Skip to content

Research at St Andrews

Cytoadherence and virulence - the case of Plasmodium knowlesi malaria

Research output: Contribution to journalArticle

Standard

Cytoadherence and virulence - the case of Plasmodium knowlesi malaria. / Fatih, Farrah A.; Siner, Angela; Ahmed, Atique; Woon, Lu Chan; Craig, Alister G.; Singh, Balbir; Krishna, Sanjeev; Cox Singh, Janet.

In: Malaria Journal, Vol. 11, 33, 03.02.2012.

Research output: Contribution to journalArticle

Harvard

Fatih, FA, Siner, A, Ahmed, A, Woon, LC, Craig, AG, Singh, B, Krishna, S & Cox Singh, J 2012, 'Cytoadherence and virulence - the case of Plasmodium knowlesi malaria', Malaria Journal, vol. 11, 33. https://doi.org/10.1186/1475-2875-11-33

APA

Fatih, F. A., Siner, A., Ahmed, A., Woon, L. C., Craig, A. G., Singh, B., ... Cox Singh, J. (2012). Cytoadherence and virulence - the case of Plasmodium knowlesi malaria. Malaria Journal, 11, [33]. https://doi.org/10.1186/1475-2875-11-33

Vancouver

Fatih FA, Siner A, Ahmed A, Woon LC, Craig AG, Singh B et al. Cytoadherence and virulence - the case of Plasmodium knowlesi malaria. Malaria Journal. 2012 Feb 3;11. 33. https://doi.org/10.1186/1475-2875-11-33

Author

Fatih, Farrah A. ; Siner, Angela ; Ahmed, Atique ; Woon, Lu Chan ; Craig, Alister G. ; Singh, Balbir ; Krishna, Sanjeev ; Cox Singh, Janet. / Cytoadherence and virulence - the case of Plasmodium knowlesi malaria. In: Malaria Journal. 2012 ; Vol. 11.

Bibtex - Download

@article{3e6c78ba58dc4f4ba7332dfa46d0adca,
title = "Cytoadherence and virulence - the case of Plasmodium knowlesi malaria",
abstract = "Background: Cytoadherence of infected red blood cells to brain endothelium is causally implicated in malarial coma, one of the severe manifestations of falciparum malaria. Cytoadherence is mediated by specific binding of variant parasite antigens, expressed on the surface of infected erythrocytes, to endothelial receptors including, ICAM-1, VCAM and CD36. In fatal cases of severe falciparum malaria with coma, blood vessels in the brain are characteristically congested with infected erythrocytes. Brain sections from a fatal case of knowlesi malaria, but without coma, were similarly congested with infected erythrocytes. The objective of this study was to determine the binding phenotype of Plasmodium knowlesi infected human erythrocytes to recombinant human ICAM-1, VCAM and CD36.Methods: Five patients with PCR-confirmed P. knowlesi malaria were recruited into the study with consent between April and August 2010. Pre-treatment venous blood was washed and cultured ex vivo to increase the proportion of schizont-infected erythrocytes. Cultured blood was seeded into Petri dishes with triplicate areas coated with ICAM-1, VCAM and CD36. Following incubation at 37 degrees C for one hour the dishes were washed and the number of infected erythrocytes bound/mm(2) to PBS control areas and to recombinant human ICAM-1 VCAM and CD36 coated areas were recorded. Each assay was performed in duplicate. Assay performance was monitored with the Plasmodium falciparum clone HB3.Results: Blood samples were cultured ex vivo for up to 14.5 h (mean 11.3 +/- 1.9 h) to increase the relative proportion of mature trophozoite and schizont-infected red blood cells to at least 50{\%} (mean 65.8 +/- 17.51{\%}). Three (60{\%}) isolates bound significantly to ICAM-1 and VCAM, one (20{\%}) isolate bound to VCAM and none of the five bound significantly to CD36.Conclusions: Plasmodium knowlesi infected erythrocytes from human subjects bind in a specific but variable manner to the inducible endothelial receptors ICAM-1 and VCAM. Binding to the constitutively-expressed endothelial receptor CD36 was not detected. Further work will be required to define the pathological consequences of these interactions.",
author = "Fatih, {Farrah A.} and Angela Siner and Atique Ahmed and Woon, {Lu Chan} and Craig, {Alister G.} and Balbir Singh and Sanjeev Krishna and {Cox Singh}, Janet",
year = "2012",
month = "2",
day = "3",
doi = "10.1186/1475-2875-11-33",
language = "English",
volume = "11",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cytoadherence and virulence - the case of Plasmodium knowlesi malaria

AU - Fatih, Farrah A.

