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Crumbs 3b promotes tight junctions in an ezrin-dependent manner in mammalian cells

Research output: Contribution to journalArticle

Author(s)

Andrew Martin Tilston-Lunel, Kathryn Haley, Nicolas Schlecht, Yanhua Wang, Abigail Louise Dougal Chatterton, Susana Luisa Moleirinho, Ailsa Watson, Harinder Hundal, Michael Prystowsky, Francis James Gunn-Moore, Paul Andrew Reynolds

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Abstract

Crumbs3 (CRB3) is a component of epithelial junctions that has been implicated in apical-basal polarity, apical identity, apical stability, cell adhesion and cell growth. CRB3 undergoes alternative splicing to yield two variants: CRB3a and CRB3b. Here, we describe novel data demonstrating that as with previous studies on CRB3a, CRB3b also promotes the formation of tight junctions. However, significantly we demonstrate that the 4.1-ezrin-radixin-moesin (FERM) binding motif (FBM) of CRB3b is required for CRB3b functionality and that ezrin binds to the FBM of CRB3b. Furthermore, we show that ezrin contributes to CRB3b functionality and the correct distribution of tight junction proteins. We demonstrate that both CRB3 isoforms are required for the production of functionally mature tight junctions and also the localization of ezrin to the plasma membrane. Finally, we demonstrate that reduced CRB3b expression in head and neck squamous cell carcinoma (HNSCC) correlates with cytoplasmic ezrin, a biomarker for aggressive disease, and show evidence that whilst CRB3a expression has no effect, low CRB3b and high cytoplasmic ezrin expression combined may be prognostic for HNSCC.
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Details

Original languageEnglish
Pages (from-to)439-455
JournalJournal of Molecular Cell Biology
Volume8
Issue number5
Early online date18 Apr 2016
DOIs
Publication statusPublished - Oct 2016

    Research areas

  • CRB3, Crumbs, FERM proteins, FBM, Tight junctions, Ezrin

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ID: 240593744

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