Skip to content

Research at St Andrews

Development temperature has persistent effects on muscle growth responses in gilthead sea bream

Research output: Contribution to journalArticle

Author(s)

Daniel Garcia de la Serrana Castillo, Vera Lucia Almeida Vieira-Johnston, Karl B Andree, Maria Darias, Alicia Estévez, Enric Gisbert, Ian Alistair Johnston

School/Research organisations

Abstract

Initially we characterised growth responses to altered nutritional input at the transcriptional and tissue levels in the fast skeletal muscle of juvenile gilthead sea bream. Fish reared at 21–22°C (range) were fed a commercial diet at 3% body mass d−1 (non-satiation feeding, NSF) for 4 weeks, fasted for 4d (F) and then fed to satiation (SF) for 21d. 13 out of 34 genes investigated showed consistent patterns of regulation between nutritional states. Fasting was associated with a 20-fold increase in MAFbx, and a 5-fold increase in Six1 and WASp expression, which returned to NSF levels within 16h of SF. Refeeding to satiation was associated with a rapid (<24 h) 12 to 17-fold increase in UNC45, Hsp70 and Hsp90α transcripts coding for molecular chaperones associated with unfolded protein response pathways. The growth factors FGF6 and IGF1 increased 6.0 and 4.5-fold within 16 h and 24 h of refeeding respectively. The average growth in diameter of fast muscle fibres was checked with fasting and significant fibre hypertrophy was only observed after 13d and 21d SF. To investigate developmental plasticity in growth responses we used the same experimental protocol with fish reared at either 17.5–18.5°C (range) (LT) or 21–22°C (range) (HT) to metamorphosis and then transferred to 21–22°C. There were persistent effects of development temperature on muscle growth patterns with 20% more fibres of lower average diameter in LT than HT group of similar body size. Altering the nutritional input to the muscle to stimulate growth revealed cryptic changes in the expression of UNC45 and Hsp90α with higher transcript abundance in the LT than HT groups, whereas there were no differences in the expression of MAFbx and Six1. It was concluded that myogenesis and gene expression patterns during growth are not fixed, but can be modified by temperature during the early stages of the life cycle.
Close

Details

Original languageEnglish
Article numbere51884
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - 17 Dec 2012

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Influence of feed ration size on somatic and muscle growth in landlocked dwarf and farmed Atlantic salmon Salmo salar

    Andersen, Ø., Vieira, V., Dessen, J-E. & Johnston, I. A., Apr 2019, In : Journal of Fish Biology. 94, 4, p. 614-620 7 p.

    Research output: Contribution to journalArticle

  2. Genomic tools and selective breeding in molluscs

    Hollenbeck, C. M. & Johnston, I. A., 18 Jul 2018, In : Frontiers in Genetics. 9, 15 p., 253.

    Research output: Contribution to journalReview article

  3. A collaborative European approach to accelerating translational marine science

    Brennecke, P., Ferrante, M., Johnston, I. A. & Smith, D., 5 Jul 2018, In : Journal of Marine Science and Engineering. 6, 3, 12 p., 81.

    Research output: Contribution to journalArticle

  4. A workflow used to design low density SNP panels for parentage assignment and traceability in aquaculture species and its validation in Atlantic salmon

    Holman, L. E., Garcia de la Serrana, D., Onoufriou, A., Hillestad, B. & Johnston, I. A., 1 Jul 2017, In : Aquaculture. 476, p. 59-64 6 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Agreement between primary care and hospital diagnosis of schizophrenia and bipolar disorder: a cross-sectional, observational study using record linkage

    O’Neill, B., Kalia, S., Aliarzadeh, B., Moineddin, R., Fung, W. L. A., Sullivan, F., Maloul, A., Bernard, S. & Greiver, M., 7 Jan 2019, In : PLoS ONE. 14, 1, 15 p., e0210214.

    Research output: Contribution to journalArticle

  2. Correlates of social role and conflict severity in wild vervet monkey agonistic screams

    Mercier, S., Déaux, E. C., van de Waal, E., Bono, A. E. J. & Zuberbuhler, K., 1 May 2019, In : PLoS One. 14, 5, 20 p., e0214640.

    Research output: Contribution to journalArticle

  3. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

    Mohamed, N., Timofeyeva, Y., Jamrozy, D., Rojas, E., Hao, L., Silmon de Monerri, N. C., Hawkins, J., Singh, G., Cai, B., Liberator, P., Sebastian, S., Donald, R. G. K., Scully, I. L., Jones, C. H., Creech, C. B., Thomsen, I., Parkhill, J., Peacock, S. J., Jansen, K. U., Holden, M. T. G. & 1 othersAnderson, A. S., 14 Jan 2019, In : PLoS ONE. 14, 1, 33 p., e0208356.

    Research output: Contribution to journalArticle

  4. Orangutans (Pongo abelii) make flexible decisions relative to reward quality and tool functionality in a multi-dimensional tool-use task

    Laumer, I. B., Auersperg, A. M. I., Bugnyar, T. & Call, J., 13 Feb 2019, In : PLoS ONE. 14, 2, 13 p.

    Research output: Contribution to journalArticle

  5. Sensitivity of multispecies maximum sustainable yields to trends in the top (marine mammals) and bottom (primary production) compartments of the southern North Sea food-web

    Stäbler, M., Kempf, A., Smout, S. & Temming, A., 28 Jan 2019, In : PLoS ONE. 14, 1, 18 p., e0210882.

    Research output: Contribution to journalArticle

Related by journal

  1. PLoS ONE (Journal)

    Catharine Penelope Cross (Member of editorial board)
    1 Sep 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  2. PLoS ONE (Journal)

    Will Cresswell (Reviewer)
    23 May 2018

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. PLoS ONE (Journal)

    Barbara Dritschel (Member of editorial board)
    May 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  4. PLoS ONE (Journal)

    Simon Young (Reviewer)
    7 Jun 201719 Jun 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  5. PLoS ONE (Journal)

    Uli Schwarz-Linek (Reviewer)
    Mar 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

ID: 43648604