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Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Research output: Chapter in Book/Report/Conference proceedingChapter


Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.


Original languageEnglish
Title of host publicationAntibiotic Resistance Protocols
EditorsStephen Gillespie
Place of PublicationNew York, NY
PublisherHumana Press/Springer
Number of pages10
ISBN (Electronic)9781493976386
ISBN (Print)9781493976362
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

    Research areas

  • Raman spectroscopy, Mycobacteria, Phenotypic resistance, Lipids

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