Skip to content

Research at St Andrews

Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts

Research output: Chapter in Book/Report/Conference proceedingChapter


Andrew Paul Desbois, Valerie Jane Smith

School/Research organisations


Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches.


Original languageEnglish
Title of host publicationNatural Products From Marine Algae
Place of PublicationClifton New Jersey USA
Number of pages8
ISBN (Electronic)9781493926848
ISBN (Print)9781493926831
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Cellular stress responses to chronic heat shock and shell damage in temperate Mya truncata

    Sleight, V. A., Peck, L. S., Dyrynda, E. A., Smith, V. J. & Clark, M. S., 12 May 2018, In : Cell Stress and Chaperones. First Online, 15 p.

    Research output: Contribution to journalArticle

  2. Wind turbines cause chronic stress in badgers (Meles meles) in Great Britain

    Agnew, R., Smith, V. J. & Fowkes, R., Jul 2016, In : Journal of Wildlife Diseases. 52, 3, p. 459-467 9 p.

    Research output: Contribution to journalArticle

  3. Hematopoiesis and hemocytes in pancrustacean and molluscan models

    Smith, V. J., Accorsi, A. & Malagoli, D., 20 May 2016, The Evolution of the Immune System: Conservation and Diversification. Malagoli, D. (ed.). 1st ed. Academic Press/Elsevier , p. 1-28

    Research output: Chapter in Book/Report/Conference proceedingChapter

  4. Immunology of invertebrates: Cellular

    Smith, V. J., 15 Feb 2016, Encyclopedia of Life Sciences: Wiley. Wiley, 13 p. (Encyclopedia of Life Sciences).

    Research output: Chapter in Book/Report/Conference proceedingChapter

ID: 204600336