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Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA)

Research output: Contribution to journalArticlepeer-review

DOI

Standard

Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA). / Lowe, Phillip T.; Dall'Angelo, Sergio; Fleming, Ian N.; Piras, Monica; Zanda, Matteo; O'Hagan, David.

In: Organic & Biomolecular Chemistry, Vol. 17, No. 6, 14.02.2019, p. 1480-1486.

Research output: Contribution to journalArticlepeer-review

Harvard

Lowe, PT, Dall'Angelo, S, Fleming, IN, Piras, M, Zanda, M & O'Hagan, D 2019, 'Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA)', Organic & Biomolecular Chemistry, vol. 17, no. 6, pp. 1480-1486. https://doi.org/10.1039/C8OB03150A

APA

Lowe, P. T., Dall'Angelo, S., Fleming, I. N., Piras, M., Zanda, M., & O'Hagan, D. (2019). Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA). Organic & Biomolecular Chemistry, 17(6), 1480-1486. https://doi.org/10.1039/C8OB03150A

Vancouver

Lowe PT, Dall'Angelo S, Fleming IN, Piras M, Zanda M, O'Hagan D. Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA). Organic & Biomolecular Chemistry. 2019 Feb 14;17(6):1480-1486. https://doi.org/10.1039/C8OB03150A

Author

Lowe, Phillip T. ; Dall'Angelo, Sergio ; Fleming, Ian N. ; Piras, Monica ; Zanda, Matteo ; O'Hagan, David. / Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA). In: Organic & Biomolecular Chemistry. 2019 ; Vol. 17, No. 6. pp. 1480-1486.

Bibtex - Download

@article{59f9293180214a798a6202acd82ba578,
title = "Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA)",
abstract = "Prostate cancer represents a major public health threat as it is one of the most common male cancers worldwide. The prostate-specific membrane antigen (PSMA) is highly over-expressed in prostatic cancer cells in a manner that correlates with both tumour stage and clinical outcome. As such, PSMA has been identified as an attractive target for both imaging and treatment of prostate cancer. In recent years the focus on urea-based peptidomimetic inhibitors of the PSMA (representing low molecular weight/high affinity binders) has intensified as they have found use in the clinical imaging of prostate tumours. Reported herein are the design, synthesis and evaluation of a new fluorinated PSMA targeting small-molecule, FDA-PEG-GUL, which possesses the Glu-NH-CO-NH-Lys pharmacophore conjugated to a 5′-fluorodeoxy-adenosine unit. Inhibition assays were performed with FDA-PEG-GUL which revealed that it inhibits the PSMA in the nanomolar range. Additionally, it has been purposely designed so that it can be produced using the fluorinase enzyme from its chlorinated precursor, allowing for the enzymatic synthesis of radiolabelled [18F]FDA-PEG-GUL via a nucleophilic reaction that takes place in experimentally advantageous conditions (in water at neutral pH and at ambient temperature). Specific binding of [18F]FDA-PEG-GUL to PSMA expressing cancer cells was demonstrated, validating it as a promising PSMA diagnostic tool. This work establishes a successful substrate scope expansion for the fluorinase and demonstrates its first application towards targeting the PSMA.",
author = "Lowe, {Phillip T.} and Sergio Dall'Angelo and Fleming, {Ian N.} and Monica Piras and Matteo Zanda and David O'Hagan",
note = "We thank the Engineering and Physical Sciences Research Council, UK, for a research grant (EP/M01262X/1).",
year = "2019",
month = feb,
day = "14",
doi = "10.1039/C8OB03150A",
language = "English",
volume = "17",
pages = "1480--1486",
journal = "Organic & Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Enzymatic radiosynthesis of a 18F-Glu-Ureido-Lys ligand for the prostate-specific membrane antigen (PSMA)

AU - Lowe, Phillip T.

AU - Dall'Angelo, Sergio

AU - Fleming, Ian N.

AU - Piras, Monica

AU - Zanda, Matteo

AU - O'Hagan, David

N1 - We thank the Engineering and Physical Sciences Research Council, UK, for a research grant (EP/M01262X/1).

PY - 2019/2/14

Y1 - 2019/2/14

N2 - Prostate cancer represents a major public health threat as it is one of the most common male cancers worldwide. The prostate-specific membrane antigen (PSMA) is highly over-expressed in prostatic cancer cells in a manner that correlates with both tumour stage and clinical outcome. As such, PSMA has been identified as an attractive target for both imaging and treatment of prostate cancer. In recent years the focus on urea-based peptidomimetic inhibitors of the PSMA (representing low molecular weight/high affinity binders) has intensified as they have found use in the clinical imaging of prostate tumours. Reported herein are the design, synthesis and evaluation of a new fluorinated PSMA targeting small-molecule, FDA-PEG-GUL, which possesses the Glu-NH-CO-NH-Lys pharmacophore conjugated to a 5′-fluorodeoxy-adenosine unit. Inhibition assays were performed with FDA-PEG-GUL which revealed that it inhibits the PSMA in the nanomolar range. Additionally, it has been purposely designed so that it can be produced using the fluorinase enzyme from its chlorinated precursor, allowing for the enzymatic synthesis of radiolabelled [18F]FDA-PEG-GUL via a nucleophilic reaction that takes place in experimentally advantageous conditions (in water at neutral pH and at ambient temperature). Specific binding of [18F]FDA-PEG-GUL to PSMA expressing cancer cells was demonstrated, validating it as a promising PSMA diagnostic tool. This work establishes a successful substrate scope expansion for the fluorinase and demonstrates its first application towards targeting the PSMA.

AB - Prostate cancer represents a major public health threat as it is one of the most common male cancers worldwide. The prostate-specific membrane antigen (PSMA) is highly over-expressed in prostatic cancer cells in a manner that correlates with both tumour stage and clinical outcome. As such, PSMA has been identified as an attractive target for both imaging and treatment of prostate cancer. In recent years the focus on urea-based peptidomimetic inhibitors of the PSMA (representing low molecular weight/high affinity binders) has intensified as they have found use in the clinical imaging of prostate tumours. Reported herein are the design, synthesis and evaluation of a new fluorinated PSMA targeting small-molecule, FDA-PEG-GUL, which possesses the Glu-NH-CO-NH-Lys pharmacophore conjugated to a 5′-fluorodeoxy-adenosine unit. Inhibition assays were performed with FDA-PEG-GUL which revealed that it inhibits the PSMA in the nanomolar range. Additionally, it has been purposely designed so that it can be produced using the fluorinase enzyme from its chlorinated precursor, allowing for the enzymatic synthesis of radiolabelled [18F]FDA-PEG-GUL via a nucleophilic reaction that takes place in experimentally advantageous conditions (in water at neutral pH and at ambient temperature). Specific binding of [18F]FDA-PEG-GUL to PSMA expressing cancer cells was demonstrated, validating it as a promising PSMA diagnostic tool. This work establishes a successful substrate scope expansion for the fluorinase and demonstrates its first application towards targeting the PSMA.

U2 - 10.1039/C8OB03150A

DO - 10.1039/C8OB03150A

M3 - Article

VL - 17

SP - 1480

EP - 1486

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

SN - 1477-0520

IS - 6

ER -

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