Skip to content

Research at St Andrews

Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

Li Hao, Matthew T. G. Holden, Xin Wang, Lubomira Andrew, Sabine Wellnitz, Fang Hu, Melissa Whaley, Scott Sammons, Kristen Knipe, Mike Frace, Lucy A McNamara, Paul Liberator, Annaliesa S Anderson

School/Research organisations

Abstract

Neisseria meningitidis serogroup B (MnB) was responsible for two independent meningococcal disease outbreaks at universities in the USA during 2013. The first at University A in New Jersey included nine confirmed cases reported between March 2013 and March 2014. The second outbreak occurred at University B in California, with four confirmed cases during November 2013. The public health response to these outbreaks included the approval and deployment of a serogroup B meningococcal vaccine that was not yet licensed in the USA. This study investigated the use of whole-genome sequencing(WGS) to examine the genetic profile of the disease-causing outbreak isolates at each university. Comparative WGS revealed differences in evolutionary patterns between the two disease outbreaks. The University A outbreak isolates were very closely related, with differences primarily attributed to single nucleotide polymorphisms/insertion-deletion (SNP/indel) events. In contrast, the University B outbreak isolates segregated into two phylogenetic clades, differing in large part due to recombination events covering extensive regions (>30 kb) of the genome including virulence factors. This high-resolution comparison of two meningococcal disease outbreaks further demonstrates the genetic complexity of meningococcal bacteria as related to evolution and disease virulence.

Close

Details

Original languageEnglish
Article numbere000155
Number of pages10
JournalMicrobial Genomics
Volume4
Issue number4
Early online date4 Apr 2018
DOIs
StatePublished - Apr 2018

    Research areas

  • Serogroup B Neisseria meningitidis, Disease outbreak, Multi-locus sequence typing (MLST), Whole genome sequence (WGS), Single nucleotide polymorphism (SNP), Vaccine

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Global scale dissemination of ST93: a divergent Staphylococcus aureus epidemic lineage that has recently emerged from remote Northern Australia

    van Hal, S. J., Steinig, E. J., Andersson, P., Holden, M. T. G., Harris, S. R., Nimmo, G. R., Williamson, D. A., Heffernan, H., Ritchie, S. R., Kearns, A. M., Ellington, M. J., Dickson, E., de Lencastre, H., Coombs, G. W., Bentley, S. D., Parkhill, J., Holt, D. C., Giffard, P. M. & Tong, S. Y. C. 9 Jul 2018 In : Frontiers in Microbiology. 9, 11 p., 1453

    Research output: Contribution to journalArticle

  2. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., Fodor, L., Casas Amoribieta, M., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & on behalf of the BRaDP1T consortium Jul 2018 In : Veterinary Microbiology. 220, p. 83-89 7 p.

    Research output: Contribution to journalArticle

  3. An outbreak of Streptococcus pyogenes in a mental health facility: advantage of well-timed whole-genome sequencing over emm typing

    Bergin, S. M. , Periaswamy, B. , Barkham, T. , Chua, H. C. , Mok, Y. M. , Fung, D. S. S. , Su, A. H. C. , Lee, Y. L. , Chua, M. L. I. , Ng, P. Y. , Soon, W. J. W. , Chu, C. W. , Tan, S. L. , Meehan, M. , Ang, B. S. P. , Leo, Y. S. , Holden, M. T. G. , De, P. , Hsu, L. Y. , Chen, S. L. & 2 others de Sessions, P. F. & Marimuthu, K. 9 May 2018 In : Infection Control and Hospital Epidemiology. First View, 9 p.

    Research output: Contribution to journalArticle

  4. The widespread use of topical antimicrobials enriches for resistance in Staphylococcus aureus isolated from Atopic Dermatitis patients

    Harkins, C. P., McAleer, M. A., Bennett, D., McHugh, M., Fleury, O. M., Pettigrew, K. A., Oravcová, K., Parkhill, J., Proby, C. M., Dawe, R. S., Geoghegan, J. A., Irvine, A. D. & Holden, M. T. G. 5 May 2018 (Accepted/In press) In : British Journal of Dermatology. In press

    Research output: Contribution to journalArticle

  5. Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis

    Bossé, J. T., Li, Y., Sárközi, R., Fodor, L., Lacouture, S., Gottschalk, M., Amoribieta, M. C., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & on behalf of the BRaDP1T consortium Apr 2018 In : Veterinary Microbiology. 217, p. 1-6

    Research output: Contribution to journalArticle

Related by journal

  1. Population genetic structuring of methicillin-resistant Staphylococcus aureus clone EMRSA-15 within UK reflects patient referral patterns

    Donker, T., Reuter, S., Scriberras, J., Reynolds, R., Brown, N. M., Török, M. E., James, R., Network, E. O. E. M. R., Aanensen, D. M., Bentley, S. D., Holden, M. T. G., Parkhill, J., Spratt, B. G., Peacock, S. J., Feil, E. J. & Grundmann, H. 9 Jul 2017 In : Microbial Genomics. 3, 7, 12 p., e000113

    Research output: Contribution to journalArticle

  2. Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island

    Sarovich, D. S., Chapple, S. N. J., Price, E. P., Mayo, M., Holden, M. T. G., Peacock, S. J. & Currie, B. J. 31 Aug 2017 In : Microbial Genomics. 3, 8, 7 p., e000117

    Research output: Contribution to journalArticle

  3. Microreact: visualizing and sharing data for genomic epidemiology and phylogeography

    Argimón, S., Abudahab, K., Goater, R. J. E., Fedosejev, A., Bhai, J., Glasner, C., Feil, E. J., Holden, M. T. G., Yeats, C. A., Grundmann, H., Spratt, B. G. & Aanensen, D. M. 30 Nov 2016 In : Microbial Genomics. 2, 11, 11 p.

    Research output: Contribution to journalArticle

  4. Pan-genomic perspective on the evolution of the Staphylococcus aureus USA300 epidemic

    Jamrozy, D. M., Harris, S. R., Mohamed, N., Peacock, S. J., Tan, C. Y., Parkhill, J., Anderson, A. S. & Holden, M. T. G. 31 May 2016 In : Microbial Genomics. 2, 5, p. 1-14 14 p.

    Research output: Contribution to journalArticle

ID: 252839418