Skip to content

Research at St Andrews

Defining the genes for the final steps in biosynthesis of the complex polyketide antibiotic mupirocin by Pseudomonas fluorescens NCIMB10586

Research output: Contribution to journalArticle

Author(s)

Jack A. Connolly, Amber Wilson, Malgorzata Macioszek, Zhongshu Song, Luoyi Wang, Hadi H. Mohammad, Mukul Yadav, Maura di Martino, Claire E. Miller, Joanne Hothersall, Anthony S. Haines, Elton R. Stephens, Matthew P. Crump, Christine L. Willis, Thomas J. Simpson, Peter J. Winn, Christopher M. Thomas

School/Research organisations

Abstract

The mupirocin trans-AT polyketide synthase pathway, provides a model system for manipulation of antibiotic biosynthesis. Its final phase involves removal of the tertiary hydroxyl group from pseudomonic acid B, PA-B, producing the fully active PA-A in a complex series of steps. To further clarify requirements for this conversion, we fed extracts containing PA-B to mutants of the producer strain singly deficient in each mup gene. This additionally identified mupM and mupN as required plus the sequence but not enzymic activity of mupL and ruled out need for other mup genes. A plasmid expressing mupLMNOPVCFU + macpE together with a derivative of the producer P. fluorescens strain NCIMB10586 lacking the mup cluster allowed conversion of PA-B to PA-A. MupN converts apo-mAcpE to holo-form while MupM is a mupirocin-resistant isoleucyl tRNA synthase, preventing self-poisoning. Surprisingly, the expression plasmid failed to allow the closely related P. fluorescens strain SBW25 to convert PA-B to PA-A.
Close

Details

Original languageEnglish
Article number1542
Number of pages9
JournalScientific Reports
Volume9
Early online date7 Feb 2019
DOIs
Publication statusE-pub ahead of print - 7 Feb 2019

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by journal

  1. Scientific Reports (Journal)

    Monica Arso Civil (Reviewer)
    Jul 2018 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. Scientific Reports (Journal)

    Anouk Margaretha Borst (Reviewer)
    10 Feb 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. Scientific Reports (Journal)

    Emma Louise Carroll (Member of editorial board)
    3 Oct 2016

    Activity: Publication peer-review and editorial work typesEditor of research journal

  4. Scientific Reports (Journal)

    Uli Schwarz-Linek (Reviewer)
    11 May 2016

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  5. Scientific Reports (Journal)

    Ildiko Maureen Lara Somorjai (Reviewer)
    2016 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. A comparative analysis of response times shows that multisensory benefits and interactions are not equivalent

    Innes, B. R. & Otto, T. U., 27 Feb 2019, In : Scientific Reports. 9, 10 p., 2921.

    Research output: Contribution to journalArticle

  2. A large explosive silicic eruption in the British Palaeogene Igneous Province

    Troll, V. R., Emeleus, C. H., Nicoll, G. R., Mattsson, T., Ellam, R. M., Donaldson, C. H. & Harris, C., 24 Jan 2019, In : Scientific Reports. 9, 1, 15 p., 494.

    Research output: Contribution to journalArticle

  3. Automated tumour budding quantification by machine learning augments TNM staging in muscle-invasive bladder cancer prognosis

    Brieu, N., Gavriel, C., Nearchou, I. P., Harrison, D. J., Schmidt, G. & Caie, P. D., 26 Mar 2019, In : Scientific Reports. 9, 5174.

    Research output: Contribution to journalArticle

  4. Classifying grey seal behaviour in relation to environmental variability and commercial fishing activity - a multivariate hidden Markov model

    van Beest, F. M., Mews, S., Elkenkamp, S., Schuhmann, P., Tsolak, D., Wobbe, T., Bartolino, V., Bastardie, F., Dietz, R., von Dorrien, C., Galatius, A., Karlsson, O., McConnell, B., Nabe-Nielsen, J., Olsen, M. T., Teilmann, J. & Langrock, R., 4 Apr 2019, In : Scientific Reports. 9, 14 p., 5642.

    Research output: Contribution to journalArticle

ID: 257793123