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Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

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Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis. / Pulido Companys, Pau; Norris, Anneliese; Bischoff, Marcus.

In: Journal of Cell Science, Vol. 133, No. 6, jcs235325, 30.03.2020.

Research output: Contribution to journalArticlepeer-review

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Pulido Companys, P, Norris, A & Bischoff, M 2020, 'Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis', Journal of Cell Science, vol. 133, no. 6, jcs235325. https://doi.org/10.1242/jcs.235325

APA

Pulido Companys, P., Norris, A., & Bischoff, M. (2020). Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis. Journal of Cell Science, 133(6), [jcs235325]. https://doi.org/10.1242/jcs.235325

Vancouver

Pulido Companys P, Norris A, Bischoff M. Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis. Journal of Cell Science. 2020 Mar 30;133(6). jcs235325. https://doi.org/10.1242/jcs.235325

Author

Pulido Companys, Pau ; Norris, Anneliese ; Bischoff, Marcus. / Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis. In: Journal of Cell Science. 2020 ; Vol. 133, No. 6.

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@article{b5d00b3bb00f43de9af2301d31e5d01c,
title = "Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis",
abstract = "During morphogenesis, cells exhibit various behaviours, such as migration and constriction, which need to be coordinated. How this is achieved remains elusive. During morphogenesis of the Drosophila adult abdominal epidermis, larval epithelial cells (LECs) migrate directedly before constricting apically and undergoing apoptosis. Here, we study the mechanisms underlying the transition from migration to constriction. We show that LECs possess a pulsatile apical actomyosin network, and that a change in network polarity correlates with behavioural change. Exploring the properties of the contractile network, we find that cell contractility, as determined by myosin activity, has an impact on the behaviour of the network, as well as on cytoskeletal architecture and cell behaviour. Pulsed contractions occur only in cells with intermediate levels of contractility. Furthermore, increasing levels of the small Rho GTPase Rho1 disrupts pulsing, leading to cells that cycle between two states, characterised by a junctional cortical and an apicomedial actin network. Our results highlight that behavioural change relies on tightly controlled cellular contractility. Moreover, we show that constriction can occur without pulsing, raising questions why constricting cells pulse in some contexts but not in others.",
keywords = "Cell migration, Apical constriction, Pulsed contractions, Actomyosin contractility, Drosophila",
author = "{Pulido Companys}, Pau and Anneliese Norris and Marcus Bischoff",
note = "This work was supported by the BBSRC (BB/M021084/1).",
year = "2020",
month = mar,
day = "30",
doi = "10.1242/jcs.235325",
language = "English",
volume = "133",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

AU - Pulido Companys, Pau

AU - Norris, Anneliese

AU - Bischoff, Marcus

N1 - This work was supported by the BBSRC (BB/M021084/1).

PY - 2020/3/30

Y1 - 2020/3/30

N2 - During morphogenesis, cells exhibit various behaviours, such as migration and constriction, which need to be coordinated. How this is achieved remains elusive. During morphogenesis of the Drosophila adult abdominal epidermis, larval epithelial cells (LECs) migrate directedly before constricting apically and undergoing apoptosis. Here, we study the mechanisms underlying the transition from migration to constriction. We show that LECs possess a pulsatile apical actomyosin network, and that a change in network polarity correlates with behavioural change. Exploring the properties of the contractile network, we find that cell contractility, as determined by myosin activity, has an impact on the behaviour of the network, as well as on cytoskeletal architecture and cell behaviour. Pulsed contractions occur only in cells with intermediate levels of contractility. Furthermore, increasing levels of the small Rho GTPase Rho1 disrupts pulsing, leading to cells that cycle between two states, characterised by a junctional cortical and an apicomedial actin network. Our results highlight that behavioural change relies on tightly controlled cellular contractility. Moreover, we show that constriction can occur without pulsing, raising questions why constricting cells pulse in some contexts but not in others.

AB - During morphogenesis, cells exhibit various behaviours, such as migration and constriction, which need to be coordinated. How this is achieved remains elusive. During morphogenesis of the Drosophila adult abdominal epidermis, larval epithelial cells (LECs) migrate directedly before constricting apically and undergoing apoptosis. Here, we study the mechanisms underlying the transition from migration to constriction. We show that LECs possess a pulsatile apical actomyosin network, and that a change in network polarity correlates with behavioural change. Exploring the properties of the contractile network, we find that cell contractility, as determined by myosin activity, has an impact on the behaviour of the network, as well as on cytoskeletal architecture and cell behaviour. Pulsed contractions occur only in cells with intermediate levels of contractility. Furthermore, increasing levels of the small Rho GTPase Rho1 disrupts pulsing, leading to cells that cycle between two states, characterised by a junctional cortical and an apicomedial actin network. Our results highlight that behavioural change relies on tightly controlled cellular contractility. Moreover, we show that constriction can occur without pulsing, raising questions why constricting cells pulse in some contexts but not in others.

KW - Cell migration

KW - Apical constriction

KW - Pulsed contractions

KW - Actomyosin contractility

KW - Drosophila

U2 - 10.1242/jcs.235325

DO - 10.1242/jcs.235325

M3 - Article

VL - 133

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 6

M1 - jcs235325

ER -

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