AU - Siner, Angela

AU - Ahmed, Atique

AU - Woon, Lu Chan

AU - Craig, Alister G.

AU - Singh, Balbir

AU - Krishna, Sanjeev

AU - Cox Singh, Janet

PY - 2012/2/3

Y1 - 2012/2/3

N2 - Background: Cytoadherence of infected red blood cells to brain endothelium is causally implicated in malarial coma, one of the severe manifestations of falciparum malaria. Cytoadherence is mediated by specific binding of variant parasite antigens, expressed on the surface of infected erythrocytes, to endothelial receptors including, ICAM-1, VCAM and CD36. In fatal cases of severe falciparum malaria with coma, blood vessels in the brain are characteristically congested with infected erythrocytes. Brain sections from a fatal case of knowlesi malaria, but without coma, were similarly congested with infected erythrocytes. The objective of this study was to determine the binding phenotype of Plasmodium knowlesi infected human erythrocytes to recombinant human ICAM-1, VCAM and CD36.Methods: Five patients with PCR-confirmed P. knowlesi malaria were recruited into the study with consent between April and August 2010. Pre-treatment venous blood was washed and cultured ex vivo to increase the proportion of schizont-infected erythrocytes. Cultured blood was seeded into Petri dishes with triplicate areas coated with ICAM-1, VCAM and CD36. Following incubation at 37 degrees C for one hour the dishes were washed and the number of infected erythrocytes bound/mm(2) to PBS control areas and to recombinant human ICAM-1 VCAM and CD36 coated areas were recorded. Each assay was performed in duplicate. Assay performance was monitored with the Plasmodium falciparum clone HB3.Results: Blood samples were cultured ex vivo for up to 14.5 h (mean 11.3 +/- 1.9 h) to increase the relative proportion of mature trophozoite and schizont-infected red blood cells to at least 50% (mean 65.8 +/- 17.51%). Three (60%) isolates bound significantly to ICAM-1 and VCAM, one (20%) isolate bound to VCAM and none of the five bound significantly to CD36.Conclusions: Plasmodium knowlesi infected erythrocytes from human subjects bind in a specific but variable manner to the inducible endothelial receptors ICAM-1 and VCAM. Binding to the constitutively-expressed endothelial receptor CD36 was not detected. Further work will be required to define the pathological consequences of these interactions.

AB - Background: Cytoadherence of infected red blood cells to brain endothelium is causally implicated in malarial coma, one of the severe manifestations of falciparum malaria. Cytoadherence is mediated by specific binding of variant parasite antigens, expressed on the surface of infected erythrocytes, to endothelial receptors including, ICAM-1, VCAM and CD36. In fatal cases of severe falciparum malaria with coma, blood vessels in the brain are characteristically congested with infected erythrocytes. Brain sections from a fatal case of knowlesi malaria, but without coma, were similarly congested with infected erythrocytes. The objective of this study was to determine the binding phenotype of Plasmodium knowlesi infected human erythrocytes to recombinant human ICAM-1, VCAM and CD36.Methods: Five patients with PCR-confirmed P. knowlesi malaria were recruited into the study with consent between April and August 2010. Pre-treatment venous blood was washed and cultured ex vivo to increase the proportion of schizont-infected erythrocytes. Cultured blood was seeded into Petri dishes with triplicate areas coated with ICAM-1, VCAM and CD36. Following incubation at 37 degrees C for one hour the dishes were washed and the number of infected erythrocytes bound/mm(2) to PBS control areas and to recombinant human ICAM-1 VCAM and CD36 coated areas were recorded. Each assay was performed in duplicate. Assay performance was monitored with the Plasmodium falciparum clone HB3.Results: Blood samples were cultured ex vivo for up to 14.5 h (mean 11.3 +/- 1.9 h) to increase the relative proportion of mature trophozoite and schizont-infected red blood cells to at least 50% (mean 65.8 +/- 17.51%). Three (60%) isolates bound significantly to ICAM-1 and VCAM, one (20%) isolate bound to VCAM and none of the five bound significantly to CD36.Conclusions: Plasmodium knowlesi infected erythrocytes from human subjects bind in a specific but variable manner to the inducible endothelial receptors ICAM-1 and VCAM. Binding to the constitutively-expressed endothelial receptor CD36 was not detected. Further work will be required to define the pathological consequences of these interactions.

