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Differential expression of absorptive cation-chloride-cotransporters in the intestinal and renal tissues of the European eel (Anguilla anguilla)

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Author(s)

Christopher Paul Cutler, Gordon Cramb

School/Research organisations

Abstract

Duplicate pairs of isoforms of each of the NKCC2 and the NCC absorptive cation-chloride-cotransporters have been isolated from the European eel. As with mammalian NKCC2, NKCC2! isoform mRNA expression was restricted to renal tissues, whereas NKCC2" isoform expression was present in intestine and urinary bladder. Similar to mammalian NCC, NCC! mRNA expression was also found in the kidney, whereas, expression of NCC" mRNAwas found at low levels in a number of tissues but particularly in intestine. Following 3 weeks of transfer of yellow or silver (adult life stages) eels from freshwater (FW) to seawater (SW), renal mRNA expression of NKCC2! did not change whereas NCC! expression was reduced although only significantly in silver eels. This suggests that any changes in renal sodium chloride re-absorption in SW-acclimated fish may be due to decreased NCC! cotransporter activity rather than the result of suppression of NKCC! cotransporter activity. Intestinal mRNA expression of NKCC2" generally increased following SW acclimation, although maximal increases occurred later in yellow (7 days) than silver (2 days) eels. Average levels of NKCC2" mRNA abundance in the middle intestine were 89% of those in the anterior, and this was reduced to 44% (of the level in the anterior intestine) in posterior intestine/rectum. Expression of NCC" was only found in the posterior intestine/rectum. Together these results suggest intestinal sodium chloride absorption may switch from occurring via NKCC" to NCC" as imbibed fluid travels down the intestine and the concentration of luminal potassium decreases. © 2007 Elsevier Inc. All rights reserved.
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Original languageEnglish
Pages (from-to)63-73
Number of pages11
JournalComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
Volume149
Issue number1
DOIs
Publication statusPublished - Jan 2008

    Research areas

  • cation-chloride cotransporter, ribonucleic acid, reverse transcriptase-polymerase chain reaction, NKCC (sodium potassium chloride cotransporter), NCC (sodium chloride cotransporter), mRNA expression, RACE (Rapid amplification of cDNA ends), bumetanide, thiazide, diuretics, K-CL COTRANSPORTER, ION-TRANSPORT, MOLECULAR PHYSIOLOGY, SODIUM TRANSPORTERS, FRESH-WATER, SEA-WATER, NA, FLOUNDER, ISOFORMS, TELEOST

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