrin interacts with the SMN protein suggesting a novel mechanism for Spinal Muscular Atrophy pathology

    Thompson, L. W., Morrison, K. D., Shirran, S. L., Groen, E. J. N., Gillingwater, T. H., Botting, C. H. & Sleeman, J. E., 17 Apr 2018, In: Journal of Cell Science. 131, 8, 18 p., jcs211482.

    Research output: Contribution to journalArticlepeer-review

  2. P2Y(6) receptor inhibition perturbs CCL2-evoked signalling in human monocytic and peripheral blood mononuclear cells

    Campwala, H., Sexton, D. W., Crossman, D. C. & Fountain, S. J., 15 Nov 2014, In: Journal of Cell Science. 127, 22, p. 4964-4973 10 p.

    Research output: Contribution to journalArticlepeer-review

  3. The conserved transmembrane proteoglycan Perdido/Kon-tiki is essential for myofibrillogenesis and sarcomeric structure in Drosophila

    Pérez-Moreno, J. J., Bischoff, M., Martín-Bermudo, M. D. & Estrada, B., Jul 2014, In: Journal of Cell Science. 127, p. 3162-3173

    Research output: Contribution to journalArticlepeer-review

  4. Time-resolved quantitative proteomics implicates the core snRNP protein SmB together with SMN in neural trafficking

    Prescott, A. R., Bales, A., James, J., Trinkle-Mulcahy, L. & Sleeman, J. E., 14 Feb 2014, In: Journal of Cell Science. 127, 4, p. 812-827 15 p.

    Research output: Contribution to journalArticlepeer-review

ID: 265898135

Top