Skip to content

Research at St Andrews

CRISPR interference: a structural perspective

Research output: Contribution to journalReview article

DOI

Open Access permissions

Open

Author(s)

Judith Anne Reeks, Jim Naismith, Malcolm F White

School/Research organisations

Abstract

CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive defence system, providing immunity against mobile genetic elements such as viruses. Genomically encoded crRNA (CRISPR RNA) is used by Cas (CRISPR-associated) proteins to target and subsequently degrade nucleic acids of invading entities in a sequence-dependent manner. The process is known as ‘interference’. In the present review we cover recent progress on the structural biology of the CRISPR/Cas system, focusing on the Cas proteins and complexes that catalyse crRNA biogenesis and interference. Structural studies have helped in the elucidation of key mechanisms, including the recognition and cleavage of crRNA by the Cas6 and Cas5 proteins, where remarkable diversity at the level of both substrate recognition and catalysis has become apparent. The RNA-binding RAMP (repeat-associated mysterious protein) domain is present in the Cas5, Cas6, Cas7 and Cmr3 protein families and RAMP-like domains are found in Cas2 and Cas10. Structural analysis has also revealed an evolutionary link between the small subunits of the type I and type III-B interference complexes. Future studies of the interference complexes and their constituent components will transform our understanding of the system.
Close

Details

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalBiochemical Journal
Volume453
Issue number2
DOIs
Publication statusPublished - Jun 2013

    Research areas

  • Antiviral defence, Cluster of regularly interspaced palindromic repeats (CRISPR), Crystallography, Evolution, Protein structure, Repeat-associated mysterious protein (RAMP)

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. A type III CRISPR ancillary ribonuclease degrades its cyclic oligoadenylate activator

    Athukoralage, J. S., Graham, S., Grüschow, S., Rouillon, C. & White, M. F., 12 Jul 2019, In : Journal of Molecular Biology. 431, 15, p. 2894-2899

    Research output: Contribution to journalArticle

  2. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems

    Rouillon, C., Athukoralage, J. S., Graham, S., Grüschow, S. & White, M. F., 1 Jan 2019, Methods in Enzymology. Bailey, S. (ed.). Academic Press Inc., p. 191-218 28 p. (Methods in Enzymology; vol. 616).

    Research output: Chapter in Book/Report/Conference proceedingChapter

  3. Ring nucleases deactivate Type III CRISPR ribonucleases by degrading cyclic oligoadenylate

    Athukoralage, J. S., Rouillon, C., Graham, S., Grüschow, S. & White, M. F., 11 Oct 2018, In : Nature. 562, 7726, p. 277-280 16 p.

    Research output: Contribution to journalArticle

  4. Control of cyclic oligoadenylate synthesis in a type III CRISPR system

    Rouillon, C., Athukoralage, J. S., Graham, S., Grüschow, S. & White, M. F., 19 Jul 2018, In : eLife. 7, 22 p., e36734.

    Research output: Contribution to journalArticle

  5. DNA repair in the Archaea – an emerging picture

    White, M. F. & Allers, T., Jul 2018, In : FEMS Microbiology Reviews. 42, 4, p. 514-526 13 p.

    Research output: Contribution to journalReview article

Related by journal

  1. Biochemical Journal (Journal)

    Terry K Smith (Member of editorial board)
    2013 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  2. Biochemical Journal (Journal)

    Malcolm F White (Editor)
    May 2010 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  3. Biochemical Journal (Journal)

    Malcolm F White (Member of editorial board)
    2008 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  4. Biochemical Journal (Journal)

    Jim Naismith (Editor)
    20032004

    Activity: Publication peer-review and editorial work typesEditor of research journal

Related by journal

  1. The hydrophobic region of the Leishmania peroxin 14: requirements for association with a glycosome mimetic membrane

    Cyr, N., Smith, T. K., Boisselier, É., Leroux, L-P., Kottarampatel, A. H., Davidsen, A., Salesse, C. & Jardim, A., 31 Jan 2018, In : Biochemical Journal. 475, 2, p. 511-529 19 p.

    Research output: Contribution to journalArticle

  2. Physiological and pathophysiological functions of Swiprosin-1/EFhd2 in the nervous system

    Mielenz, D. & Gunn-Moore, F. J., 15 Aug 2016, In : Biochemical Journal. 473, 16, p. 2429-2437

    Research output: Contribution to journalReview article

  3. The structural basis of DNA binding by the single-stranded DNA-binding protein from Sulfolobus solfataricus

    Gamsjaeger, R., Kariawasam, R., Gimenez, A. X., Touma, C., McIlwain, E., Bernardo, R. E., Shepherd, N. E., Ataide, S. F., Dong, Q., Richard, D. J., White, M. F. & Cubeddu, L., 15 Jan 2015, In : Biochemical Journal. 465, 2, p. 337-346 10 p.

    Research output: Contribution to journalArticle

ID: 60328306