Skip to content

Research at St Andrews

ERp29, a general endoplasmic reticulum marker, is highly expressed throughout the brain

Research output: Contribution to journalArticle

DOI

Author(s)

Jennifer C MacLeod, Rod J Sayer, John M Lucocq, Michael J Hubbard

School/Research organisations

Abstract

ERp29 is a recently discovered resident of the endoplasmic reticulum (ER) that is abundant in brain and most other mammalian tissues. Investigations of nonneural secretory tissues have implicated ERp29 in a major role producing export proteins, but a molecular activity remains wanting for this functional orphan. Intriguingly, ERp29 appears to be heavily utilized in the cerebellum, a brain region not conventionally regarded as neurosecretory. To elucidate this functional quandary, we used immunochemical approaches to characterize the regional, cellular, and subcellular distributions of ERp29 in rat brain. Immunohistochemistry revealed ubiquitous expression in neuronal and nonneuronal cells, with a distinctive variation in somatic ERp29 levels. Highly expressing cells were found in diverse locations, implying that ERp29 is not biased towards the cerebellum functionally. Using immunolocalization data mined from the literature, a proteomic profile was developed to assess the functional significance of ERp29's characteristic expression pattern. Surprisingly, ERp29 correlated poorly with classical markers of neurosecretion, but strongly with a variety of major membrane proteins. Together with immunogold localization of ERp29 to somatic ER, these observations led to a novel hypothesis that ERp29 is involved primarily in production of endomembrane proteins rather than proteins destined for export. This study establishes ERp29 as a general ER marker for brain cells and provides a stimulating clue about ERp29's enigmatic function. ERp29 appears to have broad significance for neural pathophysiology, given its ubiquitous distribution and prominence in brain over classical ER residents like BiP and protein disulfide isomerase.
Close

Details

Original languageEnglish
Pages (from-to)29-42
Number of pages14
JournalThe Journal of Comparative Neurology
Volume477
Issue number1
DOIs
Publication statusPublished - 6 Sep 2004

    Research areas

  • Animals, Biological Markers, Brain, Endoplasmic Reticulum, Heat-Shock Proteins, Immunoblotting, Immunohistochemistry, Male, Microscopy, Immunoelectron, Neurons, Protein Array Analysis, Rats, Rats, Sprague-Dawley, Subcellular Fractions

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The invasive cell coat at the microsporidian Trachipleistophora hominis-host cell interface contains secreted hexokinases

    Ferguson, S. & Lucocq, J., Apr 2019, In : Microbiology Open. 8, 4, 19 p., e00696.

    Research output: Contribution to journalArticle

  2. Enhanced imaging of lipid rich nanoparticles embedded in methylcellulose films for transmission electron microscopy using mixtures of heavy metals

    Asadi, J., Ferguson, S., Raja, H., Hacker, C., Marius, P., Ward, R., Pliotas, C., Naismith, J. H. & Lucocq, J., Aug 2017, In : Micron. 99, p. 40-48 9 p.

    Research output: Contribution to journalArticle

  3. Quantifying Golgi structure using EM: combining volume-SEM and stereology for higher throughput

    Ferguson, S., Steyer, A. M., Mayhew, T. M., Schwab, Y. & Lucocq, J. M., Jun 2017, In : Histochemistry and Cell Biology. 147, 6, p. 653-669 17 p.

    Research output: Contribution to journalReview article

  4. Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis

    Freibert, S-A., Goldberg, A. V., Hacker, C., Molik, S., Dean, P., Williams, T. A., Nakjang, S., Long, S., Sendra, K., Bill, E., Heinz, E., Hirt, R. P., Lucocq, J. M., Embley, T. M. & Lill, R., 4 Jan 2017, In : Nature Communications. 8, 12 p., 13932.

    Research output: Contribution to journalArticle

  5. Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6

    Elfick, A., Rischitor, G., Mouras, R., Azfer, A., Lungaro, L., Uhlarz, M., Herrmannsdörfer, T., Lucocq, J. M., Gamal, W., Bagnaninchi, P., Semple, S. & Salter, D. M., 4 Jan 2017, In : Scientific Reports. 7, 8 p., 39755.

    Research output: Contribution to journalArticle

Related by journal

  1. Corticosterone and Dehydroepiandrosterone Have Opposing Effects on Adult Neuroplasticity in the Avian Song Control System

    Newman, A. E. M., MacDougall-Shackleton, S. A., An, Y-S., Kriengwatana, B. & Soma, K. K., 15 Sep 2010, In : The Journal of Comparative Neurology. 518, 18, p. 3662-3678 17 p.

    Research output: Contribution to journalArticle

  2. Dopamine and histamine in the developing stomatogastric system of the lobster Homarus americanus

    Pulver, S. R., Thirumalai, V., Richards, K. S. & Marder, E., 4 Aug 2003, In : The Journal of Comparative Neurology. 462, 4, p. 400-14 15 p.

    Research output: Contribution to journalArticle

  3. Neuromodulatory complement of the pericardial organs in the embryonic lobster, Homarus americanus

    Pulver, S. R. & Marder, E., 9 Sep 2002, In : The Journal of Comparative Neurology. 451, 1, p. 79-90 12 p.

    Research output: Contribution to journalArticle

  4. Orcokinin peptides in developing and adult crustacean stomatogastric nervous systems and pericardial organs

    Li, L., Pulver, S. R., Kelley, W. P., Thirumalai, V., Sweedler, J. V. & Marder, E., 12 Mar 2002, In : The Journal of Comparative Neurology. 444, 3, p. 227-44 18 p.

    Research output: Contribution to journalArticle

ID: 158202991

Top