Skip to content

Research at St Andrews

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.
Close

Details

Original languageEnglish
Title of host publicationAntibiotic Resistance Protocols
EditorsStephen Gillespie
Place of PublicationNew York, NY
PublisherHumana Press/Springer
Pages41-50
Number of pages10
ISBN (Electronic)9781493976386
ISBN (Print)9781493976362
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1736
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

    Research areas

  • Raman spectroscopy, Mycobacteria, Phenotypic resistance, Lipids

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease

    Baron, V., Chen, M., Clark, S. O., Williams, A., Hammond, R. J. H., Dholakia, K. & Gillespie, S. H., 29 Aug 2017, In : Scientific Reports. 7, 9 p., 9844.

    Research output: Contribution to journalArticle

  2. Label-free optical hemogram of granulocytes enhanced by artificial neural networks

    Gupta, R., Chen, M., Malcolm, G. P. A., Hempler, N., Dholakia, K. & Powis, S. J., 13 May 2019, In : Optics Express. 27, 10, p. 13706-13720 15 p.

    Research output: Contribution to journalArticle

  3. Overcoming the speckle correlation limit to achieve a fiber wavemeter with attometer resolution

    Bruce, G. D., O'Donnell, L., Chen, M. & Dholakia, K., 15 Mar 2019, In : Optics Letters. 44, 6, p. 1367-1370

    Research output: Contribution to journalArticle

  4. Wide-field multiphoton imaging with TRAFIX

    Escobet-Montalbán, A., Wijesinghe, P., Chen, M. & Dholakia, K., 22 Feb 2019, Multiphoton Microscopy in the Biomedical Sciences XIX. Periasamy, A., So, P. T. C. & König, K. (eds.). Society of Photo-Optical Instrumentation Engineers, p. 49 9 p. 10882G. (Proceedings of SPIE; vol. 10882).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  5. Spatially offset optical coherence tomography: enhancing image contrast at depth

    Chen, M., Wijesinghe, P., Mas Soler, J., Andersen, P. E. & Dholakia, K., 20 Nov 2018, (Submitted) In : Nature Photonics.

    Research output: Contribution to journalArticle

ID: 252482462