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Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi

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Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi. / Fauzi, Muh; Kim, Namhyeok; Nyunt, Myat Htut; Firdaus, Egy Rahman; Han, Jin-Hee; Hoque, Mohammed Rafiul; Lee, Seong-Kyun; Park, Ji-Hoon; Moon, Robert W.; Lau, Yee Ling; Kaneko, Osamu; Han, Eun-Taek.

In: PLoS Neglected Tropical Diseases, Vol. 14, No. 6, e0008323, 19.06.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Fauzi, M, Kim, N, Nyunt, MH, Firdaus, ER, Han, J-H, Hoque, MR, Lee, S-K, Park, J-H, Moon, RW, Lau, YL, Kaneko, O & Han, E-T 2020, 'Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi', PLoS Neglected Tropical Diseases, vol. 14, no. 6, e0008323. https://doi.org/10.1371/journal.pntd.0008323

APA

Fauzi, M., Kim, N., Nyunt, M. H., Firdaus, E. R., Han, J-H., Hoque, M. R., Lee, S-K., Park, J-H., Moon, R. W., Lau, Y. L., Kaneko, O., & Han, E-T. (2020). Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi. PLoS Neglected Tropical Diseases, 14(6), [e0008323]. https://doi.org/10.1371/journal.pntd.0008323

Vancouver

Fauzi M, Kim N, Nyunt MH, Firdaus ER, Han J-H, Hoque MR et al. Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi. PLoS Neglected Tropical Diseases. 2020 Jun 19;14(6). e0008323. https://doi.org/10.1371/journal.pntd.0008323

Author

Fauzi, Muh ; Kim, Namhyeok ; Nyunt, Myat Htut ; Firdaus, Egy Rahman ; Han, Jin-Hee ; Hoque, Mohammed Rafiul ; Lee, Seong-Kyun ; Park, Ji-Hoon ; Moon, Robert W. ; Lau, Yee Ling ; Kaneko, Osamu ; Han, Eun-Taek. / Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi. In: PLoS Neglected Tropical Diseases. 2020 ; Vol. 14, No. 6.

Bibtex - Download

@article{265b906e07284c27bfe29b258e6a37ee,
title = "Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi",
abstract = "Malaria is caused by multiple different species of protozoan parasites, and interventions in the pre-elimination phase can lead to drastic changes in the proportion of each species causing malaria. In endemic areas, cross-reactivity may play an important role in the protection and blocking transmission. Thus, successful control of one species could lead to an increase in other parasite species. A few studies have reported cross-reactivity producing cross-immunity, but the extent of cross-reactive, particularly between closely related species, is poorly understood. P. vivax and P. knowlesi are particularly closely related species causing malaria infections in SE Asia, and whilst P. vivax cases are in decline, zoonotic P. knowlesi infections are rising in some areas. In this study, the cross-species reactivity and growth inhibition activity of P. vivax blood-stage antigen-specific antibodies against P. knowlesi parasites were investigated. Bioinformatics analysis, immunofluorescence assay, western blotting, protein microarray, and growth inhibition assay were performed to investigate the cross-reactivity. P. vivax blood-stage antigen-specific antibodies recognized the molecules located on the surface or released from apical organelles of P. knowlesi merozoites. Recombinant P. vivax and P. knowlesi proteins were also recognized by P. knowlesi- and P. vivax-infected patient antibodies, respectively. Immunoglobulin G against P. vivax antigens from both immune animals and human malaria patients inhibited the erythrocyte invasion by P. knowlesi. This study demonstrates that there is extensive cross-reactivity between antibodies against P. vivax to P. knowlesi in the blood stage, and these antibodies can potently inhibit in vitro invasion, highlighting the potential cross-protective immunity in endemic areas.",
author = "Muh Fauzi and Namhyeok Kim and Nyunt, {Myat Htut} and Firdaus, {Egy Rahman} and Jin-Hee Han and Hoque, {Mohammed Rafiul} and Seong-Kyun Lee and Ji-Hoon Park and Moon, {Robert W.} and Lau, {Yee Ling} and Osamu Kaneko and Eun-Taek Han",
note = "Funding: FM would like to thank Indonesia Government Fund for Education for the financial support of his scholarship. This study was supported by Indonesia Government Fund for Education (LPDP/20140812021475) (FM), by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2017R1A2A2A05069562) (ETH), by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015R1A4A1038666) (ETH). RM is supported by a UK Medical Research Council Career Development Award Fellowship (MR/M021157/1).",
year = "2020",
month = jun,
day = "19",
doi = "10.1371/journal.pntd.0008323",
language = "English",
volume = "14",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2735",
publisher = "Public Library of Science",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi

AU - Fauzi, Muh

AU - Kim, Namhyeok

AU - Nyunt, Myat Htut

AU - Firdaus, Egy Rahman

AU - Han, Jin-Hee

AU - Hoque, Mohammed Rafiul

AU - Lee, Seong-Kyun

AU - Park, Ji-Hoon

AU - Moon, Robert W.

