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Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts

Research output: Chapter in Book/Report/Conference proceedingChapter

Author(s)

Andrew Paul Desbois, Valerie Jane Smith

School/Research organisations

Abstract

Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches.
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Details

Original languageEnglish
Title of host publicationNatural Products From Marine Algae
Place of PublicationClifton New Jersey USA
PublisherSpringer
Pages403-410
Number of pages8
ISBN (Electronic)9781493926848
ISBN (Print)9781493926831
DOIs
StatePublished - 2015

Publication series

NameMethods in Molecular Biology
Volume1308

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