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Research at St Andrews

Embryonic temperature and the relative timing of muscle-specific genes during development in herring (Clupea harengus L.).

Research output: Contribution to journalArticle

Author(s)

GK Temple, NJ Cole, Ian Alistair Johnston

School/Research organisations

Abstract

Temperature influences many aspects of muscle development in herring (Clupea harengus). In Clyde herring, myofibril synthesis occurred later with respect to somite stage in embryos reared at 5 degreesC compared with 12 degreesC. The aim of the present study was to test the hypothesis that the relative timing of expression of myogenic regulatory factors (MRFs) and myosin heavy chain (MyHC) transcripts changes with developmental temperature. Reverse transcriptase/polymerase chain reaction (RT-PCR) was used to clone partial coding regions of MyoD, myogenin and MyHC from juvenile Clyde herring. Embryos were reared at 5, 8 and 12 degreesC, and the spatial and temporal expression patterns of transcripts were investigated using cRNA probes and in situ hybridisation. Antisense probes revealed a rostral-caudal progression of all three transcripts. MyoD transcription initially took place in the adaxial cells of the unsegmented, presomitic mesoderm, whereas myogenin transcription first occurred in newly formed somites. The MyHC gene transcript was not detected until approximately nine somites had formed. Since the somite stage at which the MRFs and MyHC were first expressed was independent of temperature, the hypothesis was rejected. We suggest that the effects of temperature on myofibril synthesis must occur downstream from MyHC transcription either at the level of translation or at the assembly stage.

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Details

Original languageEnglish
Pages (from-to)3629-3637
Number of pages9
JournalJournal of Experimental Biology
Volume204
Publication statusPublished - Nov 2001

    Research areas

  • herring, Clupea harengus, temperature, muscle, MyoD, myogenin, myosin heavy chain, TROUT ONCORHYNCHUS-MYKISS, SKELETAL-MUSCLE, GROWTH-CHARACTERISTICS, TRANSCRIPTION FACTORS, MYOTOMAL MUSCULATURE, ACTIN-FILAMENTS, RAINBOW-TROUT, IN-VITRO, ZEBRAFISH, MYOD

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