AU - Lau, Yee Ling

AU - Kaneko, Osamu

AU - Han, Eun-Taek

N1 - Funding: FM would like to thank Indonesia Government Fund for Education for the financial support of his scholarship. This study was supported by Indonesia Government Fund for Education (LPDP/20140812021475) (FM), by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2017R1A2A2A05069562) (ETH), by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015R1A4A1038666) (ETH). RM is supported by a UK Medical Research Council Career Development Award Fellowship (MR/M021157/1).

PY - 2020/6/19

Y1 - 2020/6/19

N2 - Malaria is caused by multiple different species of protozoan parasites, and interventions in the pre-elimination phase can lead to drastic changes in the proportion of each species causing malaria. In endemic areas, cross-reactivity may play an important role in the protection and blocking transmission. Thus, successful control of one species could lead to an increase in other parasite species. A few studies have reported cross-reactivity producing cross-immunity, but the extent of cross-reactive, particularly between closely related species, is poorly understood. P. vivax and P. knowlesi are particularly closely related species causing malaria infections in SE Asia, and whilst P. vivax cases are in decline, zoonotic P. knowlesi infections are rising in some areas. In this study, the cross-species reactivity and growth inhibition activity of P. vivax blood-stage antigen-specific antibodies against P. knowlesi parasites were investigated. Bioinformatics analysis, immunofluorescence assay, western blotting, protein microarray, and growth inhibition assay were performed to investigate the cross-reactivity. P. vivax blood-stage antigen-specific antibodies recognized the molecules located on the surface or released from apical organelles of P. knowlesi merozoites. Recombinant P. vivax and P. knowlesi proteins were also recognized by P. knowlesi- and P. vivax-infected patient antibodies, respectively. Immunoglobulin G against P. vivax antigens from both immune animals and human malaria patients inhibited the erythrocyte invasion by P. knowlesi. This study demonstrates that there is extensive cross-reactivity between antibodies against P. vivax to P. knowlesi in the blood stage, and these antibodies can potently inhibit in vitro invasion, highlighting the potential cross-protective immunity in endemic areas.

AB - Malaria is caused by multiple different species of protozoan parasites, and interventions in the pre-elimination phase can lead to drastic changes in the proportion of each species causing malaria. In endemic areas, cross-reactivity may play an important role in the protection and blocking transmission. Thus, successful control of one species could lead to an increase in other parasite species. A few studies have reported cross-reactivity producing cross-immunity, but the extent of cross-reactive, particularly between closely related species, is poorly understood. P. vivax and P. knowlesi are particularly closely related species causing malaria infections in SE Asia, and whilst P. vivax cases are in decline, zoonotic P. knowlesi infections are rising in some areas. In this study, the cross-species reactivity and growth inhibition activity of P. vivax blood-stage antigen-specific antibodies against P. knowlesi parasites were investigated. Bioinformatics analysis, immunofluorescence assay, western blotting, protein microarray, and growth inhibition assay were performed to investigate the cross-reactivity. P. vivax blood-stage antigen-specific antibodies recognized the molecules located on the surface or released from apical organelles of P. knowlesi merozoites. Recombinant P. vivax and P. knowlesi proteins were also recognized by P. knowlesi- and P. vivax-infected patient antibodies, respectively. Immunoglobulin G against P. vivax antigens from both immune animals and human malaria patients inhibited the erythrocyte invasion by P. knowlesi. This study demonstrates that there is extensive cross-reactivity between antibodies against P. vivax to P. knowlesi in the blood stage, and these antibodies can potently inhibit in vitro invasion, highlighting the potential cross-protective immunity in endemic areas.

U2 - 10.1371/journal.pntd.0008323

DO - 10.1371/journal.pntd.0008323

M3 - Article

VL - 14

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2735

IS - 6

M1 - e0008323

ER -

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