Skip to content

Research at St Andrews

Deep sequencing analysis of defective genomes of parainfluenza virus 5 and their role in interferon induction

Research output: Contribution to journalArticlepeer-review

DOI

Open Access permissions

Open

Author(s)

M. J. Killip, D. F. Young, D. Gatherer, C. S. Ross, J. A. L. Short, A. J. Davison, S. Goodbourn, R. E. Randall

School/Research organisations

Abstract

Preparations of parainfluenza virus 5 (PIV5) that are potent activators of the interferon (IFN) induction cascade were generated by high-multiplicity passage in order to accumulate defective interfering virus genomes (DIs). Nucleocapsid RNA from these virus preparations was extracted and subjected to deep sequencing. Sequencing data were analyzed using methods designed to detect internal deletion and "copyback" DIs in order to identify and characterize the different DIs present and to approximately quantify the ratio of defective to nondefective genomes. Trailer copybacks dominated the DI populations in IFN-inducing preparations of both the PIV5 wild type (wt) and PIV5-V Delta C (a recombinant virus that does not encode a functional V protein). Although the PIV5 V protein is an efficient inhibitor of the IFN induction cascade, we show that nondefective PIV5 wt is unable to prevent activation of the IFN response by coinfecting copyback DIs due to the interfering effects of copyback DIs on nondefective virus protein expression. As a result, copyback DIs are able to very rapidly activate the IFN induction cascade prior to the expression of detectable levels of V protein by coinfecting nondefective virus.

Close

Details

Original languageEnglish
Pages (from-to)4798-4807
Number of pages10
JournalJournal of Virology
Volume87
Issue number9
DOIs
Publication statusPublished - May 2013

    Research areas

  • Activation, Infected-cells, RIG-I, Paramyxovirus V proteins, Internal deletions, 5'-triphosphate RNA, IFN-Beta promoter, Viral-RNA Synthesis, Negative-strand virus, Antiviral responses

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Genetic lesions of type I interferon signalling in human antiviral immunity

    Duncan, C. J. A., Randall, R. E. & Hambleton, S., Jan 2021, In: Trends in Genetics. 37, 1, p. 46-58 13 p.

    Research output: Contribution to journalReview articlepeer-review

  2. Direct antiviral activity of interferon stimulated genes is responsible for resistance to paramyxoviruses in ISG15-deficient cells

    Holthaus, D., Vasou, A., Bamford, C., Andrejeva, J., Paulus, C., Randall, R. E., McLauchlan, J. & Hughes, D. J., 22 Jun 2020, In: The Journal of Immunology. 11 p., ji1901472.

    Research output: Contribution to journalArticlepeer-review

  3. Innate intracellular antiviral responses restrict the amplification of defective virus genomes of parainfluenza virus type 5

    Wignall-Fleming, E. B., Vasou, A., Young, D., Short, J. A. L., Hughes, D. J., Goodbourn, S. & Randall, R. E., 16 Jun 2020, In: Journal of Virology. 94, 13, 15 p., 00246-20.

    Research output: Contribution to journalArticlepeer-review

  4. Severe type I interferonopathy and unrestrained interferon signaling due to a homozygous germline mutation in STAT2

    Duncan, C. J. A., Thompson, B. J., Chen, R., Rice, G. I., Gothe, F., Young, D. F., Lovell, S. C., Shuttleworth, V. G., Brocklebank, V., Corner, B., Skelton, A. J., Bondet, V., Coxhead, J., Duffy, D., Fourrage, C., Livingston, J. H., Pavaine, J., Cheesman, E., Bitetti, S., Grainger, A. & 18 others, Acres, M., Innes, B. A., Mikulasova, A., Sun, R., Hussain, R., Wright, R., Wynn, R., Zarhrate, M., Zeef, L. A. H., Wood, K., Hughes, S. M., Harris, C. L., Engelhardt, K. R., Crow, Y. J., Randall, R. E., Kavanagh, D., Hambleton, S. & Briggs, T. A., 13 Dec 2019, In: Science Immunology. 4, 42, eaav7501.

    Research output: Contribution to journalArticlepeer-review

  5. Analysis of paramyxovirus transcription and replication by high-throughput sequencing

    Wignall-Fleming, E. B., Hughes, D. J., Vattipally, S., Modha, S., Goodbourn, S., Davison, A. J. & Randall, R. E., 13 Aug 2019, In: Journal of Virology. 93, 17, 17 p., e00571-19.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Journal of Virology (Journal)

    Richard Michael Elliott (Editor)

    20052013

    Activity: Publication peer-review and editorial work typesEditor of research journal

Related by journal

  1. Innate intracellular antiviral responses restrict the amplification of defective virus genomes of parainfluenza virus type 5

    Wignall-Fleming, E. B., Vasou, A., Young, D., Short, J. A. L., Hughes, D. J., Goodbourn, S. & Randall, R. E., 16 Jun 2020, In: Journal of Virology. 94, 13, 15 p., 00246-20.

    Research output: Contribution to journalArticlepeer-review

  2. The BHLF1 locus of Epstein-Barr virus contributes to viral latency and B-cell immortalization

    Yetming, K. D., Lupey-Green, L. N., Biryukov, S., Hughes, D. J., Marendy, E. M., Miranda, J. J. L. & Sample, J. T., 24 Jun 2020, In: Journal of Virology. Latest Articles, JVI01215-20.

    Research output: Contribution to journalArticlepeer-review

  3. Analysis of paramyxovirus transcription and replication by high-throughput sequencing

    Wignall-Fleming, E. B., Hughes, D. J., Vattipally, S., Modha, S., Goodbourn, S., Davison, A. J. & Randall, R. E., 13 Aug 2019, In: Journal of Virology. 93, 17, 17 p., e00571-19.

    Research output: Contribution to journalArticlepeer-review

  4. Human cytomegalovirus immediate early 1 protein causes loss of SOX2 from neural progenitor cells by trapping unphosphorylated STAT3 in the nucleus

    Wu, C-C., Jiang, X., Wang, X-Z., Liu, X-J., Li, X-J., Yang, B., Ye, H-Q., Harwardt, T., Jiang, M., Xia, H-M., Wang, W., Britt, W. J., Paulus, C., Nevels, M. M. & Luo, M-H., Sep 2018, In: Journal of Virology. 92, 17, e00340-18.

    Research output: Contribution to journalArticlepeer-review

  5. Association of human papillomavirus 16 E2 with Rad50-interacting protein 1 enhances viral DNA replication

    Campos-León, K., Wijendra, K., Siddiqa, A., Pentland, I., Feeney, K. M., Knapman, A., Davies, R., Androphy, E. J. & Parish, J. L., Mar 2017, In: Journal of Virology. 91, 5, 18 p., e02305-16.

    Research output: Contribution to journalArticlepeer-review

ID: 53493229

Top