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Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

Research output: Contribution to journalArticlepeer-review

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Dichomitus squalens partially tailors its molecular responses to the composition of solid wood. / Daly, Paul; López, Sara Casado; Peng, Mao; Lancefield, Christopher S; Purvine, Samuel O; Kim, Young-Mo; Zink, Erika M; Dohnalkova, Alice; Singan, Vasanth R; Lipzen, Anna; Dilworth, David; Wang, Mei; Ng, Vivian; Robinson, Errol; Orr, Galya; Baker, Scott E; Bruijnincx, Pieter C A; Hildén, Kristiina S; Grigoriev, Igor V; Mäkelä, Miia R; de Vries, Ronald P.

In: Applied and Environmental Microbiology, Vol. 20, No. 11, 11.2018, p. 4141-4156.

Research output: Contribution to journalArticlepeer-review

Harvard

Daly, P, López, SC, Peng, M, Lancefield, CS, Purvine, SO, Kim, Y-M, Zink, EM, Dohnalkova, A, Singan, VR, Lipzen, A, Dilworth, D, Wang, M, Ng, V, Robinson, E, Orr, G, Baker, SE, Bruijnincx, PCA, Hildén, KS, Grigoriev, IV, Mäkelä, MR & de Vries, RP 2018, 'Dichomitus squalens partially tailors its molecular responses to the composition of solid wood', Applied and Environmental Microbiology, vol. 20, no. 11, pp. 4141-4156. https://doi.org/10.1111/1462-2920.14416

APA

Daly, P., López, S. C., Peng, M., Lancefield, C. S., Purvine, S. O., Kim, Y-M., Zink, E. M., Dohnalkova, A., Singan, V. R., Lipzen, A., Dilworth, D., Wang, M., Ng, V., Robinson, E., Orr, G., Baker, S. E., Bruijnincx, P. C. A., Hildén, K. S., Grigoriev, I. V., ... de Vries, R. P. (2018). Dichomitus squalens partially tailors its molecular responses to the composition of solid wood. Applied and Environmental Microbiology, 20(11), 4141-4156. https://doi.org/10.1111/1462-2920.14416

Vancouver

Daly P, López SC, Peng M, Lancefield CS, Purvine SO, Kim Y-M et al. Dichomitus squalens partially tailors its molecular responses to the composition of solid wood. Applied and Environmental Microbiology. 2018 Nov;20(11):4141-4156. https://doi.org/10.1111/1462-2920.14416

Author

Daly, Paul ; López, Sara Casado ; Peng, Mao ; Lancefield, Christopher S ; Purvine, Samuel O ; Kim, Young-Mo ; Zink, Erika M ; Dohnalkova, Alice ; Singan, Vasanth R ; Lipzen, Anna ; Dilworth, David ; Wang, Mei ; Ng, Vivian ; Robinson, Errol ; Orr, Galya ; Baker, Scott E ; Bruijnincx, Pieter C A ; Hildén, Kristiina S ; Grigoriev, Igor V ; Mäkelä, Miia R ; de Vries, Ronald P. / Dichomitus squalens partially tailors its molecular responses to the composition of solid wood. In: Applied and Environmental Microbiology. 2018 ; Vol. 20, No. 11. pp. 4141-4156.

Bibtex - Download

@article{94c6338916ce4741a22b897ce7307c4f,
title = "Dichomitus squalens partially tailors its molecular responses to the composition of solid wood",
abstract = "White‐rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed‐wood forests containing both soft‐ and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi‐natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D‐NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G‐lignin rich spruce. Overall, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.",
author = "Paul Daly and L{\'o}pez, {Sara Casado} and Mao Peng and Lancefield, {Christopher S} and Purvine, {Samuel O} and Young-Mo Kim and Zink, {Erika M} and Alice Dohnalkova and Singan, {Vasanth R} and Anna Lipzen and David Dilworth and Mei Wang and Vivian Ng and Errol Robinson and Galya Orr and Baker, {Scott E} and Bruijnincx, {Pieter C A} and Hild{\'e}n, {Kristiina S} and Grigoriev, {Igor V} and M{\"a}kel{\"a}, {Miia R} and {de Vries}, {Ronald P}",
note = "PD was supported by a grant of the Netherlands Scientific Organization NWO 824.15.023 to RPdV. The Academy of Finland grant no. 308284 to MRM is acknowledged. Part of the research was performed at the Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility sponsored by the Department of Energy's Office of Biological and Environmental Research, located at the Pacific Northwest National Laboratory in Richland, WA, USA. The work conducted by the U.S. Department of Energy Joint Genome Institute (JGI), was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE‐AC02‐05CH11231. CSL was supported by the CatchBio program. ",
year = "2018",
month = nov,
doi = "10.1111/1462-2920.14416",
language = "English",
volume = "20",
pages = "4141--4156",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "11",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

AU - Daly, Paul

AU - López, Sara Casado

AU - Peng, Mao

AU - Lancefield, Christopher S

AU - Purvine, Samuel O

AU - Kim, Young-Mo

AU - Zink, Erika M

AU - Dohnalkova, Alice

AU - Singan, Vasanth R

AU - Lipzen, Anna

AU - Dilworth, David

AU - Wang, Mei

AU - Ng, Vivian

AU - Robinson, Errol

AU - Orr, Galya

AU - Baker, Scott E

AU - Bruijnincx, Pieter C A

AU - Hildén, Kristiina S

AU - Grigoriev, Igor V

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

N1 - PD was supported by a grant of the Netherlands Scientific Organization NWO 824.15.023 to RPdV. The Academy of Finland grant no. 308284 to MRM is acknowledged. Part of the research was performed at the Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility sponsored by the Department of Energy's Office of Biological and Environmental Research, located at the Pacific Northwest National Laboratory in Richland, WA, USA. The work conducted by the U.S. Department of Energy Joint Genome Institute (JGI), was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE‐AC02‐05CH11231. CSL was supported by the CatchBio program.

PY - 2018/11

Y1 - 2018/11

N2 - White‐rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed‐wood forests containing both soft‐ and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi‐natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D‐NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G‐lignin rich spruce. Overall, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.

AB - White‐rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed‐wood forests containing both soft‐ and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi‐natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D‐NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G‐lignin rich spruce. Overall, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.

U2 - 10.1111/1462-2920.14416

DO - 10.1111/1462-2920.14416

M3 - Article

C2 - 30246402

VL - 20

SP - 4141

EP - 4156

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 11

ER -

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