Skip to content

Research at St Andrews

Efficient detection of long dsRNA in vitro and in vivo using the dsRNA binding domain from FHV B2 protein

Research output: Contribution to journalArticle


Baptiste Monsion, Marco Incarbone, Kamal Hleibieh, Vianney Poignavent, Ahmed Ghannam, Patrice Dunoyer, Laurent Daeffler, Jens Tilsner, Christophe Ritzenthaler

School/Research organisations


Double-stranded RNA (dsRNA) plays essential functions in many biological processes, including the activation of innate immune responses and RNA interference. dsRNA also represents the genetic entity of some viruses and is a hallmark of infections by positive-sense single-stranded RNA viruses. Methods for detecting dsRNA rely essentially on immunological approaches and their use is often limited to in vitro applications, although recent developments have allowed the visualization of dsRNA in vivo. Here, we report the sensitive and rapid detection of long dsRNA both in vitro and in vivo using the dsRNA binding domain of the B2 protein from Flock house virus. In vitro, we adapted the system for the detection of dsRNA either enzymatically by northwestern blotting or by direct fluorescence labeling on fixed samples. In vivo, we produced stable transgenic Nicotiana benthamiana lines allowing the visualization of dsRNA by fluorescence microscopy. Using these techniques, we were able to discriminate healthy and positive-sense single-stranded RNA virus-infected material in plants and insect cells. In N. benthamiana, our system proved to be very potent for the spatio-temporal visualization of replicative RNA intermediates of a broad range of positive-sense RNA viruses, including high- vs. low-copy number viruses.


Original languageEnglish
Article number70
Number of pages16
JournalFrontiers in Plant Science
StatePublished - 1 Feb 2018

    Research areas

  • dsRNA, Virus, Viral factories, Replication complexes, Detection, Plant, Insect, Nicotiana benthamiana

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Hitchhikers, highway tolls and roadworks: the interactions of plant viruses with the phloem

    Folimonova, S. Y. & Tilsner, J. Jun 2018 In : Current Opinion in Plant Biology. 43, p. 82-88 7 p.

    Research output: Contribution to journalReview article

  2. Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves

    Nicolas, W. J., Grison, M. S., Trépout, S., Gaston, A., Fouché, M., Cordelières, F. P., Oparka, K., Tilsner, J., Brocard, L. & Bayer, E. M. 12 Jun 2017 In : Nature Plants. 3, 17082

    Research output: Contribution to journalArticle

  3. The potential consequences for cell Signaling by a class of NOD-Like Receptor proteins (NLRs) bearing an N-terminal signal sequence

    Ryan, M. D., Roulston, C., de Felipe, P., Odon, V., Tilsner, J. & Luke, G. A. 19 May 2017 In : Journal of Cell Signaling. 2, 2, 3 p., 148

    Research output: Contribution to journalArticle

  4. Stitching organelles: organization and function of specialized membrane contact sites in plants

    Pérez-Sancho, J., Tilsner, J., Samuels, A. L., Botella, M. A., Bayer, E. M. & Rosado, A. Sep 2016 In : Trends in Cell Biology. 26, 9, p. 705-717

    Research output: Contribution to journalReview article

  5. "2A-like" signal sequences mediating translational recoding: a novel form of dual protein targeting

    Roulston, C., Luke, G. A., de Felipe, P., Ruan, L., Cope, J., Nicholson, J., Sukhodub, A., Tilsner, J. & Ryan, M. D. Aug 2016 In : Traffic. 17, 8, p. 923-939 17 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Frontiers in Plant Science (Journal)

    Tilsner, J. (Guest editor)

    Activity: Publication peer-review and editorial workEditor of research journal

Related by journal

  1. The Globodera pallida SPRYSEC effector GpSPRY-414-2 that suppresses plant defenses targets a regulatory component of the dynamic microtubule network

    Mei, Y., Wright, K. M., Haegeman, A., Bauters, L., Diaz-Granados, A., Goverse, A., Gheysen, G., Jones, J. T. & Mantelin, S. 12 Jul 2018 In : Frontiers in Plant Science. 9, 16 p., 1019

    Research output: Contribution to journalArticle

  2. Plant biodiversity drivers in Brazilian campos rupestres: insights from phylogenetic structure

    Zappi, D. C., Freire Moro, M., Meagher, T. R. & Nic Lughadha, E. 19 Dec 2017 In : Frontiers in Plant Science. 8, 15 p., 2141

    Research output: Contribution to journalArticle

  3. Viral diagnostics in plants using next generation sequencing: Computational analysis in practice

    Jones, S., Baizan-Edge, A., MacFarlane, S. & Torrance, L. 24 Oct 2017 In : Frontiers in Plant Science. 8, 10 p., 1770

    Research output: Contribution to journalReview article

  4. Specialized membrane domains of plasmodesmata, plant intercellular nanopores

    Bayer, E. M., Mongrand, S. & Tilsner, J. 30 Sep 2014 In : Frontiers in Plant Science. 5, 3 p., 507

    Research output: Contribution to journalEditorial

ID: 252168090