U2 - 10.1186/1475-2875-11-33

DO - 10.1186/1475-2875-11-33

M3 - Article

VL - 11

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

M1 - 33

ER -

Related by author

  1. Plasmodium knowlesi: experimental model, zoonotic pathogen and golden opportunity?

    Cox-Singh, J., Jan 2018, In : Parasitology. 145, 1, p. 1-5 4 p.

    Research output: Contribution to journalEditorial

  2. Human infections with Plasmodium knowlesi - zoonotic malaria

    Millar, S. B. & Cox Singh, J., Jul 2015, In : Clinical Microbiology and Infection. 21, 7, p. 640-648

    Research output: Contribution to journalArticle

  3. Plasmodium knowlesi: from severe zoonosis to animal model

    Cox Singh, J. & Culleton, R., Jun 2015, In : Trends in Parasitology. 31, 6

    Research output: Contribution to journalArticle

  4. Plasmodium knowlesi genome sequences from clinical isolates reveal extensive genomic dimorphism

    Monsanto Pinheiro, M., Ahmed, M. A., Millar, S. B., Sanderson, T., Otto, T. D., Lu, W. C., Krishna, S., Rayner, J. C. & Cox-Singh, J., 1 Apr 2015, In : PLoS One. 10, 4, 16 p., e0121303.

    Research output: Contribution to journalArticle

  5. Plasmodium knowlesi – an emerging pathogen

    Ahmed, M. A. & Cox Singh, J., Apr 2015, In : ISBT Science Series. 10, S1, p. 134-140

    Research output: Contribution to journalReview article

Related by journal

  1. The acquisition of long-lived memory B cell responses to merozoite surface protein-8 in individuals with Plasmodium vivax infection

    Kochayoo, P., Kittisenachai, N., Changrob, S., Wangriatisak, K., Muh, F., Chootong, P. & Han, E-T., 31 May 2019, In : Malaria Journal. 18, 10 p., 188.

    Research output: Contribution to journalArticle

  2. Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan

    Hoque, M. R., Elfaki, M. M. A., Ahmed, M. A., Lee, S-K., Muh, F., Albsheer, M. M. A., Hamid, M. M. A. & Han, E-T., 17 Aug 2018, In : Malaria Journal. 17, 10 p., 297.

    Research output: Contribution to journalArticle

  3. Estimation on local transmission of malaria by serological approach under low transmission setting in Myanmar

    Nyunt, M. H., Soe, T. N., Shein, T., Zaw, N. N., Han, S. S., Muh, F., Lee, S-K., Han, J-H., Park, J-H., Ha, K-S., Park, W. S., Hong, S-H., Kyaw, M. P. & Han, E-T., 5 Jan 2018, In : Malaria Journal. 17, 9 p., 6.

    Research output: Contribution to journalArticle

  4. Genetic diversity and natural selection of Plasmodium knowlesi merozoite surface protein 1 paralog gene in Malaysia

    Ahmed, M. A., Fauzi, M. & Han, E-T., 14 Mar 2018, In : Malaria Journal. 17, 11 p., 115.

    Research output: Contribution to journalArticle

  5. In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strain

    Muh, F., Lee, S-K., Hoque, M. R., Han, J-H., Park, J-H., Firdaus, E. R., Moon, R. W., Lau, Y. L. & Han, E-T., 27 Jul 2018, In : Malaria Journal. 17, 11 p., 272.

    Research output: Contribution to journalArticle

ID: 23164689

Top