Skip to content

Research at St Andrews

Development temperature has persistent effects on muscle growth responses in gilthead sea bream

Research output: Contribution to journalArticle

Author(s)

Daniel Garcia de la Serrana Castillo, Vera Lucia Almeida Vieira-Johnston, Karl B Andree, Maria Darias, Alicia Estévez, Enric Gisbert, Ian Alistair Johnston

School/Research organisations

Abstract

Initially we characterised growth responses to altered nutritional input at the transcriptional and tissue levels in the fast skeletal muscle of juvenile gilthead sea bream. Fish reared at 21–22°C (range) were fed a commercial diet at 3% body mass d−1 (non-satiation feeding, NSF) for 4 weeks, fasted for 4d (F) and then fed to satiation (SF) for 21d. 13 out of 34 genes investigated showed consistent patterns of regulation between nutritional states. Fasting was associated with a 20-fold increase in MAFbx, and a 5-fold increase in Six1 and WASp expression, which returned to NSF levels within 16h of SF. Refeeding to satiation was associated with a rapid (<24 h) 12 to 17-fold increase in UNC45, Hsp70 and Hsp90α transcripts coding for molecular chaperones associated with unfolded protein response pathways. The growth factors FGF6 and IGF1 increased 6.0 and 4.5-fold within 16 h and 24 h of refeeding respectively. The average growth in diameter of fast muscle fibres was checked with fasting and significant fibre hypertrophy was only observed after 13d and 21d SF. To investigate developmental plasticity in growth responses we used the same experimental protocol with fish reared at either 17.5–18.5°C (range) (LT) or 21–22°C (range) (HT) to metamorphosis and then transferred to 21–22°C. There were persistent effects of development temperature on muscle growth patterns with 20% more fibres of lower average diameter in LT than HT group of similar body size. Altering the nutritional input to the muscle to stimulate growth revealed cryptic changes in the expression of UNC45 and Hsp90α with higher transcript abundance in the LT than HT groups, whereas there were no differences in the expression of MAFbx and Six1. It was concluded that myogenesis and gene expression patterns during growth are not fixed, but can be modified by temperature during the early stages of the life cycle.
Close

Details

Original languageEnglish
Article numbere51884
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - 17 Dec 2012

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Demonstration of the use of environmental DNA for the non-invasive genotyping of a bivalve mollusk, the European flat oyster (Ostrea edulis)

    Holman, L., Hollenbeck, C. M., Ashton, T. J. & Johnston, I. A., 19 Nov 2019, In : Frontiers in Genetics. 10, 7 p., 1159.

    Research output: Contribution to journalArticle

  2. Influence of feed ration size on somatic and muscle growth in landlocked dwarf and farmed Atlantic salmon Salmo salar

    Andersen, Ø., Vieira, V., Dessen, J-E. & Johnston, I. A., Apr 2019, In : Journal of Fish Biology. 94, 4, p. 614-620 7 p.

    Research output: Contribution to journalArticle

  3. Genomic tools and selective breeding in molluscs

    Hollenbeck, C. M. & Johnston, I. A., 18 Jul 2018, In : Frontiers in Genetics. 9, 15 p., 253.

    Research output: Contribution to journalReview article

  4. A collaborative European approach to accelerating translational marine science

    Brennecke, P., Ferrante, M., Johnston, I. A. & Smith, D., 5 Jul 2018, In : Journal of Marine Science and Engineering. 6, 3, 12 p., 81.

    Research output: Contribution to journalArticle

Related by journal

  1. PLoS ONE (Journal)

    Joanna Louise Kershaw (Reviewer)
    18 Mar 2019

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. PLoS ONE (Journal)

    Catharine Penelope Cross (Member of editorial board)
    1 Sep 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  3. PLoS ONE (Journal)

    Will Cresswell (Reviewer)
    23 May 2018

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  4. PLoS ONE (Journal)

    Barbara Dritschel (Member of editorial board)
    May 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  5. PLoS ONE (Journal)

    Simon Young (Reviewer)
    7 Jun 201719 Jun 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Drivers of the opioid crisis: An appraisal of financial conflicts of interest in clinical practice guideline panels at the peak of opioid prescribing

    Spithoff, S., Leece, P., Sullivan, F., Persaud, N., Belesiotis, P. & Steiner, L., 24 Jan 2020, In : PLoS ONE. 15, 1, 15 p., e0227045.

    Research output: Contribution to journalArticle

  2. Satellite derived offshore migratory movements of southern right whales (Eubalaena australis) from Australian and New Zealand wintering grounds

    Mackay, A. I., Bailleul, F., Carroll, E. L., Andrews-Goff, V., Baker, C. S., Bannister, J., Boren, L., Carlyon, K., Donnelly, D. M., Double, M., Goldsworthy, S. D., Harcourt, R., Holman, D., Lowther, A., Parra, G. J. & Childerhouse, S. J., 7 May 2020, In : PLoS ONE. 15, 5, e0231577.

    Research output: Contribution to journalArticle

  3. The shield of self-compassion: a buffer against disordered eating risk from physical appearance perfectionism

    Bergunde, L. & Dritschel, B., 13 Jan 2020, In : PLoS ONE. 15, 1, 17 p., e0227564.

    Research output: Contribution to journalArticle

  4. Under his thumb the effect of president Donald Trump’s Twitter messages on the US stock market

    Brans, H. & Scholtens, B., 11 Mar 2020, In : PLoS ONE. 15, 3, 11 p., e0229931.

    Research output: Contribution to journalArticle

ID: 43648604

Top