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Enhanced optical micromanipulation and transfection of cells using femtosecond lasers

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

DOI

Author(s)

C. T. A. Brown, X. Tsampoula, David James Stevenson, C. McDougall, F. J. Gunn-Moore, K. Dholakia

School/Research organisations

Abstract

This work investigates the use of continuous-wave and femtosecond-pulsed modes of a laser to obtain different functionalities when interacting with living cells using a single, simple, optical setup. This study demonstrates that careful control of the laser source in both spatial and/or temporal aspects of the output beam can offer major advantages in enhanced functionality and ease of use of fs-optical transfection systems. The results of this study are applicable in systems where fs-lasers are being utilized in applications that rely on nonlinear effects.
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Details

Original languageEnglish
Title of host publication2008 Digest of the IEEE/LEOS Summer Topical Meetings
PublisherIEEE
Pages75-76
Number of pages2
Volume2008
ISBN (Electronic)978-1-4244-1926-5
ISBN (Print)978-1-4244-1925-8
DOIs
Publication statusPublished - 2008
Event2008 IEEE/LEOS Summer Topical Meetings - Acapulco, Mexico
Duration: 21 Jul 2008 → 23 Jul 2008

Publication series

NameIEEE/LEOS Summer Topical Meetings
PublisherIEEE
ISSN (Print)1099-4742

Conference

Conference2008 IEEE/LEOS Summer Topical Meetings
CountryMexico
CityAcapulco
Period21/07/08 → 23/07/08

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    Tsampoula, X., Garces-Chavez, V., Comrie, M., Stevenson, D. J., Agate, B., Brown, C. T. A., Gunn-Moore, F. & Dholakia, K., 2008, SPIE. Vol. 6854. p. 8541-8541 10 p.

    Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  2. Femtosecond cellular transfection using a nondiffracting light beam

    Tsampoula, X., Garces-Chavez, V., Comrie, M., Stevenson, D. J., Agate, B., Brown, C. T. A., Gunn-Moore, F. & Dholakia, K., 30 Jul 2007, In: Applied Physics Letters. 91, 5, 3 p., 053902.

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  3. Optical transfection of mammalian cells - art. no. 61910D

    Stevenson, D. J., Agate, B., Paterson, L., Lake, T., Comrie, M., Brown, T., Riches, A., Bryant, P., Sibbett, W., Gunn-Moore, F. & Dholakia, K., 2006, Biophotonics and new therapy frontiers . Grzymala, R. & Haeberle, O. (eds.). SPIE, p. 1910-1910 8 p. (Proceeding of SPIE - The International Society for Optical Engineering; vol. 6191).

    Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  4. Femtosecond optical transfection of individual mammalian cells

    Antkowiak, M., Torres, M. L., Stevenson, D. J., Dholakia, K. & Gunn-Moore, F. J., Jun 2013, In: Nature Protocols. 8, 6, p. 1216-1233

    Research output: Contribution to journal › Article › peer-review

  5. Enhancement and optimization of plasmid expression in femtosecond optical transfection

    Praveen, B. B., Stevenson, D. J., Antkowiak, M., Dholakia, K. & Gunn-Moore, F. J., Apr 2011, In: Journal of Biophotonics. 4, 4, p. 229-235 7 p.

    Research output: Contribution to journal › Article › peer-review

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